vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ16.167vavilov-637Research ArticleСелекция растений на иммунитет и продуктивностьPlant breeding for immunity and performanceСоздание исходного материала яровой мягкой пшеницы для селекции на устойчивость к стеблевой ржавчине (Puccinia graminis Pers. f. sp. tritici), в том числе и к расе Ug99, в РоссииThe development of initial material of spring common wheat for breeding for resistance to stem rust (Puccinia graminis Pers. f. sp. tritici), uncluding race Ug99, in RussiaЛапочкинаИ. Ф.LapochkinaI. F.

Московская область

Moscow Oblast

inna-lapochkina@yandex.ruБарановаО. А.BaranovaO. A.

Санкт-Петербург, Пушкин

Saint- Petersburg, Pushkin

ШаманинВ. П.ShamaninV. P.

Омск

Omsk

ВолковаГ. В.VolkovaG. V.

Краснодар

Krasnodar

ГайнуллинН. Р.GainullinN. R.

Московская область

Moscow Oblast

АнисимоваА. В.AnisimovaA. V.

Санкт-Петербург, Пушкин

ГалингерД. Н.GalingerD. N.

Московская область

Moscow Oblast

ЛазареваЕ. Н.LazarevaE. N.

Московская область

Moscow Oblast

ГладковаЕ. В.GladkovaE. V.

Краснодар

Krasnodar

ВагановаО. Ф.VaganovaO. F.

Краснодар

Krasnodar

Московский научно-исследовательский институт сельского хозяйства «Немчиновка»РоссияMoscow Agricultural Research Institute «Nemchinovka»Russian FederationФедеральное государственное бюджетное научное учреждение «Всероссийский институт защиты растений»РоссияAll-Russian Institute of Plant ProtectionRussian FederationФедеральное государственное бюджетное образовательное учреждение высшего образования «Омский государственный аграрный университет им. П.А. Столыпина»РоссияOmsk State Agrarian UniversityRussian FederationФедеральное государственное бюджетное научное учреждение «Всероссийский научно-исследовательский институт биологической защиты растений»РоссияAll-Russian Research Institute of Plant Biological ProtectionRussian Federation201613082016203320328Copyright © Лапочкина И.Ф., Баранова О.А., Шаманин В.П., Волкова Г.В., Гайнуллин Н.Р., Анисимова А.В., Галингер Д.Н., Лазарева Е.Н., Гладкова Е.В., Ваганова О.Ф., 20162016Лапочкина И.Ф., Баранова О.А., Шаманин В.П., Волкова Г.В., Гайнуллин Н.Р., Анисимова А.В., Галингер Д.Н., Лазарева Е.Н., Гладкова Е.В., Ваганова О.Ф.Lapochkina I.F., Baranova O.A., Shamanin V.P., Volkova G.V., Gainullin N.R., Anisimova A.V., Galinger D.N., Lazareva E.N., Gladkova E.V., Vaganova O.F.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/637

Доноры устойчивости мягкой пшеницы к расе Ug99 стебле- вой ржавчины (селекционные линии GТ 96/90, 113/00i-4 и 119/4-06rw) вовлечены в скрещивания между собой с использованием методов ступенчатых скрещиваний и бек-кроссирования. При создании гибридов с яровым образом жизни для беккроссирования использовали донор устойчивости 113/00i-4 (Sr2, Sr36, Sr39, Sr40, Sr44, Sr47) и образец 145/05i, устойчивый к бурой ржавчине, но восприимчивый к Ug99 в условиях Центрального региона России. Из полученных гибридных семей F4–F5 и беккроссного потомства BC1F3- BC2F2- BC3F2 с использованием молекулярных маркеров было выделено 137 растений с гомозиготным состоянием аллелей 2–5 генов устойчивости к стеблевой ржавчине. Потомство этих индивидуальных растений в Северо-Кавказском и Западно-Сибирском регионах России оценено по устойчивости к природным популяциям стеблевой и бурой ржавчины, а в Центральном регионе – к мучнистой росе. Устойчивые к этим заболеваниям линии оценены по развитию других хозяйственно ценных признаков: высоте растения, числу дней до колошения, продуктивности колоса, массе 1000 зерен, содержанию белка и клейковины в зерне. Отобрана 71 линия яровой пшеницы с групповой устойчивостью к двум–трем болезням и лучшим развитием хозяйственно ценных признаков для Центрального региона и 20 линий для Западной Сибири с целью дальнейшего испытания конкурентоспособности с другими лучшими сортами и линиями в селекционных питомниках. В результате работы создан оригинальный исходный материал яровой пшеницы с несколькими генами устойчивости к расе Ug99 стеблевой ржавчины.

Donors of resistance of common wheat to stem rust race Ug99 (breeding lines GT 96/90, 113/00i-4 and 119/4-06rw) are involved in cross breeding with the use of the step crossing and backcrossing method. While developing hybrids with the spring mode of life, donor of resistance 113/00i-4 (Sr2, Sr36, Sr39, Sr40, Sr44, Sr47) and accession 145/05i, which is resistant to leaf rust under the conditions of the RF Central Region but susceptible to Ug99, were used for backcrossing, 137 individual plants with the homozygotic state of alleles of 2–5 genes of resistance to stem rust were selected from the obtained hybrid families F4–F5 and backcross progeny BC1F3-BC2F2-BC3F2 by means of molecular markers. The progeny of these individual plants was tested in the North Caucasian and West Siberian regions of the RF for natural populations of stem and leaf rust and for powder mildew in the Central Region. The lines resistant to these diseases were estimated as to the other economically valuable features: plant height, number of days before heading, ear productivity, weight of 1,000 grains, protein and gluten content in grain, 71 spring wheat line with multiple resistance to two or three diseases and the development of best agronomic characters were selected for the Central region and 20 lines were selected for West Siberia for further testing of their competitiveness with the best varieties and lines in the breeding nurseries. As a result of the work, original source material was developed with several spring wheat resistance genes to Ug99.

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The authors declare that there are no conflicts of interest present.