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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vavilov</journal-id><journal-title-group><journal-title xml:lang="ru">Вавиловский журнал генетики и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Vavilov Journal of Genetics and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2500-3259</issn><publisher><publisher-name>Institute of Cytology and Genetics of Siberian Branch of the RAS</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18699/VJ16.106</article-id><article-id custom-type="elpub" pub-id-type="custom">vavilov-644</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Генетика и цитогенетика растений. ОРИГИНАЛЬНОЕ ИССЛЕДОВАНИЕ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>Plant genetics and cytogenetics. ORIGINAL ARTICLE</subject></subj-group></article-categories><title-group><article-title>Генетическое разнообразие канареечника (Phalaris arundinaceae L.), выявленное с помощью изоферментных маркеров</article-title><trans-title-group xml:lang="en"><trans-title>The genetic diversity of reed canarygrass (Phalaris arundinaceae L.) assessed by isozyme markers</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Юдина</surname><given-names>Р. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Yudina</surname><given-names>R. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новосибирск</p></bio><bio xml:lang="en"><p>Novosibirsk</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Хлесткина</surname><given-names>Е. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Khlestkina</surname><given-names>E. K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новосибирск</p></bio><bio xml:lang="en"><p>Novosibirsk</p></bio><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Федеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук»<country>Россия</country></aff><aff xml:lang="en">Institute of Cytology and Genetics SB RAS<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Федеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук»;&#13;
Федеральное государственное автономное образовательное учреждение высшего образования «Новосибирский национальный исследовательский государственный университет»<country>Россия</country></aff><aff xml:lang="en">Institute of Cytology and Genetics SB RAS;&#13;
Novosibirsk State University<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2016</year></pub-date><pub-date pub-type="epub"><day>14</day><month>08</month><year>2016</year></pub-date><volume>20</volume><issue>3</issue><fpage>364</fpage><lpage>369</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Юдина Р.С., Хлесткина Е.К., 2016</copyright-statement><copyright-year>2016</copyright-year><copyright-holder xml:lang="ru">Юдина Р.С., Хлесткина Е.К.</copyright-holder><copyright-holder xml:lang="en">Yudina R.S., Khlestkina E.K.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vavilov.elpub.ru/jour/article/view/644">https://vavilov.elpub.ru/jour/article/view/644</self-uri><abstract><p>Канареечник тростниковидный (Phalaris arundinaceae L.) – многолетний корневищный дикорастущий злак. Эта ценная кормовая и декоративная культура, широко распространен­ ная по всем континентам (кроме Антарктиды), рассматрива­ ется в последнее время во многих европейских странах еще и как перспективный источник биотоплива. Основными достоинствами канареечника являются высокая продуктив­ ность биомассы, экологическая стабильность и устойчивость к абиотическому стрессу, высокая семенная продуктивность. По сравнению с большинством других дикорастущих расте­ ний тростниковидный канареечник изучен слабо. В настоя­ щей работе с помощью изоферментных маркеров изоцитрат-дегидрогеназы (ИДГ), глутаматдегидрогеназы (ГДГ), малатде-гидрогеназы (НАД-МДГ), малик-энзима (МЭ) и шикиматде­ гидрогеназы (ШДГ) изучена коллекция канареечника тростниковидного, представленная 42 популяциями луговых биоценозов нескольких регионов России и ряда других стран. Данные ферменты тростниковидного канареечника впервые описаны в настоящем исследовании с генетической точки зрения. Установлено, что ИДГ и МЭ кодируются каждый одним локусом (Idh и Me соответственно), ШДГ и ГДГ имеют дигенный контроль (локусы Skdh1 и Skdh2, Gdh1 и Gdh2 соответственно). МДГ соответствуют 3 локуса (Mdh1, Mdh2 и Mdh3). Число аллелей на локус варьировало от 1 до 3. Высокая активность в различных органах и тканях, а также кодоминантный тип наследования делают изоферменты удобными маркерами в эколого- и популяционно-генетических исследованиях, особенно у видов растений с неизученным геномом, к кото­ рым относится и канареечник тростниковидный. В статье обсуждаются результаты кластерного анализа, выполненного на основе данных исследования изоферментов в коллекции канареечника. Кластерный анализ выявил 22 различные группы. Сделан вывод о том, что степень генетического сходства образцов из изученной коллекции не связана с географическим происхождением материала.</p></abstract><trans-abstract xml:lang="en"><p>The reed canarygrass (Phalaris arundinacea L.) is a wild-growing rhizomatous perennial cereal plant. This is a valuable forage and decorative crop, widely spread over all the continents except for Antarctic. So far, the reed canarygrass has become rather demanded in many European countries as a source of bioenergy. Among the major advantages of the reed canarygrass are high biomass yield, ecological stability, tolerance, and high seed production. Similar to most of wild-growing plants, the reed canarygrass is poorly studied. In the current study, the genetic diversity of a reed canarygrass collection (42 populations collected in meadow biocenoses of several regions in Russia and some other countries) was investigated using isozyme markers IDH (isocitrate dehydrogenase), GDH (glutamate dehydrogenase), MDH (malate dehydrogenase), ME (malic enzyme), and SKDH (shikimate dehydrogenase). Genetic control of these enzymes was determined in reed canarygrass for the first time. IDH and ME are controlled each by one locus (Idh and Me, respectively), SKDH and GDH have digenic control (loci Skdh1 and -2; Gdh1 and -2, respectively), MDH is controlled by 3 loci (Mdh1, -2 and -3). A number of alleles per locus varied from 1 to 3. High activities in different organs and tissues, as well as codominant inheritance make isozymes convenient genetic markers in various studies into ecological and population genetics, especially in plant species, like reed canarygrass, with unsequenced genome. Cluster analysis based on isozyme data distinguished 22 diverse groups. The degree of genetic similarity was not related with geographical origin of the material.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>канареечник тростниковидный (Phalaris arundinaceae L.)</kwd><kwd>источник биотоплива</kwd><kwd>генетическое разнообразие</kwd><kwd>изоферментные маркеры</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Phalaris</kwd><kwd>canarygrass</kwd><kwd>bioenergy source</kwd><kwd>genetic diversity</kwd><kwd>genetic markers</kwd></kwd-group><funding-group xml:lang="en"><funding-statement>Mrs O.V. Zaharova, State Budget Programme</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Adams W.T., Joly R.J. Genetics of allozyme variants in loblolly pine. J. Heredity. 1980a;71:33-40.</mixed-citation><mixed-citation xml:lang="en">Adams W.T., Joly R.J. 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