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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vavilov</journal-id><journal-title-group><journal-title xml:lang="ru">Вавиловский журнал генетики и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Vavilov Journal of Genetics and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2500-3259</issn><publisher><publisher-name>Institute of Cytology and Genetics of Siberian Branch of the RAS</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18699/VJ16.183</article-id><article-id custom-type="elpub" pub-id-type="custom">vavilov-808</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ГЕНОМИКА И ТРАНСКРИПТОМИКА</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>PLANT GENETICS AND BREEDING</subject></subj-group></article-categories><title-group><article-title>Результаты использования клеточных технологий в создании новых сортов ячменя, устойчивых к токсичности алюминия и засухе</article-title><trans-title-group xml:lang="en"><trans-title>Results of using cell technologies for creation of new barley varieties resistant against aluminum toxicity and drought</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Шуплецова</surname><given-names>О. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Shupletsova</surname><given-names>O. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Киров, Россия</p></bio><bio xml:lang="en"><p>Kirov, Russia</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Щенникова</surname><given-names>И. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Shchennikova</surname><given-names>I. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Киров, Россия</p></bio><bio xml:lang="en"><p>Kirov, Russia</p></bio><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Федеральное государственное бюджетное научное учреждение «Зональный научно-исследовательский институт сельского хозяйства Северо-Востока им. Н.В. Рудницкого»&#13;
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Федеральное государственное бюджетное образовательное учреждение высшего образования «Вятский государственный университет»<country>Россия</country></aff><aff xml:lang="en">North-East Agricultural Research Institute&#13;
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Vyatka State University<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Федеральное государственное бюджетное научное учреждение «Зональный научно-исследовательский институт сельского хозяйства Северо-Востока им. Н.В. Рудницкого»<country>Россия</country></aff><aff xml:lang="en">North-East Agricultural Research Institute<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2016</year></pub-date><pub-date pub-type="epub"><day>30</day><month>12</month><year>2016</year></pub-date><volume>20</volume><issue>5</issue><fpage>623</fpage><lpage>628</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Шуплецова О.Н., Щенникова И.Н., 2016</copyright-statement><copyright-year>2016</copyright-year><copyright-holder xml:lang="ru">Шуплецова О.Н., Щенникова И.Н.</copyright-holder><copyright-holder xml:lang="en">Shupletsova O.N., Shchennikova I.N.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vavilov.elpub.ru/jour/article/view/808">https://vavilov.elpub.ru/jour/article/view/808</self-uri><abstract><p>Представлены результаты изучения хозяйственно ценных свойств регенерантных линий ячменя, полученных методом клеточной селекции на селективных средах in vitro с ионами алюминия, водорода и полиэтиленгликолем. Созданы генотипы, превышающие исходный и стандартный сорта: по уровню индекса длины корней (регенеранты 0,9–1,2 %, стандарт 0,8 %) и засухоустойчивости (регенеранты 17,8–45,2 %, стандарт 8,5 %) – в лабораторных исследованиях, по продуктивным показателям – в вегетационном испытании (повышение всхожести на 12 %, продуктивной кустистости – на 21 %, озерненности колоса – в 2,3 раза, массы зерна с растения – в 1,5 раза). Показатель массы зерна с одного растения коррелировал с уровнем подщелачивания в зоне ризосферы исследуемых растений в стрессовых условиях вегетационного опыта (r = 0,908). В результате иммунологической оценки на инфекционном фоне выявлены регенерантные линии, характеризующиеся низкой степенью поражения фитопатогенными грибами. Преимущество по урожайности генотипов регенерантного происхождения перед исходными сортами и стандартами особенно проявилось в 2010 г. в провокационных условиях кислых почв и недостатка влаги, когда в полевых испытаниях регенерантные генотипы стабильно обеспечивали на 10,0–43,2 % более высокую урожайность, чем стандартный сорт. Доля регенерантных линий на этапе конкурсного сортоиспытания возросла с 8,3 (2006 г.) до 32,4 % (2014 г.). Регенеранты, обладающие высокой комбинационной способностью, используются в качестве родительских форм при скрещивании. Получены новые сорта ячменя Форвард и Бионик на основе регенерантных линий 917-01 и 496-07. В условиях эдафического стресса (рН 3,8–4,5; Аl3+ 0,5–9,6 мг/100 г почвы) урожайность сортов достигала: Форвард – 5,5 т/га, Бионик – 6,6 т/ га, превышение над стандартом составляло 113–128 %.</p></abstract><trans-abstract xml:lang="en"><p>Results of a study of economically valuable parameters of barley regenerant lines obtained by cell selection on selective media with aluminum, hydrogen ions and polyethylene glycol are presented. Genotypes superior to the initial variety and standard variety have been identified under laboratory conditions: root length index (regenerants: 0.9–1.2 %; standard: 0.8 %) and drought resistance (regenerants: 17.8–45.2 %; standard: 8.5 %); productive parameters under growth chamber conditions (12 % increase in germination ability, 21 % increase in productive plant stand, 2.3- fold increase in grain number per ear, 1.5-fold increase in weight of grains per plant). The parameter ”weight of grains per plant” correlates with the level of alkalization of the rhizosphere zone of the investigated plants under stressful conditions in growth chamber experiments (r = 0.908). Regenerant pants are typically much less affected by phytopathogenic fungi. The genotypes screened in selective systems in vitro had advantages over the initial varieties and standard varieties in productivity, especially under provocative conditions of acid soils and moisture deficit in 2010. As a result of field tests, the genotypes of regenerant origin were identified that had consistently shown a 10.0–43.2 % higher productivity than the standard variety over several years. The proportion of varieties regenerated at the stage of competitive variety trials increased from 8.3 % (2006) to 32.4 % (2014). The varieties regenerated with high combining ability are used as the parental forms in crosses. New barley varieties Forward and Bionik have been developed on the basis of regenerant lines 917-01 and 496-07. Under edaphic stress (pH 3.8–4.5; Аl3+ 0.5–9.6 mg/100 g soil), Forward has productivity up to 5.5 t/ha and Bionik, 6.6 t/ha; which is 113–128 % higher than the standard.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>ячмень</kwd><kwd>алюминий</kwd><kwd>засуха</kwd><kwd>клеточная селекция</kwd><kwd>регенерант</kwd><kwd>оценка</kwd><kwd>алюмоустойчивость</kwd><kwd>засухоустойчивость</kwd><kwd>урожайность</kwd><kwd>сорт.</kwd></kwd-group><kwd-group xml:lang="en"><kwd>barley</kwd><kwd>aluminum</kwd><kwd>drought</kwd><kwd>cell selection</kwd><kwd>regenerant</kwd><kwd>estimation</kwd><kwd>aluminum resistance</kwd><kwd>drought resistance</kwd><kwd>productivity</kwd><kwd>variety</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Butenko R.G. 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