vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ16.185vavilov-810Research ArticleГенетические ресурсы растенийPlant genetic resourcesМетаболомный подход к сравнительному анализу диких и культурных видов овса (Avena L.)The metabolomic approach to the comparative analysis of wild and cultivated species of oats (Avena L.)ЛоскутовИ. Г.LoskutovI. G.

Санкт-Петербург, Россия

St.-Petersburg, Russia

i.loskutov@vir.nw.ruШеленгаТ. В.ShelengaT. V.

Санкт-Петербург, Россия

St.-Petersburg, Russia

КонаревА. В.KonarevA. V.

Санкт-Петербург, Россия

St.-Petersburg, Russia

ШавардаА. Л.ShavardaA. I.

Санкт-Петербург, Россия

St.-Petersburg, Russia

БлиноваЕ. В.BlinovaE. V.

Санкт-Петербург, Россия

St.-Petersburg, Russia

ДзюбенкоН. И.DzubenkoN. I.

Санкт-Петербург, Россия

St.-Petersburg, Russia

Федеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова» Федеральное государственное бюджетное образовательное учреждение высшего образования «Санкт-Петербургский государственный университет»РоссияThe Federal Research Center All-Russian N.I. Vavilov Institute of Plant Genetic Resources (VIR) St.-Petersburg State UniversityRussian FederationФедеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова»РоссияThe Federal Research Center All-Russian N.I. Vavilov Institute of Plant Genetic Resources (VIR)Russian FederationФедеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова» Федеральное государственное бюджетное образовательное учреждение высшего образования «Санкт-Петербургский государственный университет» Федеральное государственное бюджетное учреждение науки «Ботанический институт им. В.Л. Комарова Российской академии наук», Санкт-Петербург, РоссияРоссияThe Federal Research Center All-Russian N.I. Vavilov Institute of Plant Genetic Resources (VIR) St.-Petersburg State University V.L. Komarov Botanical Institute of the Russian Academy of SciencesRussian Federation201630122016205636642Copyright © Лоскутов И.Г., Шеленга Т.В., Конарев А.В., Шаварда А.Л., Блинова Е.В., Дзюбенко Н.И., 20162016Лоскутов И.Г., Шеленга Т.В., Конарев А.В., Шаварда А.Л., Блинова Е.В., Дзюбенко Н.И.Loskutov I.G., Shelenga T.V., Konarev A.V., Shavarda A.I., Blinova E.V., Dzubenko N.I.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/810

Исследовались метаболомные спектры зерновок диких и культивируемых форм (сортов) овса (Avena L.). Материалом для изучения служили образцы семян из коллекции ВИР. Метаболомные исследования (анализ метаболома) проводили методом газожидкостной хроматографии с масс-спектрометрией (ГЖХ-МС) на хроматографе Agilent 6850 (CША). Анализировали состав и содержание органических и жирных кислот, аминокислот, многоатомных спиртов и сахаров. Установлено, что у сортов по сравнению с дикими видами сузился (в ряде случаев существенно) диапазон колебания значений содержания изученных групп соединений. Наряду с резким увеличением содержания олеиновой кислоты у сортов снизилось содержание линолевой кислоты. В целом сравнение метаболомных спектров зерновок диких видов и сортов позволило сделать следующие основные выводы. Ряд дикорастущих видов овса может быть рекомендован в качестве потенциального источника биохимических признаков качества при селекции. Выявлены метаболиты, содержание которых меняется в процессе окультуривания или по которым дикие виды овса отличаются от сортов этой культуры. Среди этих соединений наряду с такими широко известными составляющими здорового питания, как олеиновая кислота, глюкоза, фруктоза и др., выявлены моноацилглицеролы – МАГ 16 : 0, МАГ-2 18 : 2 и др. Высказано предположение, что последние могут быть связаны с формированием признаков адаптивности, в частности с устойчивостью к болезням и вредителям, абиотическим факторам среды.

Seed metabolomic profiles have been investigated in wild and cultivated forms (cultivars) of oat (Avena L.). Seed accessions from the VIR oat collection were used for the research. Metabolomic analysis employed gas liquid chromatography-mass spectrometry (GLC- MS) using an Agilent 6850 chromatographer (USA). The analysis covered the composition and content of organic and fatty acids, amino acids, polyatomic spirits and sugars. The content fluctuation range for the studied groups of compounds was found to be narrower (significantly in some cases) in cultivars than in the wild species. Along with a sharp increase in oleic acid content, cultivars demonstrated a decrease in that of linoleic acid. The general conclusions from the comparison of seed metabolomic profiles in wild species and cultivars are presented below. A number of wild species can be recommended as a potential source of biochemical quality traits for breeding purposes. A series of metabolites (compounds), the content of which changes during domestication or which differentiate wild oat species from cultivars has been identified was found. Along with such well-known healthy food chemical factors as oleic acid, glucose and fructose, etc., differences concerning monoacylglycerol compounds (MAG 16 : 0 and MAG-2 18 : 2, etc.) have been found. The latter have been proposed to be related to the formation of adaptive traits, in particular, resistance to diseases and pests, and to environmental abiotic stresses.

Avena L.дикие видысортазерновкигазовая хроматографиямасс-спектрометрияметаболомикаокультуриваниеадаптивность и полиморфизм признаковAvena L.wild speciesvarietiesseedsgas chromatographymass spectrometrymetabolomicsameliorationadaptability and polymorphism of characters.ReferencesAlpat’eva N.V., Gavrilyuk I.P., Leont’eva N.A., Oreshko L.S., Krasil’nikov V.N., Barsukova N.A., Loskutov I.G. Prolamins and celiac disease. Agrarnaya Rossiya = Agricultural Russia. 2004;6:41-49. (in Russian)Alpat’eva N.V., Gavrilyuk I.P., Leont’eva N.A., Oreshko L.S., Krasil’nikov V.N., Barsukova N.A., Loskutov I.G. Prolamins and celiac disease. Agrarnaya Rossiya = Agricultural Russia. 2004;6:41-49. (in Russian)Alpat’eva N.V., Gubareva N.K. Analysis of biotype composition in soft wheat landraces from the VIR collection in the course of storage and reproduction. Agrarnaya Rossiya = Agricultural Russia. 2002;3: 28-31. (in Russian)Alpat’eva N.V., Gubareva N.K. Analysis of biotype composition in soft wheat landraces from the VIR collection in the course of storage and reproduction. Agrarnaya Rossiya = Agricultural Russia. 2002;3: 28-31. (in Russian)Ermakov A.I. (Ed.) Metody biokhimicheskogo issledovaniya rasteniy. [Methods of biochemical evaluation of plants]. Leningrad, Agropromizdat Publ., 1987. (in Russian)Ermakov A.I. (Ed.) Metody biokhimicheskogo issledovaniya rasteniy. [Methods of biochemical evaluation of plants]. Leningrad, Agropromizdat Publ., 1987. (in Russian)Ermakov A.I., Ikonnikova M.I., Lukovnikova G.I., Yarosh N.P. The results and prospects of biochemical research of cultivated plants. Trudy po prikladnoy botanike, genetike i selektsii = Proceedings on Applied Botany, Genetics, and Breeding. 1969;41:326-364. (in Russian)Ermakov A.I., Ikonnikova M.I., Lukovnikova G.I., Yarosh N.P. The results and prospects of biochemical research of cultivated plants. Trudy po prikladnoy botanike, genetike i selektsii = Proceedings on Applied Botany, Genetics, and Breeding. 1969;41:326-364. (in Russian)Gu J., Jing L., Ma X., Zhang Z., Guo Q., Li Y. GC–TOF-MS-based serum metabolomic investigations of naked oat bran supplementation in high-fat-diet-induced dyslipidemic rats. J. Nutritional Biochemistry. 2015;26:1509-1519.Gu J., Jing L., Ma X., Zhang Z., Guo Q., Li Y. GC–TOF-MS-based serum metabolomic investigations of naked oat bran supplementation in high-fat-diet-induced dyslipidemic rats. J. Nutritional Biochemistry. 2015;26:1509-1519.Konarev A.V. Use of molecular markers in solving problems of genetic resources and breeding. Agrarnaya Rossiya = Agricultural Russia. 2006;6:4-23. (in Russian)Konarev A.V. Use of molecular markers in solving problems of genetic resources and breeding. Agrarnaya Rossiya = Agricultural Russia. 2006;6:4-23. (in Russian)Konarev A.V., Khoreva V.I. Biokhimicheskie issledovaniya geneticheskikh resursov rasteniy v VIRe [Biochemical research of plant genetic resources in VIR]. Saint-Petersburg, VIR Publ., 2000. (in Russian)Konarev A.V., Khoreva V.I. Biokhimicheskie issledovaniya geneticheskikh resursov rasteniy v VIRe [Biochemical research of plant genetic resources in VIR]. Saint-Petersburg, VIR Publ., 2000. (in Russian)Konarev A.V., Shelenga T.V., Perchuk I.N., Blinova E.V., Loskutov I.G. Characterization of diversity among oat (genus Avena L.) accessions from the VIR collection, an initial material for breeding Fusariumresistant oat varieties. Agrarnaya Rossiya = Agricultural Russia. 2015;5:2-10. (in Russian)Konarev A.V., Shelenga T.V., Perchuk I.N., Blinova E.V., Loskutov I.G. Characterization of diversity among oat (genus Avena L.) accessions from the VIR collection, an initial material for breeding Fusariumresistant oat varieties. Agrarnaya Rossiya = Agricultural Russia. 2015;5:2-10. (in Russian)Konarev A.V., Yarosh N.P. Nikolai Nikolaevich Ivanov, the founder of the Russian biochemistry of cultivated plants (120th birthday anniversary). Agrarnaya Rossiya = Agricultural Russia. 2005;2:3-4. (in Russian)Konarev A.V., Yarosh N.P. Nikolai Nikolaevich Ivanov, the founder of the Russian biochemistry of cultivated plants (120th birthday anniversary). Agrarnaya Rossiya = Agricultural Russia. 2005;2:3-4. (in Russian)Krasil’nikov V.N. Current directions of using plant genetic resources in technology for functional and special foods. Agrarnaya Rossiya = Agricultural Russia. 2015;11:36-42. (in Russian)Krasil’nikov V.N. Current directions of using plant genetic resources in technology for functional and special foods. Agrarnaya Rossiya = Agricultural Russia. 2015;11:36-42. (in Russian)Leonova S., Shelenga T., Hamberg M., Konarev A., Loskutov I., Carlsson A. Analysis of oil composition in cultivars and wild species of Oat (Avena sp.). J. Agric. Food Chemistry. 2008;56:7983-7991.Leonova S., Shelenga T., Hamberg M., Konarev A., Loskutov I., Carlsson A. Analysis of oil composition in cultivars and wild species of Oat (Avena sp.). J. Agric. Food Chemistry. 2008;56:7983-7991.Lokhov P.G., Archakov A.I. Mass-spectrometry methods in metabolomics. Biomeditsinskaya khimiya = Biomedical Chemistry. 2008;54: 497-511. (in Russian)Lokhov P.G., Archakov A.I. Mass-spectrometry methods in metabolomics. Biomeditsinskaya khimiya = Biomedical Chemistry. 2008;54: 497-511. (in Russian)Loskutov I.G. Oves (Avena L.). Rasprostranenie, sistematika, evolyutsiya i selektsionnaya tsennost [Oat (Avena L.): Distribution, taxonomy, evolution and breeding value]. Saint- Petersburg, VIR Publ., 2007. (in Russian)Loskutov I.G. Oves (Avena L.). Rasprostranenie, sistematika, evolyutsiya i selektsionnaya tsennost [Oat (Avena L.): Distribution, taxonomy, evolution and breeding value]. Saint- Petersburg, VIR Publ., 2007. (in Russian)Loskutov I.G., Gubareva N.K., Alpat’eva N.V. Polymorphism of avenin in the evaluation of wild oat species. Agrarnaya Rossiya = Agricultural Russia. 2005;2:43-48. (in Russian)Loskutov I.G., Gubareva N.K., Alpat’eva N.V. Polymorphism of avenin in the evaluation of wild oat species. Agrarnaya Rossiya = Agricultural Russia. 2005;2:43-48. (in Russian)Loskutov I.G., Rines H.W. Avena L. (Ed. C. Kole). Wild Crop Relatives: Genomic & Breeding Resources. Vol. 1. Cereals. Springer, Heidelberg, Berlin, New York, 2011;109-184.Loskutov I.G., Rines H.W. Avena L. (Ed. C. Kole). Wild Crop Relatives: Genomic & Breeding Resources. Vol. 1. Cereals. Springer, Heidelberg, Berlin, New York, 2011;109-184.Peneva T.I., Konarev V.G., Kobylyansky V.D., Lapikov N.S. Analysis of polymorphism for secalin spectrum in the development of winter rye cultivar Ilmen. Selskokhozyaystvennaya Biologiya = Agricultural Biology. 1998;1:55-62. (in Russian)Peneva T.I., Konarev V.G., Kobylyansky V.D., Lapikov N.S. Analysis of polymorphism for secalin spectrum in the development of winter rye cultivar Ilmen. Selskokhozyaystvennaya Biologiya = Agricultural Biology. 1998;1:55-62. (in Russian)Perkowski J., Stuper K., Buśko M., Góral T., Kaczmarek A., Jeleń H. Differences in metabolomic profiles of the naturally contaminated grain of barley, oats and rye. J. Cereal Sci. 2012;56:544-551.Perkowski J., Stuper K., Buśko M., Góral T., Kaczmarek A., Jeleń H. Differences in metabolomic profiles of the naturally contaminated grain of barley, oats and rye. J. Cereal Sci. 2012;56:544-551.Sánchez-Martín J., Heald J., Kingston-Smith A., Winters A., Rubiales D., Sanz M., Mur L. A. J., Prats E. A metabolomic study in oats (Avena sativa) highlights a drought tolerance mechanism based upon salicylate signalling pathways and the modulation of carbon, antioxidant and photo-oxidative metabolism. Plant, Cell & Environment. 2015;38:1434- 1452.Sánchez-Martín J., Heald J., Kingston-Smith A., Winters A., Rubiales D., Sanz M., Mur L. A. J., Prats E. A metabolomic study in oats (Avena sativa) highlights a drought tolerance mechanism based upon salicylate signalling pathways and the modulation of carbon, antioxidant and photo-oxidative metabolism. Plant, Cell & Environment. 2015;38:1434- 1452.Shelenga T.V., Konarev A.V., Dzubenko N.I., Malyshev L.L., Takai T. Evaluation of accessions of meadow fescue from the Vavilov Institute of Plant Industry containing symbiotic endophytic fungus of genus Neotyphodium. Doklady Rossiyskoy Akademii Selskokhozyaystvennykh Nauk = Proceedings of the Russian Academy of Agricultural Sciences. 2006;1:20-22. (in Russian)Shelenga T.V., Konarev A.V., Dzubenko N.I., Malyshev L.L., Takai T. Evaluation of accessions of meadow fescue from the Vavilov Institute of Plant Industry containing symbiotic endophytic fungus of genus Neotyphodium. Doklady Rossiyskoy Akademii Selskokhozyaystvennykh Nauk = Proceedings of the Russian Academy of Agricultural Sciences. 2006;1:20-22. (in Russian)Shelenga T.V., Solov’eva A.E., Shavarda A.L., Konarev A.V. Research of the metabolomes of crops of the VIR collection. Tezisy dokladov mezhdunarodnoy nauchnoy konferentsii, posvyashchennoy 120-letiyu VIR [Abstracts of Intern. sci. conference dedicated to the 120th anniversary of VIR, October 6-8, 2014]. Saint-Petersburg, 2014:98. (in Russian)Shelenga T.V., Solov’eva A.E., Shavarda A.L., Konarev A.V. Research of the metabolomes of crops of the VIR collection. Tezisy dokladov mezhdunarodnoy nauchnoy konferentsii, posvyashchennoy 120-letiyu VIR [Abstracts of Intern. sci. conference dedicated to the 120th anniversary of VIR, October 6-8, 2014]. Saint-Petersburg, 2014:98. (in Russian)Shulaev V., Cortes D., Miller G., Mittler R. Metabolomics for plant stress response. Physiologia Рlantarum. 2008;132:199-208.Shulaev V., Cortes D., Miller G., Mittler R. Metabolomics for plant stress response. Physiologia Рlantarum. 2008;132:199-208.Sitkin S.I., Tkachenko E.I., Vakhitov T.Ya., Oreshko L.S., Zhigalova T.N. Metabolome of blood serum as analyzed by gas chromatography– mass spectrometry (GC–MS) in patients with ulcerative colitis and celiac disease. Eksperimentalnaya i klinicheskaya gastroenterologiya = Experimental and Clinical Gastroenterology. 2013; 12:44-57. (in Russian)Sitkin S.I., Tkachenko E.I., Vakhitov T.Ya., Oreshko L.S., Zhigalova T.N. Metabolome of blood serum as analyzed by gas chromatography– mass spectrometry (GC–MS) in patients with ulcerative colitis and celiac disease. Eksperimentalnaya i klinicheskaya gastroenterologiya = Experimental and Clinical Gastroenterology. 2013; 12:44-57. (in Russian)Smolikova G.N., Shavarda A.L., Alekseichuk I.V., Chantseva V.V., Medvedev S.S. The metabolomic approach to the assessment of cultivar specificity of Brassica napus L. seeds. Russ. J. Genet.: Appl. Res. 2016;1:78-83.Smolikova G.N., Shavarda A.L., Alekseichuk I.V., Chantseva V.V., Medvedev S.S. The metabolomic approach to the assessment of cultivar specificity of Brassica napus L. seeds. Russ. J. Genet.: Appl. Res. 2016;1:78-83.Zelenskaya E.G., Konarev A.V., Loskutov I.G., Gubareva N.K., Strel’chenko P.P. Characterization of avenin polymorphism in oat (Avena sativa L.) landraces from the VIR collection. Agrarnaya Rossiya = Agricultural Russia. 2004;6:50-58. (in Russian)Zelenskaya E.G., Konarev A.V., Loskutov I.G., Gubareva N.K., Strel’chenko P.P. Characterization of avenin polymorphism in oat (Avena sativa L.) landraces from the VIR collection. Agrarnaya Rossiya = Agricultural Russia. 2004;6:50-58. (in Russian)

The authors declare that there are no conflicts of interest present.