vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ16.186vavilov-811Research ArticleХромосомная инженерия и отдаленная гибридизацияChromosome engineering and remote hybridizationСоздание новой серии анеуплоидных линий у хлопчатника (Gossypium hirsutum L.) с идентификацией отдельных хромосом с помощью транслокационных и SSR-маркеровThe creation of new aneuploid lines of the cotton (Gossypium hirsutum L.) with identification of chromosomes by translocation and SSR-markersСанамьянМ. Ф.SanamyanM. F.

Ташкент, Узбекистан

Tashkent, Uzbekistan

БобохужаевШ. У.BobokhujaevSh. U.

Ташкент, Узбекистан

Tashkent, Uzbekistan

МакамовА. Х.MakamovA. X.

Ташкент, Узбекистан

Tashkent, Uzbekistan

АчиловС. Г.AchilovS. G.

Ташкент, Узбекистан

Tashkent, Uzbekistan

АбдурахмоновИ. Ю.AbdurakhmonovI. Y.

Ташкент, Узбекистан

Tashkent, Uzbekistan

Национальный университет Узбекистана им. М. УлугбекаУзбекистанNational University of Uzbekistan named M. UlugbekUzbekistanЦентр геномики и биоинформатики Академии наук Республики УзбекистанУзбекистанCenter of Genomics and Bioinformatics UASUzbekistan201630122016205643652Copyright © Санамьян М.Ф., Бобохужаев Ш.У., Макамов А.Х., Ачилов С.Г., Абдурахмонов И.Ю., 20162016Санамьян М.Ф., Бобохужаев Ш.У., Макамов А.Х., Ачилов С.Г., Абдурахмонов И.Ю.Sanamyan M.F., Bobokhujaev S.U., Makamov A.X., Achilov S.G., Abdurakhmonov I.Y.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/811

Использование моносомных линий значительно увеличивает эффективность молекулярно-генетического анализа и получения высококачественных селекционных линий с замещением определенных хромосом. В связи с этим было важно создать полный набор анеуплоидных линий для каждой хромосомы хлопчатника. Достижению этой цели способствовало получение новых моно-сомных линий хлопчатника узбекской коллекции с использованием хромосом-специфичных маркеров (SSR) и транслокационных линий для идентификации хромосом. В статье обобщены данные многолетних исследований, посвященных созданию моносомных линий, включая происхождение 95 первичных моносомиков хлопчатника, их цитогенетические и морфологические особенности, а также идентификацию унивалентных хромосом с помощью транслокационного теста и SSR-маркеров. Линии идентифицированы по хромосомам 2, 4, 6 Аt-субгенома и хромосомам 18, 22 Dt-субгенома, а также выявлен телоцентрик для 11 хромосомы Аt-субгенома хлопчатника. Остальные 22 моносомные линии были идентифицированы как дубликаты трех хромосом (2, 4, 6). Созданный материал будет полезен для молекулярно-генетического картирования, создания линий с замещением хромосом и для селекции хлопчатника.

The use of monosomic lines significantly increases the effectiveness of molecular-genetic analysis and the development of superior quality breeding lines via substitutions by alien chromosomes. A complete set of aneuploid series for each cotton chromosome was required. Simple sequence repeats (SSR) have been applied as useful markers for understanding cotton genetics. Several DNA markers have already been assigned to the individual chromosomes of Gossypium hirsutum. The primary objective of this paper is to report the creation of new monosomic lines and the use of chromosome specific simple sequence repeat (SSR) markers and translocation lines for chromosome identification to confirm chromosome specificity of monosomic lines in the Uzbek collection. Here, we summarize data on the development of a monosomic stock collection of cotton (G. hirsutum L.) from Uzbekistan, including the origin of 95 primary monosomics, their cytogenetic characteristics, morphological features and their identification by translocation tests and chromosome SSR-markers. Our results indicated several different monosomic lines identified by chromosomes 2, 4, 6 and telosome 11 of the At subgenome and chromosomes 18 and 22 of the Dt subgenome, 22 monosomes were identified as duplicates of three monosomes (chromosomes 2, 4 and 6). Chromosome 4 of the At subgenome was recovered more frequently (18 times) than chromosome 2 of the At subgenome (4 times) and chromosome 6 of the At subgenome (3 times) during pollen irradiation at different doses and in the progeny of the desynaptic plants. These lines will be useful for molecular mapping and enhancement of Upland cotton.

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The authors declare that there are no conflicts of interest present.