vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ16.208vavilov-867Research ArticleЭкологическая генетикаEcological geneticsСимбиотическая бактерия Wolbachia в популяциях вредителя хвойных лесов Dendrolimus superans sibiricus Tschetverikov, 1908 (Lepidoptera: Lasiocampidae)Wolbachia infection in populations of the coniferous forest pest Dendrolimus superans sibiricus Tschetverikov, 1908 (Lepidoptera: Lasiocampidae)ЮдинаМ. А.YudinaM. A.

Новосибирск, Россия

Novosibirsk, Russia

ДубатоловВ. В.DubatolovV. V.

Новосибирск, Россия

Novosibirsk, Russia

БыковР. А.BykovR. A.

Новосибирск, Россия

Novosibirsk, Russia

ИлинскийЮ. Ю.IlinskyYu. Yu.

Новосибирск, Россия

Novosibirsk, Russia

paulee@bionet.nsc.ruФедеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук» Федеральное государственное автономное образовательное учреждение высшего образования «Новосибирский национальный исследовательский государственный университет»РоссияInstitute of Cytology and Genetics SB RAS Novosibirsk State UniversityRussian FederationФедеральное государственное бюджетное учреждение науки Институт систематики и экологии животных Сибирского отделения Российской академии наукРоссияInstitute of Systematics and Ecology of Animals SB RASRussian FederationФедеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук»РоссияInstitute of Cytology and Genetics SB RASRussian Federation201602022017206899903Copyright © Юдина М.А., Дубатолов В.В., Быков Р.А., Илинский Ю.Ю., 20172017Юдина М.А., Дубатолов В.В., Быков Р.А., Илинский Ю.Ю.Yudina M.A., Dubatolov V.V., Bykov R.A., Ilinsky Y.Y.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/867

Сибирский шелкопряд – крайне опасный вредитель хвойных деревьев, в особенности лиственницы и различных видов сосен. Вспышки численности сибирского шелкопряда приводят к дефолиации и гибели лесов на обширных площадях в азиатской части Российской Федерации. Многие биологические агенты, такие как вирусы, патогенные микроорганизмы и паразитоиды, сдерживают рост популяции вредителя. В данной работе рассмотрен непатогенный симбиотический микроорганизм Wolbachia, который передается от особи к особи трансовариально и может оказывать серьезное влияние на биологию вида-хозяина. Потенциально Wolbachia может как сдерживать, так и стимулировать рост популяции вредителя, что и определяет ключевой интерес исследования. Две выборки сибирского шелкопряда, собранные в 2014 и 2016 гг. в Хабаровском крае, были исследованы на присутствие Wolbachia. Обнаружена высокая частота инфицированных особей сибирского шелкопряда. Доля носителей эндосимбионта в 2014 г. составила 100 % и в 2016 г. – 90 %. Кроме того, в исследованной популяции сибирского шелкопряда на основании протокола мультилокусного генотипирования по генам f tsZ и f bpA обнаружено присутствие по меньшей мере двух штаммов Wolbachia, характеризующихся аллельными вариантами f tsZ-36, f bpA-4 и f tsZ-22, f bpA-9. В статье обсуждается возможная роль Wolbachia в симбиотической ассоциации с сибирским шелкопрядом и направления дальнейших исследований.

Siberian silk moth (Dendrolimus superans sibiricus) is a very dangerous pest of coniferous trees, in particular, larch and various pine species. Outbreaks of this pest lead to defoliation and forest destruction in a vast area of the Asian part of Russia. Many biological agents, such as viruses, pathogenic microorganisms and parasitoids, prevent the growth of Siberian silk moth population. Here we consider non-pathogen symbiotic Wolbachia bacteria, which are transovarially transmitted between specimens from mother to offspring. This symbiont has an ability to affect biology of its host. In theory, Wolbachia can prevent the growth of population size or induce it, which determines the focus of interest in Wolbachia-host investigation. Two samples from a Siberian silk moth population collected in 2014 and 2016 in Khabarovsk area were studied for Wolbachia infection. We found a high Wolbachia prevalence in the population of Siberian silk moth, in particular, the sample of 2014 was totally infected and the sample of 2016 had 90 % infected specimens. There were at least two distinct Wolbachia strains reveled by analysis of two loci from the MLST protocol, namely f tsZ-36, f bpA-4 and f tsZ-22, f bpA-9. In this study, a possible role of Wolbachia in the symbiotic association with Siberian silk moth and general ways of investigation of this symbiosis are discussed.

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The authors declare that there are no conflicts of interest present.