vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ17.222vavilov-903Research ArticleГенофонд и селекцияGene pool and breedingSvSv-линии – эффективный инструмент для вовлечения в селекцию ценного генофонда 1 EBN диплоидных диких видов картофеляSvSv-lines is an effective tool for involvement of the valuable genepool of 1 EBN diploid potato species into breedingЕрмишинА. П.YermishinA. P.

Минск

Minsk

ПолюховичЮ. В.PolyukhovichYu. V.

Минск

Minsk

ВоронковаЕ. В.VoronkovaE. V.

Минск

Minsk

ГукасянО. Н.GukasyanO. N.

Минск

Minsk

Государственное научное учреждение «Институт генетики и цитологии НАН Беларуси»БеларусьInstitute of Genetics and Cytology of NAS of BelarusBelarus2017240320172114250Copyright © Ермишин А.П., Полюхович Ю.В., Воронкова Е.В., Гукасян О.Н., 20172017Ермишин А.П., Полюхович Ю.В., Воронкова Е.В., Гукасян О.Н.Yermishin A.P., Polyukhovich Y.V., Voronkova E.V., Gukasyan O.N.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/903

Имеется ряд ценных для селекции диких видов картофеля, в частности 1 EBN мексиканские диплоидные виды, которые практиче- ски не скрещиваются с культурным картофелем, включая дигаплоиды Solanum tuberosum (2х, 2 EBN), из-за жестких пре- и постзиготных межвидовых репродуктивных барьеров. Для вовлечения в селекцию генофонда этих видов предлагается использовать оригинальные SvSv-линии (F2 дигаплоиды S. tuberosum×S. verrucosum), у которых St -аллели культурного картофеля замещены на Sv от самосовместимого диплоидного вида S. verrucosum, не образующего пестичных S-РНКаз. Предполагалось, что благодаря наличию в гомозиготном состоянии S-генов, привнесенных от S. verrucosum, SvSv-линии имеют те же возможности для устранения презиготной несовместимости при межвидовой гибридизации, что и этот дикий вид, однако их использование позволит устранить нежелательные эффекты, связанные с его применением (мужская стерильность гибридов, пониженное клубнеобразование). Целью исследования была оценка эффективности использования SvSv-линий (по сравнению с S. verrucosum) в качестве посредника для вовлечения в селекцию 1 EBN видов картофеля S. bulbocastanum, S. pinnatisectum и S. polyadenium. Установлено, что SvSv-линии превосходят S. verrucosum по эффективности гибридизации с 1 EBN видами благодаря обильному и продолжительному цветению в более поздние, по сравнению с S. verrucosum, сроки, когда наступает цветение видов-опылителей. Среди гибридов между SvSv-линиями и 1 EBN дикими видами было достоверно меньше стерильных и низкофертильных генотипов и больше генотипов со средней и высокой функциональной фертильностью пыльцы. Они обладали более высокой способностью к клубнеобразованию, благодаря чему, в отличие от гибридов на основе S. verrucosum, были пригодны для многолетней селекционной оценки в полевых условиях. Полученные межвидовые гибриды удалось скрестить в качестве материнских форм с дигаплоидами S. tuberosum.

There is a set of wild potato species valuable for breeding, in particular 1 EBN diploid species from Mexico, which is practically not crossable to cultivated potatoes, including Solanum tuberosum dihaploids (2х, 2 EBN), because of strict pre- and postzygotic interspecific reproductive barriers. It is proposed to involve that species germplasm into breeding by using the original SvSv-lines (F2 S. tuberosum dihaploids×S. verrucosum), in which St -alleles have been substituted for Sv from the self-compatible diploid species S. verrucosum not producing stylar S-RNase. It was anticipated that SvSv-lines have the same ability for elimination of prezygotic incompatibility in interspecific hybridization as S. verrucosum due to S-genes of that species in homozygous state. However, their use would help avoid undesirable effects associated with S. verrucosum application (male sterility and reduced tuber formation in hybrids). The aim of the research was estimation of the efficiency of using SvSv-lines, in comparison with S. verrucosum, as a bridge for involvement of the 1 EBN potato species S. bulbocastanum, S. pinnatisectum and S. polyadenium into breeding. It was revealed that SvSv-lines excels S. verrucosum in hybridization efficacy due to abundant and prolonged flowering in later term than S. verrucosum, when flowering of pollinator species occurs. There were significantly less sterile or low fertility genotypes among the hybrids between SvSv-lines and 1 EBN wild species. They had higher ability for tuber formation, and so they were suitable for long term field trials, contrast to hybrids with S. verrucosum. The interspecific hybrids produced were crossed as female parents to S. tuberosum dihaploids.

картофельотдаленная гибридизацияпрезиготная несовместимостьбалансовое число эндосперма – EBNS. verrucosumSvSv-линииpotatohybridization between distantly related speciesprezygotic incompatibilityendosperm balance number – EBNS. verrucosumSvSv-linesReferencesAbdalla M.M., Hermsen J.G.Th. Plasmons and male sterility types in Solanum verrucosum and its interspecific hybrid derivatives. Euphytica. 1972;21:209-220.Abdalla M.M., Hermsen J.G.Th. Plasmons and male sterility types in Solanum verrucosum and its interspecific hybrid derivatives. Euphytica. 1972;21:209-220.Abdalla M.M., Hermsen J.G.Th. An evaluation of Solanum verrucosum Schlechtd. for its possible use in potato breeding. Euphytica. 1973;22:19-27.Abdalla M.M., Hermsen J.G.Th. An evaluation of Solanum verrucosum Schlechtd. for its possible use in potato breeding. Euphytica. 1973;22:19-27.Bamberg J.B. A practical method for making wild potato germplasm from Mexico accessible for evaluation and utilization. Am. Potato J. 1990;67:539.Bamberg J.B. A practical method for making wild potato germplasm from Mexico accessible for evaluation and utilization. Am. Potato J. 1990;67:539.Cardi T., D’Ambrosio F., Consoli D., Puite K.J., Ramulu K.S. Production of somatic hybrids between frost resistant Solanum commersonii and S. tuberosum. Theor. Appl. Genet. 1993;87:193-200.Cardi T., D’Ambrosio F., Consoli D., Puite K.J., Ramulu K.S. Production of somatic hybrids between frost resistant Solanum commersonii and S. tuberosum. Theor. Appl. Genet. 1993;87:193-200.Carputo D., Barone A., Cardi T., Sebastiano A., Frusciante L., Peloquin S.J. Endosperm Balance Number manipulation for direct in vivo germplasm introgression to potato from a sexually isolated relative (Solanum commersonii Dun.). Proc. Natl. Acad. Sci. USA. 1997;94(22):12013-12017.Carputo D., Barone A., Cardi T., Sebastiano A., Frusciante L., Peloquin S.J. Endosperm Balance Number manipulation for direct in vivo germplasm introgression to potato from a sexually isolated relative (Solanum commersonii Dun.). Proc. Natl. Acad. Sci. USA. 1997;94(22):12013-12017.Carputo D., Monti L., Werner J.E., Frusciante L. Uses and usefulness of endosperm balance number. Theor. Appl. Genet. 1999;98:478-484.Carputo D., Monti L., Werner J.E., Frusciante L. Uses and usefulness of endosperm balance number. Theor. Appl. Genet. 1999;98:478-484.Dinu I.I., Hayes R.J., Kynast R.G., Phillips R.L., Thill C.A. Novel inter-series hybrids in Solanum, section Petota. Theor. Appl. Genet. 2005;110(3):403-415.Dinu I.I., Hayes R.J., Kynast R.G., Phillips R.L., Thill C.A. Novel inter-series hybrids in Solanum, section Petota. Theor. Appl. Genet. 2005;110(3):403-415.Eijlander R. Mechanisms of self-incompatibility and unilateral incompatibility in diploid potato (Solanum tuberosum L.). NL: Wageningen Agricult. Univ., 1998.Eijlander R. Mechanisms of self-incompatibility and unilateral incompatibility in diploid potato (Solanum tuberosum L.). NL: Wageningen Agricult. Univ., 1998.Hanneman R.E., Jr. The reproductive biology of the potato and its implication for breeding. Potato Res. 1999;42:283-312.Hanneman R.E., Jr. The reproductive biology of the potato and its implication for breeding. Potato Res. 1999;42:283-312.Hawkes J.C. The Potato. Evolution, Biodiversity and Genetic Resources. London: Belhaven Press, 1990.Hawkes J.C. The Potato. Evolution, Biodiversity and Genetic Resources. London: Belhaven Press, 1990.Helgeson J.P., Pohlman J.D., Austin S., Haberlach G.T., Wielgus S.M., Ronis D., Zambolin L., Tooley P., McGrath J.M., James R.V., Stevenson W.R. Somatic hybrids between Solanum bulbocastanum and potato: a new source of resistance to late blight. Theor. Appl. Genet. 1998;96:738-742.Helgeson J.P., Pohlman J.D., Austin S., Haberlach G.T., Wielgus S.M., Ronis D., Zambolin L., Tooley P., McGrath J.M., James R.V., Stevenson W.R. Somatic hybrids between Solanum bulbocastanum and potato: a new source of resistance to late blight. Theor. Appl. Genet. 1998;96:738-742.Hermsen J.G.Th., Ramanna M.S. Double-bridge hybrids of Solanum bulbocastanum and cultivars of Solanum tuberosum. Euphytica. 1973;22:457-466.Hermsen J.G.Th., Ramanna M.S. Double-bridge hybrids of Solanum bulbocastanum and cultivars of Solanum tuberosum. Euphytica. 1973;22:457-466.Hermsen J.G.Th., Ramanna M.S. Barriers to hybridization of Solanum bulbocastanum Dun. and Solanum verrucosum Schlechtd. and structural hybridity in their F1 plants. Euphytica. 1976;25:1-10.Hermsen J.G.Th., Ramanna M.S. Barriers to hybridization of Solanum bulbocastanum Dun. and Solanum verrucosum Schlechtd. and structural hybridity in their F1 plants. Euphytica. 1976;25:1-10.Jansky S.H., Hamernik A.J. The introgression of 2x 1 EBN Solanum species into the cultivated potato using Solanum verrucosum as a bridge. Genet. Resour. Crop Evol. 2009;56:1107-1115.Jansky S.H., Hamernik A.J. The introgression of 2x 1 EBN Solanum species into the cultivated potato using Solanum verrucosum as a bridge. Genet. Resour. Crop Evol. 2009;56:1107-1115.Jansky S.H., Peloquin S.J., Yerk G.L. Use of potato haploids to put 2x wild species germplasm in usable form. Plant Breeding. 1990;104:290-294.Jansky S.H., Peloquin S.J., Yerk G.L. Use of potato haploids to put 2x wild species germplasm in usable form. Plant Breeding. 1990;104:290-294.Johnston S.A., Hanneman R.E., Jr. Support of the endosperm balance number hypothesis utilizing some tuber-bearing Solanum species. Am. Potato J. 1980;57(1):7-14.Johnston S.A., Hanneman R.E., Jr. Support of the endosperm balance number hypothesis utilizing some tuber-bearing Solanum species. Am. Potato J. 1980;57(1):7-14.Johnston S.A., Hanneman R.E., Jr. Manipulations of endosperm balance number overcome crossing barriers between diploid Solanum species. Science. 1982;217:446-448.Johnston S.A., Hanneman R.E., Jr. Manipulations of endosperm balance number overcome crossing barriers between diploid Solanum species. Science. 1982;217:446-448.Kameraz A.Ya., Vavilova M.A., Zhitlova N.A. Interspecific hybridization of potatoes with participation of Mexican wild diploid species S. verrucosum Schlechtd. Trudy po prikladnoy botanike, genetike i selektsii = Proceedings on Applied Botany, Genetics, and Breeding. 1982;73:56-65. (in Russian)Kameraz A.Ya., Vavilova M.A., Zhitlova N.A. Interspecific hybridization of potatoes with participation of Mexican wild diploid species S. verrucosum Schlechtd. Trudy po prikladnoy botanike, genetike i selektsii = Proceedings on Applied Botany, Genetics, and Breeding. 1982;73:56-65. (in Russian)Luksha V.I., Savchuk A.V., Voronkova E.V., Yermishin A.P. Results of selection on character “high functional pollen fertility” and a set of breeding indexes in populations of Solanum tuberosum L. dihaploids. Kartofelevodstvo = Potato Growing (Minsk). 2010;17:137-148. (in Russian)Luksha V.I., Savchuk A.V., Voronkova E.V., Yermishin A.P. Results of selection on character “high functional pollen fertility” and a set of breeding indexes in populations of Solanum tuberosum L. dihaploids. Kartofelevodstvo = Potato Growing (Minsk). 2010;17:137-148. (in Russian)Makhan’ko O.V. Interspecific incompatibility in potato diploid breeding. Zemlyarobstva i akhova raslin=Agriculture and Plant Protection. 2008;1:11-14. (in Russian)Makhan’ko O.V. Interspecific incompatibility in potato diploid breeding. Zemlyarobstva i akhova raslin=Agriculture and Plant Protection. 2008;1:11-14. (in Russian)Nyman M., Waara S. Characterization of somatic hybrids between Solanum tuberosum and its frost-tolerant relative Solanum commersonii. Theor. Appl. Genet. 1997;95:1127-1132.Nyman M., Waara S. Characterization of somatic hybrids between Solanum tuberosum and its frost-tolerant relative Solanum commersonii. Theor. Appl. Genet. 1997;95:1127-1132.Pallais N., Fong N., Berrios D. Research on the physiology of potato sexual seed production. Innovative Methods for Propagating Potatoes. CIP Rep. 28th Planning Conf. CIP, Lima, 1984;149-168.Pallais N., Fong N., Berrios D. Research on the physiology of potato sexual seed production. Innovative Methods for Propagating Potatoes. CIP Rep. 28th Planning Conf. CIP, Lima, 1984;149-168.Pankin A., Sokolova E., Rogozina E., Kuznetsova M., Deal K., Jones R., Khavkin E. Allele mining in the gene pool of wild Solanum species for homologues of late blight resistance gene RB/Rpi-blb1. Plant Genet. Resources. 2011;9(2):305-308.Pankin A., Sokolova E., Rogozina E., Kuznetsova M., Deal K., Jones R., Khavkin E. Allele mining in the gene pool of wild Solanum species for homologues of late blight resistance gene RB/Rpi-blb1. Plant Genet. Resources. 2011;9(2):305-308.Polyukhovich Yu.V., Makhan’ko O.V., Savchuk A.V., Voronkova E.V., Yermishin A.P. Development of bridge lines for overcoming interspecific incompatibility in potatoes. Vestsi NAN Belarusi. Ser. biyal. navuk = Proc. Nat. Acad. Sci. Belarus. Ser. Biol. Sci. 2010;2:51-58. (in Russian)Polyukhovich Yu.V., Makhan’ko O.V., Savchuk A.V., Voronkova E.V., Yermishin A.P. Development of bridge lines for overcoming interspecific incompatibility in potatoes. Vestsi NAN Belarusi. Ser. biyal. navuk = Proc. Nat. Acad. Sci. Belarus. Ser. Biol. Sci. 2010;2:51-58. (in Russian)Polyukhovich Yu.V., Savchuk A.V., Voronkova E.V., Yermishin A.P. The efficacy of hybridization between Solanum verrucosum Schldl and 1 EBN wild diploid potato species with use of rescue pollination by S. phureja IvP 35. Doklady NAN Belarusi = Reports Nat. Acad. Sci. Belarus. 2011;55(3):88-92. (in Russian)Polyukhovich Yu.V., Savchuk A.V., Voronkova E.V., Yermishin A.P. The efficacy of hybridization between Solanum verrucosum Schldl and 1 EBN wild diploid potato species with use of rescue pollination by S. phureja IvP 35. Doklady NAN Belarusi = Reports Nat. Acad. Sci. Belarus. 2011;55(3):88-92. (in Russian)Polyukhovich Yu.V., Voronkova E.V., Savchuk A.V., YermishinA.P. Use of Solanum verrucosum for overcoming unilateral incompatibility in crosses with allotetraploid wild potato species. Kartofelevodstvo = Potato Growing (Minsk). 2013;21(1):136-145. (in Russian)Polyukhovich Yu.V., Voronkova E.V., Savchuk A.V., YermishinA.P. Use of Solanum verrucosum for overcoming unilateral incompatibility in crosses with allotetraploid wild potato species. Kartofelevodstvo = Potato Growing (Minsk). 2013;21(1):136-145. (in Russian)Sokolova E.A., Fadina O.V., Khavkin E.E. Metodicheskie ukazaniya: molekulyarnye markery genov ustoychivosti i genomov-donorov ustoychivosti kartofelya k fitoftorozu [Methodology guidelines: molecular markers of resistance genes and genomes that are donors of resistance of potatoes to late blight]. Мoscow, All-Russia Research Institute of Agricultural Biotechnology Publ., 2013. (in Russian)Sokolova E.A., Fadina O.V., Khavkin E.E. Metodicheskie ukazaniya: molekulyarnye markery genov ustoychivosti i genomov-donorov ustoychivosti kartofelya k fitoftorozu [Methodology guidelines: molecular markers of resistance genes and genomes that are donors of resistance of potatoes to late blight]. Мoscow, All-Russia Research Institute of Agricultural Biotechnology Publ., 2013. (in Russian)Thieme R., Darsow U., Gavrilenko T., Dorokhov D., Tiemann H. Production of somatic hybrids between S. tuberosum L. and late blight resistant Mexican wild potato species. Euphytica. 1997;97:189-200.Thieme R., Darsow U., Gavrilenko T., Dorokhov D., Tiemann H. Production of somatic hybrids between S. tuberosum L. and late blight resistant Mexican wild potato species. Euphytica. 1997;97:189-200.Voronkova E.V., Polyukhovich Yu.V., Savchuk A.V., Gukasyan O.N., Yermishin A.P. Field resistance to late blight of hybrids produced by pollination of bridge species Solanum verrucosum and SvSv-lines derived from it by diploid 1 EBN potato species. Molekulyarnaya i prikladnaya genetika = Molecular and Applied Genetics (Minsk). 2013;15:104-110. (in Russian)Voronkova E.V., Polyukhovich Yu.V., Savchuk A.V., Gukasyan O.N., Yermishin A.P. Field resistance to late blight of hybrids produced by pollination of bridge species Solanum verrucosum and SvSv-lines derived from it by diploid 1 EBN potato species. Molekulyarnaya i prikladnaya genetika = Molecular and Applied Genetics (Minsk). 2013;15:104-110. (in Russian)Yermishin A.P. Incompatibility in interspecific and intraspecific hybridization of diploid potatoes and the approaches to its overcoming. Vestsi NAN Belarusi. Ser. biyal. navuk = Proc. Nat. Acad. Sci. Belarus. Ser. Biol. Sci. 2001;3:105-118. (in Russian)Yermishin A.P. Incompatibility in interspecific and intraspecific hybridization of diploid potatoes and the approaches to its overcoming. Vestsi NAN Belarusi. Ser. biyal. navuk = Proc. Nat. Acad. Sci. Belarus. Ser. Biol. Sci. 2001;3:105-118. (in Russian)Yermishin A.P., Makhan’ko O.V., Voronkova E.V. Application of somatic hybrids between dihaploids of potato Solanum tuberosum L. and wild diploid species from Mexico in breeding: generation and backcrossing of dihaploids of somatic hybrids. Genetika = Genetics (Moscow). 2006;42(12):1414-1421. (in Russian)Yermishin A.P., Makhan’ko O.V., Voronkova E.V. Application of somatic hybrids between dihaploids of potato Solanum tuberosum L. and wild diploid species from Mexico in breeding: generation and backcrossing of dihaploids of somatic hybrids. Genetika = Genetics (Moscow). 2006;42(12):1414-1421. (in Russian)Yermishin A.P., Polyukhovich Yu.V., Savchuk A.V., Voronkova E.V. A new look at the problem of inter-EBN interspecific crosses in potato. EAPR 2011: Abstracts of the 18th Triennial Conf. of the Eur. Assoc. for Potato Research, July 24–29, 2011, Oulu, Finland. Eds. J. Santala, J.P.T. Valkonen. Helsinki, Finland, 2011;71.Yermishin A.P., Polyukhovich Yu.V., Savchuk A.V., Voronkova E.V. A new look at the problem of inter-EBN interspecific crosses in potato. EAPR 2011: Abstracts of the 18th Triennial Conf. of the Eur. Assoc. for Potato Research, July 24–29, 2011, Oulu, Finland. Eds. J. Santala, J.P.T. Valkonen. Helsinki, Finland, 2011;71.Yermishin A.P., Polyukhovich Y.V., Voronkova E.V., Savchuk A.V. Production of hybrids between 2 EBN bridge species Solanum verrucosum and 1 EBN diploid potato species. Am. J. Potato Res. 2014;91:610-617. DOI 10/1007/s12230-014-9385-9.Yermishin A.P., Polyukhovich Y.V., Voronkova E.V., Savchuk A.V. Production of hybrids between 2 EBN bridge species Solanum verrucosum and 1 EBN diploid potato species. Am. J. Potato Res. 2014;91:610-617. DOI 10/1007/s12230-014-9385-9.Yermishin A.P., Teltsova Yu.V., Savchuk A.V., Makhan’ko O.V. Development of the bridge lines with the sc-system from Solanum verrucosum for overcoming interspecific incompatibility in potatoes. Materialy mezhdunarodnoi konferentsii “Otdalennaya gibridizatsiya. Sovremennoe sostoyanie i perspektivy razvitiya” [Proc. Intern. Conf. “Distant hybridization: State of the art and prospects of development”, Moscow 16–17 December 2003]. M.: MSKhA Publ., 2003;96-100. (in Russian)Yermishin A.P., Teltsova Yu.V., Savchuk A.V., Makhan’ko O.V. Development of the bridge lines with the sc-system from Solanum verrucosum for overcoming interspecific incompatibility in potatoes. Materialy mezhdunarodnoi konferentsii “Otdalennaya gibridizatsiya. Sovremennoe sostoyanie i perspektivy razvitiya” [Proc. Intern. Conf. “Distant hybridization: State of the art and prospects of development”, Moscow 16–17 December 2003]. M.: MSKhA Publ., 2003;96-100. (in Russian)

The authors declare that there are no conflicts of interest present.