vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ17.241vavilov-935Research ArticleГЕНЕТИКА РАСТЕНИЙPLANT GENETICSПолимерное взаимодействие генов SHY2 и MSG1, NPH4 и IAR2 при наследовании признаков корневой системы Arabidopsis thaliana (L.) Heynh.Polymer interaction of the genes SHY2 and MSG1, NPH4 and IAR2 in the inheritance of the Arabidopsis thaliana (L.) Heynh. root systemХаблакС. Г.HablakS. G.

Харьков

Kharkov

serhab211981@yandex.uaХарьковский национальный аграрный университет им. В.В. ДокучаеваУкраинаV.V. Dokuchaev Kharkov National Agrarian UniversityUkraine201722042017212227233Copyright © Хаблак С.Г., 20172017Хаблак С.Г.Hablak S.G.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/935

Регуляция ветвления корней – важный адаптивный механизм, обеспечивающий приспособление растений к среде обитания корней. Выяснение генетических механизмов, вызывающих у растений увеличение степени ветвления корней, имеет существенное значение в повышении отзывчивости сельскохозяйственных культур на элементы питания.

Целью исследования было изучение взаимодействия генов SHY2 и MSG1, NPH4 и IAR2 при наследовании признаков корневой системы Arabidopsis thaliana. При скрещивании растений мутантных линий shy2-2×msg1-2, nph4-1×iar2-1 в F2 получено расщепление, позволяющее предполагать полимерное взаимодействие генов SHY2 и MSG1, NPH4 и IAR2. Расщепление по фенотипу в поколении F2 происходит в соотношении 15:1. Описанные в работе результаты исследований представляют интерес для практического использования хозяйственно ценного признака «ветвление корней» в селекции растений для создания сортов и гибридов с заданными свойствами минерального питания. В данной работе показано, что способность растений увеличивать степень ветвления корней зависит от отдельных генов и может наследоваться по типу полимерного взаимодействия генов. Зная полимерный характер наследования в корневой системе длины боковых корней при взаимодействии генов, можно комбинировать гены путем скрещивания и увеличивать степень ветвления корней у культурных растений при создании сортов и гибридов, более отзывчивых на элементы питания. 

Roots branching regulation is an important adaptive mechanism for the adaptation of plants to root environments. Elucidation of the genetic mechanisms involved in increase in the degree of plant root branching is essential in improving the responsiveness of crops to supply elements. The aim was to study the interaction of the genes SHY2 and MSG1, NPH4 and IAR2 as attributes of the root system of A. thaliana are inherited. By crossing plants of the mutant lines shy2-2×msg1-2, nph4-1×iar2-1, a segregation in F2 was observed, suggesting an interaction between the polymer SHY2 and MSG1, NPH4 and IAR2 genes. The segregation ratio of the phenotypes in F2 is 15:1. The results presented are of interest for practical use of the economically valuable trait «branching roots» in plant breeding to create varieties and hybrids with the desired properties of mineral nutrition. Our data indicate that the ability of plant roots to increase the degree of branching depends on individual genes and can be inherited through polymer gene interactions. Knowing the polymeric nature of inheritance in the root system, the length of lateral roots in the interaction of genes can be combined by crossing genes and increase the degree of branching of the roots from cultivated plants to create agrochemically effective varieties and hybrids.

Arabidopsis thaliana (L.) Heynh.корневая системагенмутациявзаимодействие геновArabidopsis thaliana (L.) Heynh.root systemgenemutationgene interactionReferencesAbel S., Nguyen D., Theologis A. The PS-lAA415-like family of early auxin-inducible mRNAs in Arabidopsis thaliana. J. Mo1. Biol. 1995;251(2):533-549.Abel S., Nguyen D., Theologis A. The PS-lAA415-like family of early auxin-inducible mRNAs in Arabidopsis thaliana. J. Mo1. Biol. 1995;251(2):533-549.Blakesley D., Weston G.D., Hall J.F. The role of endogenous auxin in root initiation. Plant Growth Regul. 1991;10(1):341-353.Blakesley D., Weston G.D., Hall J.F. The role of endogenous auxin in root initiation. Plant Growth Regul. 1991;10(1):341-353.Borovikov V. STATISTICA. Iskusstvo analiza dannykh na komp’yutere: dlya professionalov [STATISTICA. Art of the computer analysis of data: for experts]. S.-Petersburg, Piter Publ., 2003. (in Russian)Borovikov V. STATISTICA. Iskusstvo analiza dannykh na komp’yutere: dlya professionalov [STATISTICA. Art of the computer analysis of data: for experts]. S.-Petersburg, Piter Publ., 2003. (in Russian)Braaksma F.J., Feenstra W.J. Nitrate reduction in Аrabidopsis thaliana. Arabid. Inf. Serv. 1975;12(1):16-17.Braaksma F.J., Feenstra W.J. Nitrate reduction in Аrabidopsis thaliana. Arabid. Inf. Serv. 1975;12(1):16-17.Doddema H., Hofstra J.J., Feenstra W.J. Uptake of nitrate by mutants of Arabidopsis thaliana, disturbed in uptake or reduction of nitrate. Physiol. Plantarum. 1978;43(4):343-350.Doddema H., Hofstra J.J., Feenstra W.J. Uptake of nitrate by mutants of Arabidopsis thaliana, disturbed in uptake or reduction of nitrate. Physiol. Plantarum. 1978;43(4):343-350.Glazko V.I., Glazko G.V. Slovar’ terminov po prikladnoy genetike i DNK tekhnologiyam [Glossary of terms in applied genetics and DNA technology]. Kiev, Nora-Print Publ., 1999. (in Russian)Glazko V.I., Glazko G.V. Slovar’ terminov po prikladnoy genetike i DNK tekhnologiyam [Glossary of terms in applied genetics and DNA technology]. Kiev, Nora-Print Publ., 1999. (in Russian)Gorodniy N.G., Ustimenko A.S., Danilchuk P.V. Kornevye sistemy i produktivnost’ sel’skokhozyaystvennykh rasteniy [The root systems and productivity of agricultural crops]. Urozhay Publ., 1975. (in Russian)Gorodniy N.G., Ustimenko A.S., Danilchuk P.V. Kornevye sistemy i produktivnost’ sel’skokhozyaystvennykh rasteniy [The root systems and productivity of agricultural crops]. Urozhay Publ., 1975. (in Russian)Harper R.M., Stowe-Evans E.L., Luesse D.R., Muto H., Tatematsu K., Watahiki M.K., Yamamoto K., Liscum E. The NPH4 locus encodes the auxin response factor ARF7, a conditional regulator of differential growth in aerial Arabidopsis tissue. Plant Cell. 2000;12(4): 757-770.Harper R.M., Stowe-Evans E.L., Luesse D.R., Muto H., Tatematsu K., Watahiki M.K., Yamamoto K., Liscum E. The NPH4 locus encodes the auxin response factor ARF7, a conditional regulator of differential growth in aerial Arabidopsis tissue. Plant Cell. 2000;12(4): 757-770.Khablak S.G., Abdullaeva J.A. Effect of auxin-induced genes on root branching in Arabidopsis thaliana (L.) Heynh. Vestn. Khar’kovskogo natsional’nogo agrarnogo universiteta. Ser. Biologiya = Bulletin of the Kharkov National Agrarian University. Biology Series. 2012;1(25):57-63. (in Russian)Khablak S.G., Abdullaeva J.A. Effect of auxin-induced genes on root branching in Arabidopsis thaliana (L.) Heynh. Vestn. Khar’kovskogo natsional’nogo agrarnogo universiteta. Ser. Biologiya = Bulletin of the Kharkov National Agrarian University. Biology Series. 2012;1(25):57-63. (in Russian)Khablak S.G., Pariy F.N. The relationship between the signaling system regulating plant development and gene interaction in the inheritance of root system traits in Arabidopsis thaliana (L.) Heynh. Vestn. Khar’kovskogo natsional’nogo agrarnogo universiteta. Ser. Biologiya = Bulletin of the Kharkov National Agrarian University. Biology Series. 2013;3(30):83-89. (in Russian)Khablak S.G., Pariy F.N. The relationship between the signaling system regulating plant development and gene interaction in the inheritance of root system traits in Arabidopsis thaliana (L.) Heynh. Vestn. Khar’kovskogo natsional’nogo agrarnogo universiteta. Ser. Biologiya = Bulletin of the Kharkov National Agrarian University. Biology Series. 2013;3(30):83-89. (in Russian)Kulaeva O.N. Dwarf mutants and their role in the “green revolution”. Soros Educational Journal. 2000;8:18-23. (in Russian)Kulaeva O.N. Dwarf mutants and their role in the “green revolution”. Soros Educational Journal. 2000;8:18-23. (in Russian)Lakin G.F. Biometriya [Biometrics]. Moscow, 1990. (in Russian)Lakin G.F. Biometriya [Biometrics]. Moscow, 1990. (in Russian)Medvedev S.S., Sharova E.I. Genetic and epigenetic regulation of plant organisms (review). J. Siberian Federal University Biology. 2010;2(3):109-129. (in Russian)Medvedev S.S., Sharova E.I. Genetic and epigenetic regulation of plant organisms (review). J. Siberian Federal University Biology. 2010;2(3):109-129. (in Russian)Novikovа G.V., Stepanchenko N.S., Nosov A.V., Moshkov I.E. At the beginning: the perception of ABA and the transduction of its signal in plants. Fiziologiya rasteniy = Plant Physiology (Moscow). 2009;56(6):806-823. (in Russian)Novikovа G.V., Stepanchenko N.S., Nosov A.V., Moshkov I.E. At the beginning: the perception of ABA and the transduction of its signal in plants. Fiziologiya rasteniy = Plant Physiology (Moscow). 2009;56(6):806-823. (in Russian)Robinson D. Resource capture by localized root proliferation: Why do plants bother? Ann. Bot. 1996;77(2):179-185.Robinson D. Resource capture by localized root proliferation: Why do plants bother? Ann. Bot. 1996;77(2):179-185.Romanov G.A. How cytokinins act on the cell. Fiziologiya rasteniy = Plant Physiology (Moscow). 2009;56(2):295-319. (in Russian)Romanov G.A. How cytokinins act on the cell. Fiziologiya rasteniy = Plant Physiology (Moscow). 2009;56(2):295-319. (in Russian)Rubinа B.A., Chernavina I.A., Potapov N.G. Bol’shoy praktikum po fiziologii rasteniy [Major training course on plant physiology]. Moscow, 1978. (in Russian)Rubinа B.A., Chernavina I.A., Potapov N.G. Bol’shoy praktikum po fiziologii rasteniy [Major training course on plant physiology]. Moscow, 1978. (in Russian)Seed List. The Nottingham Arabidopsis Stock Centre. Nottingham: The University of Nottingham, 1994.Seed List. The Nottingham Arabidopsis Stock Centre. Nottingham: The University of Nottingham, 1994.Shpakov A.O. Chemosignaling systems in plants. Tsitologiya = Cytology. 2009;51(9):721-733. (in Russian)Shpakov A.O. Chemosignaling systems in plants. Tsitologiya = Cytology. 2009;51(9):721-733. (in Russian)Taranovskaya M.G. Metody izucheniya kornevykh sistem [Methods of studying root systems]. Moscow, Selkhozizdat Publ., 1957. (in Russian)Taranovskaya M.G. Metody izucheniya kornevykh sistem [Methods of studying root systems]. Moscow, Selkhozizdat Publ., 1957. (in Russian)Tarchevskiy I.A. Signal’nye sistemy kletok rasteniy [Signalling systems of plant cells]. Moscow, Nauka Publ., 2002. (in Russian)Tarchevskiy I.A. Signal’nye sistemy kletok rasteniy [Signalling systems of plant cells]. Moscow, Nauka Publ., 2002. (in Russian)Tian Q., Uhlir N.J., Reed J.W. Arabidopsis SHY2/IAA3 inhibits auxinregulated gene expression. Plant Cell. 2002;14(2):301-319.Tian Q., Uhlir N.J., Reed J.W. Arabidopsis SHY2/IAA3 inhibits auxinregulated gene expression. Plant Cell. 2002;14(2):301-319.Tsygankova V.A., Galkina L.A., Musatenko L.I., Sytnik K.M. Genetic and epigenetic control of plant growth and development. The genes of the biosynthesis of auxins and auxin-regulated genes that control cell division and elongation of plant cells. Biopolimery i kletka = Biopolymers and the Cell. 2005;21(2):107-133. (in Russian)Tsygankova V.A., Galkina L.A., Musatenko L.I., Sytnik K.M. Genetic and epigenetic control of plant growth and development. The genes of the biosynthesis of auxins and auxin-regulated genes that control cell division and elongation of plant cells. Biopolimery i kletka = Biopolymers and the Cell. 2005;21(2):107-133. (in Russian)Walker L., Estelle M. Molecular mechanisms of auxin action. Plant Biol. 1998;1(2):434-439.Walker L., Estelle M. Molecular mechanisms of auxin action. Plant Biol. 1998;1(2):434-439.Wilmoth J.C., Wang S., Tiwari S.B. NPH4/ARF7 and ARF19 promote leaf expansion and auxin-induced lateral root formation. Plant J. 2005;43(1):118-130.Wilmoth J.C., Wang S., Tiwari S.B. NPH4/ARF7 and ARF19 promote leaf expansion and auxin-induced lateral root formation. Plant J. 2005;43(1):118-130.

The authors declare that there are no conflicts of interest present.