vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ17.255vavilov-969Research ArticleФилогенетикаPhylogeneticsГенетическое разнообразие видов комплекса Aporrectodea caliginosa на территории РоссииGenetic diversity of the Aporrectodea caliginosa complex in RussiaШеховцовС. В.ShekhovtsovS. V.

Новосибирск

Novosibirsk

shekhovtsov@bionet.nsc.ruГоловановаЕ. В.GolovanovaE. V.

Омск

Omsk

БазароваН. Э.BazarovaN. E.

Новосибирск

Novosibirsk

БеловаЮ. Н.BelovaYu. N.

Вологда

Vologda

БерманД. И.BermanD. I.

Магадан

Magadan

ДержинскийЕ. А.DerzhinskyE. A.

Витебск

Vitebsk

ШашковМ. П.ShashkovM. P.

Пущино

Pushchino

ПельтекС. Е.PeltekS. E.

Новосибирск

Федеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук»РоссияInstitute of Cytology and Genetics SB RASRussian FederationФедеральное государственное бюджетное образовательное учреждение высшего образования «Омский государственный педагогический университет»РоссияOmsk State Pedagogical UniversityRussian FederationФедеральное государственное бюджетное образовательное учреждение высшего образования «Вологодский государственный университет»РоссияVologda State UniversityRussian FederationФедеральное государственное бюджетное учреждение науки Институт биологических проблем Севера Дальневосточного отделения Российской академии наукРоссияInstitute of Biological Problems of the North FEB RASRussian FederationУчреждение образования «Витебский государственный университет им. П.М. Машерова»БеларусьP.M. Masherov Vitebsk State UniversityBelarusФедеральное государственное бюджетное учреждение науки Институт физико-химических и биологических проблем почвоведения Российской академии наукРоссияnstitute of Physicochemical and Biological Problems of Soil Sciences RASRussian FederationФедеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук»РоссияInstitute of Cytology and Genetics SB RAS, NovosibirskRussian Federation201724052017213374379Copyright © Шеховцов С.В., Голованова Е.В., Базарова Н.Э., Белова Ю.Н., Берман Д.И., Держинский Е.А., Шашков М.П., Пельтек С.Е., 20172017Шеховцов С.В., Голованова Е.В., Базарова Н.Э., Белова Ю.Н., Берман Д.И., Держинский Е.А., Шашков М.П., Пельтек С.Е.Shekhovtsov S.V., Golovanova E.V., Bazarova N.E., Belova Y.N., Berman D.I., Derzhinsky E.A., Shashkov M.P., Peltek S.E.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/969

Дождевые черви комплекса Aporrectodea caliginosa – обычные обитатели многих антропогенных и естественных биотопов, нередко доминанты сообществ. Считается, что на территории России встречаются два подвида: A. c. caliginosa и A. c. trapezoides. В последнее время к этому комплексу относят и вид Aporrectodea longa. В статье сделана попытка обобщения полученных материалов о генетическом разнообразии названных таксонов червей, собранных нами в России, а также в некоторых точках Беларуси и Казахстана. Подвид A. c. caliginosa на территории России представлен двумя филогенетическими линиями – второй и третьей, при этом последняя доминирует (около 73 % образцов). Кроме того, линии имеют различную распространенность: вторая обнаружена практически на всей исследуемой территории, третья встречалась в небольшом количестве точек на периферии обследованной территории. Генетическая изменчивость червей второй линии заметно выше, чем третьей, а время дивергенции почти в три раза больше. Часть образцов, определенных по степени пигментации как A. c. caliginosa, имела гаплотип cox1 A. c. trapezoides; результаты анализа ядерных последовательностей подтвердили диагноз. Таким образом, степень пигментации у названного подвида может значительно варьировать. Кроме того, два образца A. longa имели последовательности, идентичные последовательностям филогенетической линии 1 этого вида, известной с севера Западной Европы. Это первые случаи выявления в Западной Сибири A. c. trapezoides и A. longa. Приведенные данные свидетельствуют о том, что в ряду A. c. caliginosa – линия 2 – A. c. caliginosa – линия 3 – A. c. trapezoides и A. longa наблюдается снижение и генетической изменчивости и встречаемости.

Earthworms of the Aporrectodea caliginosa species complex are abundant in many anthropogenic and natural habitats and often predominate in earthworm communities. In Russia, there are two subspecies of the complex, A. c. caliginosa and A. c. trapezoides; Aporrectodea longa was also recently mentioned as a putative member of the complex. In this study, we made an attempt to review available data on the species complex studied based on our collection from Russia, Belarus, and Kazakhstan. The subspecies A. c. caliginosa is represented in Russia by two genetic lineages, 2 and 3, the former being the prevalent (about 73 % of the total sample). Additionally, these lineages have different distributions: while lineage 2 was found in almost all locations studied, lineage 3 was detected only in a few samples from the periphery of the region studied. The genetic diversity of lineage 2 significantly exceeded that of lineage 3, and its estimated divergence time was almost three times as high. A subset of individuals with pigmentation characteristic of A. c. caliginosa contained cox1 haplotypes of A. c. trapezoides; analysis of nuclear gene sequences confirmed this diagnosis. Thus, pigmentation intensity in this subspecies was demonstrated to vary to a significant degree. In addition, we analyzed two A. longa individuals from West Siberia and the Urals; their cox1 sequences were identical to those from the lineage 1 of this species from the north of Western Europe. These are the first reports of A. c. trapezoides and A. longa from West Siberia. On the whole, both genetic diversity and abundance was shown to decrease in the following series: A. c. caliginosa lineage 2 – A. c. caliginosa lineage 3 – A. c. trapezoides and A. longa.

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The authors declare that there are no conflicts of interest present.