Evaluation of wheat products with high flavonoid content: justification of importance of marker-assisted development and production of flavonoid-rich wheat cultivars

In the structure of the global commodity supply in the food market in modern conditions it is necessary to note the emergence of a broad group of new high-tech products, and specialized functional food with high value added. The creation of varieties with a high content of flavonoids (plant compounds that can have a positive effect on human health) is one of the important directions of plant breeding oriented on the functional foods development. Currently, however, there is a significant gap between the well-studied role of flavonoids and the genetic control of their synthesis, on the one hand, and development of the actual product of wheat with new properties, evaluation of the nutritional value of the end-use bakery products for consumption, on the other hand. In the present study we produced and investigated bakery products from wheat synthesizing bioflavonoid pigments anthocyanins in the grain pericarp. The grains of this wheat have dark purple color. Red-grained bread wheat was a control. These two wheat lines have almost similar genomes with the exception of a small part of chromosome 2A, which contains the Pp3/TaMyc1 gene regulating anthocyanin biosynthesis. The use of such an accurate model has allowed relating the observed differences precisely with anthocyanin biosynthesis. The important task was to evaluate the resistance of anthocyanins to the backing process. Therefore anthocyanin content was evaluated not only in the end-use product, but also in mixtures of flour and bran used for baking and separately in the bran. As a result, significant differences were detected in samples obtained from purple grains, compared with the control including the products that had passed a full processing cycle, including baking at elevated temperature. For the extraction of anthocyanins conditions were simulated most similar to those in the process of digestion in the stomach, in order to assess the amount of assimilable anthocyanins. By our estimates one can get up to 1.03 mg of assimilable anthocyanins with 100 g of whole-grained bread produced from anthocyanincolored grains. With 100 g of bran, the body will get up to 3.32 g of anthocyanins. In parallel with the evaluation of the anthocyanins content in all samples, the mass fraction of antioxidants was measured by using the amperometric method. The highest antioxidant capacity was shown for bran, while the least one was demonstrated for flour. Adding bran to the flour as well as the backing process increased the antioxidant capacity of wheat products. The contribution of anthocyanins to increased antioxidant capacity is not significant. It was shown that bread-making quality and organoleptic properties of bakery products made from anthocyanin-colored grains did not concede, or in some cases were higher than the corresponding properties of products obtained from control NIL grains. It was found that the presence of anthocyanin increases the shelf life of bakery products and their resistance to molding in provocative conditions. These results, combined with the known data about the beneficial health effects of anthocyanins, suggest that wheat bakery products made from anthocyanin-rich grains can be included to the list for dietary food. Marker-assisted selection accelerating the creation of new forms of crops with a high level of flavonoids can be proposed as a new direction for the expansion of domestic and export grain market potential due to the new possibilities of obtaining products of increased nutritional value and making a good profit.

anthocyanins, bioflavonoids, domestic and export potential, genes, DNA markers, grain, marker-assisted selection, common wheat, bread, baking properties, nutritional value, functional food

1. Abdel-Aal E.S., Hucl P. A rapid method for quantifying total anthocyanins in blue aleurone and purple pericarp wheats. Cereal Chem. 1999;76(3):350-354. DOI 10.1094/CCHEM.1999.76.3.350.

2. Adzhieva V.F., Babak O.G., Shoeva O.Y., Kilchevsky A.V., Khlestkina E.K. Molecular genetic mechanisms of the development of fruit and seed coloration in plants. Russ. J. Genet. Appl. Res. 2016;6(5): 537-552. DOI 10.1134/S2079059716050026.

3. Aggarwal B.B., Shishodia S. Molecular targets of dietary agents for prevention and therapy of cancer. Biochem. Pharmacol. 2006;71(10): 1397-1421. DOI 10.1016/j.bcp.2006.02.009.

4. Bruschi V., Teuber R., Dolgopolova I. Acceptance and willingness to pay for health-enhancing bakery products – Empirical evidence for young urban Russian consumers. Food Quality and Preference. 2015;46:79-91. DOI 10.1016/j.foodqual.2015.07.008.

5. Cassidy A., O’Reilly E.J., Kay C., Sampson L., Franz M., Forman J.P., Curhan G., Rimm E.B. Habitual intake of flavonoid subclasses and incident hypertension in adults. Am. J. Clin. Nutr. 2011;93(2):338- 347. DOI 10.3945/ajcn.110.006783.

6. de Pascual-Teresa S., Moreno D.A., Garcia-Viguera C. Flavanols and anthocyanins in cardiovascular health: a review of current evidence. Int. J. Mol. Sci. 2010;11(4):1679-1703. DOI 10.3390/ijms11041679.

7. Ficco D.B.M., de Simone V., Colecchia S.A., Pecorella I., Platani C., Nigro F., Finocchiaro F., Papa R., de Vita P. Genetic variability in anthocyanin composition and nutritional properties of blue, purple, and red bread (Triticum aestivum L.) and durum (Triticum turgidum L. ssp. turgidum convar. durum) wheats. J. Agric. Food Chem. 2014;62(34):8686-8695. DOI 10.1021/jf5003683.

8. Gordeeva E.I., Shoeva O.Y., Khlestkina E.K. Marker-assisted development of bread wheat near-isogenic lines carrying various combinations of Pp (purple pericarp) alleles. Euphytica. 2015;203:469-476. DOI 10.1007/s10681-014-1317-8.

9. GOST R 52189-2003. Muka pshenichnaya. Obshchie tekhnicheskie usloviya [State Standard R 52189-2003. Flour. General specifications]. Moscow: Standartinform Publ., 2008. (in Russian)

10. GOST R 52349-2005. Produkty pishchevye. Produkty pishchevye funktsionalnye. Terminy i opredeleniya [State Standart R 52349-2005. Foodstuffs. Functional foods. Terms and definitions]. Moscow: Standartinform Publ., 2008. (in Russian)

11. GOST 27676-88. Zerno i produkty ego pererabotki. Metod opredeleniya chisla padeniya [State Standart 27676-88. Cereals and cereal milled products. Method for determination of falling number]. Moscow: Standartinform Publ., 2009. (in Russian)

12. GOST 27839-2013. Muka pshenichnaya. Metody opredeleniya kolichestva i kachestva kleykoviny [State Standart 27839-2013. Wheat flour. Methods of determination of gluten quantity and quality]. Moscow: Standartinform Publ., 2014. (in Russian)

13. Howard B.V., Kritchevsky D. Phytochemicals and cardiovascular disease a statement for healthcare professionals from the American heart association. Circulation. 1997;95(11):2591-2593. DOI 10.1161/01.CIR.95.11.2591.

14. Ivanišová E., Ondrejovič M., Chmelova D., Maliar T., Havrlentova M., Rückschloss L. Antioxidant activity and polyphenol content in milling fractions of purple wheat. Cereal Res. Commun. 2014;42(4):578- 588. DOI 10.1556/CRC.2014.0008.

15. Khlestkina E.K., Pshenichnikova T.A., Usenko N.I., Otmakhova Yu.S. Prospective applications of molecular genetic approaches to control technological properties of wheat grain in the context of the “grain–flour–bread” chain. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2016;20(4):511- 527. DOI 10.18699/VJ15.140. (in Russian)

16. Khlestkina E.K., Shoeva O.Y., Gordeeva E.I. Flavonoid biosynthesis genes in wheat. Russ. J. Genet. Appl. Res. 2015;5(3):268-278. DOI 10.1134/S2079059715030077.

17. Lila M.A. Anthocyanins and human health: an in vitro investigative approach. J. Biomed. Biotechnol. 2004;2004(5):306-313. DOI 10.1155/ S111072430440401X.

18. Nile S.H., Park S.W. Edible berries: Bioactive components and their effect on human health. Nutrition. 2014;30(2):134-144. DOI 10.1016/ j.nut.2013.04.007.

19. Puchkova L.I. Laboratornyy praktikum po tekhnologii khlebopekarnogo proizvodstva [Laboratory manual on baking technology]. St. Petersburg: GIORD Publ., 2004. (in Russian)

20. Romanov A.S., Davydenko N.A., Shatnyuk L.N., Matveeva I.V., Poznyakovsky V.M. Ekspertiza khleba i khlebobulochnykh izdeliy. Kachestvo i bezopasnost: uchebnoe posobie [Examination of bread and bakery products: quality and safety, a textbook]. Ed. by V.M. Poznyakovsky. Saratov: Vuzovskoe obrazovanie Publ., 2014. (in Russian)

21. Rossiya prodaet bolshe zerna, chem oruzhiya (2015) [Russia sells more grains than weapons (2015)]. Available at: http://www.pravda. ru/economics/agriculture/farming/14-10-2015/1277717-grain-0/ (in Russian)

22. Rynok funktsional’nykh produktov pitaniya (2014) [The functional food market (2014)]. Available at http://sfera.fm/articles/rynokfunktsionalnykh-produktov-pitaniya (in Russian)

23. Shobana S., Sreerama Y.N., Malleshi N.G. Composition and enzyme inhibitory properties of finger millet (Eleusine coracana L.) seed coat phenolics: Mode of inhibition of α-glucosidase and pancreatic amylase. Food Chem. 2009;115(4):1268-1273. DOI 10.1016/j.foodchem. 2009.01.042.

24. Shoeva O.Y., Khlestkina E.K., Berges H., Salina E.A. The homoeologous genes encoding chalcone-flavanone isomerase in Triticum aestivum L.: Structural characterization and expression in different parts of wheat plant. Gene. 2014;538(2):334-341.

25. Tadera K., Minami Y., Takamatsu K., Matsuoka T. Inhibition of α-glucosidase and α-amylase by flavonoids. J. Nutr. Sci. Vitaminol. 2006;52(2):149-153. DOI 10.3177/jnsv.52.149.

26. Tereshchenko O., Gordeeva E., Arbuzova V., Börner A., Khlestkina E. The D genome carries a gene determining purple grain colour in wheat. Cereal Res. Commun. 2012;40(3):334-341. DOI 10.1556/ CRC.40.2012.3.2.

27. Tsuda T., Horio F., Uchida K., Aoki H., Osawa T. Dietary cyanidin 3-O-β-D-glucoside-rich purple corn color prevents obesity and ameliorates hyperglycemia in mice. J. Nutr. 2003;133(7):2125-2130.

28. Usenko N.I., Poznyakovsky V.M., Otmakhova Y.S. Structural and qualitative transformations in the bread market (analysis of the current state and development problems). EKO (Vserossiyskiy ekonomicheskiy zhurnal) = ECO Journal (Novosibirsk). 2016;1:109-124. (in Russian)

29. Virgili F., Marino M. Regulation of cellular signals from nutritional molecules: a specific role for phytochemicals, beyond antioxidant activity. Free Radic. Biol. Med. 2008;45(9):1205-1216. DOI 10.1016/j.freeradbiomed.2008.08.001.

30. Williams R.J., Spencer J.P., Rice-Evans C. Flavonoids: antioxidants or signalling molecules? Free Radic. Biol. Med. 2004;36(7):838-849. DOI 10.1016/j.freeradbiomed.2004.01.001.