STUDY OF INTROGRESSIVE LINES OF COMMON WHEAT WITH Aegilops tauschii GENETIC MATERIAL FOR RESISTANCE TO LEAF RUST

The synthetic forms of Triticum miguschovae (GGAADD), Triticum palmovae (A^bA^bDD), T. durum (M. it.)/Ae. Tauschii (BBAADD) and the introgressive lines of common wheat created on their basis were evaluated for resistance to leaf rust. All synthetic forms have a high resis­tance to leaf rust. Twenty-two lines of common wheatresistant to leaf rust with genetic material from T. miguschovae, 10 lines with genetic material from T. du­rum (M. it.)/Ae. tauschii and 4 lines obtained on the basis of T. palmovae were identified. A screening with the use of molecular markers for the presence of leaf rust resistance genes Lr21, Lr26, Lr32, Lr39 was done. The GDM35 marker linked to the Lr39 gene was identified in the synthetic forms. Molecular markers Lr21F/R and BARC135 linked to the genes Lr21 and Lr32, respectively, were not identified. Resistance to leaf rust in lines 729, 1555, 2203, 2289, 2295, 2296, 4155, 4171, obtained on the basis of T. miguschovae, lines 3261, 3265, obtained on the basis of T. palmovae, and in line 4141 with the genetic material from T. durum (M. it.)/Ae. Tauschii is controlled by the presence of the Lr39 gene. The SCM9 marker indicating the presence of translocation of 1BL.1RS with the Lr26 gene was detected in 15 lines obtained on the basis of T. miguschovae, in 2 lines with genetic material from T. durum (M. it.)/Ae. tauschii, and in 1 line created on the basis of T. palmovae. Lines 729, 1555, 2203, 2289, 2295, 2296, 4155, 4171 obtained with the participation of T. miguschovae and line 3261 with the participation of T. palmovae carry a combination of genes (Lr39+Lr26).

1. Davoyan R.O., Bebyakina I.V., Davoyan O.R., Zinchenko A.N., Davoyan E.R., Kravchenko A.M., Zubanova Y.S. Use of synthetic forms in the preservation and exploitation of the gene pool of wild common wheat relatives. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2012;16(1):44-51.

2. Gultyaeva E.I. Methods of identification of wheat leaf rust resistance genes with use of DNA-markers and characterization of efficiency of Lr-genes. St. Petersburg, 2012;59-60. (in Russian) Hiebert C.W., Thomas J.B., Somers D.J., McCallum B.D., Fox S.L.

3. Microsatellite mapping of adult-plant leaf rust resistance gene Lr22a in wheat. Theor. Appl. Genet. 2007;115:877-884.

4. Huang L., Gill B.S. An RGA-like marker detects all known Lr21 leaf rust-resistance gene family members in Aegilops tauschii and wheat. Theor. Appl. Genet. 2001;103(6-7):1007-1013.

5. Ivanov G.I. New amphidiploid of wheat with the genomic constitution DDAbAb. Nauchno-tekhnicheskiy byulleten VNII rastenievodstva = Scientific and Technological Bulletin of the Vavilov Institute of Plant Industry.1984;142:78-79. (in Russian)

6. Kim W., Jonson P.S., Baenziger P.S., Lukaszewski A.J., Gaines C.S. Agronomic effect of wheat-rye translocation carrying rye chromatin (1R) from different sources. Crop Sci. 2004;44:1254-1258.

7. Kovalenko E.D., Zhemchuzhina A.I., Kiseleva M.I., Kolomiets Т.М., Scherbik А.А. Strategy of wheat breeding for resistance to rust deseases. Zashchita i karantin rasteniy = Plant Protection and Quarantine. 2012;9:19-22. (in Russian)

8. Mains E.B., Jakson H.S. Рhysiologic specialization in leaf rust of wheat, Puccinia triticiana Erikss. Phytopathology. 1926;16:89-120.

9. McIntosh R.A., Yamayaki Y., Dubcovsky J., Rogers J., Morris C., Appels R., Xia X. Catalogue of Gene Symbols for Wheat: 2013 Supplement. Available at: http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2013.

10. Mesterhazy A., Bartos P., Goyeau G., Niks R. European survey for leaf rust in wheat. Agronomie. 2000;20:793-804.

11. Plaschke J., Ganal M.W., Röder M.S. Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor. Appl. Genet. 1995;91:1001-1007.

12. Sadovaya А.S., Gultyaeva E.I., Mitrofanova О.P., Shaidayuk Е.L., Hakimova А.G., Zuev Е.V. Leaf rust resistance in common wheat varieties and lines from the collection of the Vavilov plant industry institute carrying alien genetic material. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2014;18(4):739-750. (in Russian)

13. Singh S., Franks C.D., Huang L., Brown-Guedira G.L., Marshall D.S., Gill B.S., Fritz A. Lr41, Lr39 and a leaf rust resistance gene from Aegilops cylindrica may be allelic and are located of wheat chromosome 2DS. Theor. Appl. Genet. 2004;108:586-591.

14. Thomas J., Nilmalgoda S., Hiebert C., McCallum B., Humphreys G., DePauw R. Genetic markers and leaf rust resistance of the wheat gene Lr32. Crop Sci. 2010;50(6):2310-2317.

15. Weng Y., Azhaguvel P., Devkota R.N., Rudd J.C. PCR-based markers for detection of different sources 1AL.1RS and 1BL.1RS wheatrye translocations in wheat background. Plant Breed. 2007;126(5): 482-486.

16. Zhemchuzhina A., Kurkova N. Structure of population of Puccinia triticina in various regions of Russia in 2006–2008. Abstr. of the 8th Int. Wheat Conference, June 1–4, 2010, St. Petersburg, Russia. St. Petersburg, 2010;279.

17. Zhirov E.G., Ternovskaya T.K. Genomic engineering in wheat. Vestnik selskokhozyaystvennoy nauki = Herald of Agricultural Sciences. 1984;10:58-66. (in Russian)