A tendency towards leaf rust resistance decrease in common wheat introgression lines with genetic material from Aegilops speltoides Tausch

Leaf rust, caused by the fungus Puccinia triticina Erikss., inflicts serious crop loss of common wheat Triticum aesti­vum L. The species Aegilops speltoides Tausch (2n = 14, SS) is considered a promising issue of genes to protect bread wheat from diseases. The objective of this study was the monitoring of resistance to leaf rust of Ae. speltoides ac­cessions and introgressive lines and cultivars with genetic material of this species to the Western Siberian popula­tion of fungus. The estimation of specimens in the field conditions on natural infectious background showed that the Ae. speltoides accessions were immune to leaf rust, however, а tendency towards increasing susceptibility of the introgressive lines and varieties was detected. The protective effect of the known genes Lr28, Lr36 and Lr35 decreased, but Lr47 remained efficient in West Siberia, as confirmed by the results of testing of the Omsk population of P. triticina for virulence to the mentioned genes. During the study (2003-2017) the resistance has been overcome of lines Od 26/89, 156/90, analogs of cv. Novosibirskaya 67 – ANK-39 (B, C), and L-500 from the “Arsenal” сollection. High resistance to leaf rust was preserved of lines Od (35/1, 35/89, 210/90, 278/89); ANK39 (A, D, E); L-501 and cvs. Chelyaba 75 and Mit. Analysis of DNA markers and phytopathological tests showed that the studied variet­ies and lines lacked the known genes Lr28, Lr36, and Lr47 from Ae. speltoides (except for the lines of cvs. Thatcher and Pavon). On the base of analysis of DNA markers, it was assumed that Chelyaba 75 and seven resistant lines from the Odand ANK-39-series have translocations bearing the LrSp gene. Presumably, the rest of samples possess additional not yet identified genes of Ae. speltoides. The trend of overcoming of resistance to leaf rust of introgres­sive lines and varieties with Ae. speltoides genes should be taken into consideration in common wheat breeding in Western Siberia.

Triticum aestivum, Aegilops speltoides, introgres¬sive lines, Puccinia triticina, virulence, Lr-genes, molecular markers

1. Parlevliet J.E. Durability of resistance against fungal, bacterial and viral pathogens; present situation. Euphytica. 2002;124:147-156.

2. Adonina I.G., Leonova I.N., Badaeva E.D., Salina E.A. Genotyping of hexaploid wheat varieties from different Russian regions. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2016;20(1):44-50. DOI 10.18699/VJ16.107. (in Russian)

3. Peterson R.F., Campbell A.B., Hannah A.E. A diagrammatic scale for estimating rust intensity of leaves and stem of cereals. Can. J. Res. 1948;Sect. C. 26:496-500.

4. Anikster Y., Manisterski J., Long D.L., Leonard K.J. Resistance to leaf rust, stripe rust, and stem rust in Aegilops ssp. in Israel. Plant Dis. 2005;89:303-308. DOI 10.1094/PD-89-0303.

5. Plotnikova L.Ya., Kuzmina S.P. Genetic and physiological bases of resistance to leaf rust in introgressive lines of Novosibirskaya 67 variety with genes from Aegilops speltoides Tausch. Proceedings of the 2nd All-Russian (national) scientific conference “The Role of Agrarian Science in the Sustainable Development of Rural Areas”, Novosibirsk, December 25, 2017. Novosibirsk, 2017;100-102. (in Russian)

6. Davoyan E.R., Davoyan R.O., Bebyakina I.V., Davoyan O.R., Zubanova Yu.S., Zinchenko A.N., Kravchenko A.M. Identification of leaf-rust resistance genes in species of Aegilops L., synthetic forms, and introgression lines of common wheat. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2012;16(1):116-122. (in Russian)

7. Plotnikova L.Ya., Pozherukova V.E., Meshkova L.V., Mitrofanova O.P., Degtyarev A.I., Aidosova A.T. The resistance of Timofeevi wheat to Puccinia triticina in West Siberia. Mikologiya i Fitopatologiya = Mycology and Phytopathology. 2015;49(2):116-125. (in Russian)

8. Dorohov D.B., Cloke E. Rapid and economic technology of RAPD-analysis of plant genomes. Molekulyarnaya Genetika = Molecular Genetics. 1997;3(4):443-450. (in Russian)

9. Plotnikova L.Ya., Stubey T.Yu. Appearance of resistance to Puccinia triticina determined by the genes Lr13, Lr22b, and Lr35 in adult plants. Mikologiya i Fitopatologiya = Mycology and Phytopathology. 2009;43(3):258-271. (in Russian)

10. Esimbekova M.A., Bulatova K.M., Kushanova R.Zh., Mukin K.B. Biodeverssity of wild cereals of the Aegilops L. genius in Kazakhstan in the context of wheat breeding. Izvestiya Timiryazevskoy Selskokhozyaystvennoy Akademii = Izvestiya of Timiryazev Agricultural Academy. 2015;6:5-18. (in Russian)

11. Salina E.A., Adonina I.G., Badaeva E.D., Stasyuk A.I., Leonova I.N., Shishkina A.A., Divashuk M.G., Stankova E.V., Khuat T.M., Syukov V.V., Karlov G.I. A Thinopyrum intermedium chromosome in bread wheat cultivars as a source of genes conferring resistance to fungal diseases. Euphytica. 2015;204:91-101. DOI 10.1007/s10681014-1344-5.

12. Eversmeyer M.G., Kramer C.L. Epidemiology of wheat leaf and stem rust in the central great plains of the USA. Annu. Rev. Phytopathol. 2000;38:491-513.

13. Sanin S.S. Phytosanitary monitoring: current state and ways of improvement. Problems of Optimization of the Phytosanitary Condition of Plant Growing. Proceedings of the All-Russian Congress on Plant Protection, St. Petersburg, December 1995. St. Petersburg, 1997;166-176. (in Russian)

14. Friebe B., Jiang J., Raupp W.J., McIntosh R.A., Gill B.S. Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica. 1996;91:59-87.

15. Shah T.M., Ahmed J., Asghar M., Iqbal N., Farooq S. Evaluation of annual wild grass species for leaf rust resistance. Pak. J. Biol. Sci. 2000;3(3):469-472.

16. Genetic Resources Information System for Wheat and Triticale. Available online at http://www.wheatpedigree.net/

17. Sibikeev S.N., Voronina S.A., Badaeva E.D., Druzhin A.E. Study of resistance to leaf and stem rust in Triticum aestivum – Aegilops speltoides lines. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2015;19(2):165-170. (in Russian)

18. Gill B.S., Sharma H.C., Raupp W.J., Browder L.E., Hatchett J.H., Harvey T.L., Moseman J.G., Waines J.G. Evaluation of Aegilops species for resistance to wheat powdery mildew, wheat leaf rust, Hessian fly, and greenbug. Plant Dis. 1985;69(4):314-316.

19. Sochalova L.P. Sources of wheat resistance genes to leaf and stem pathogens in the Novosibirsk region. Zernovoe Khozjaistvo Rossii = Grain Economy of Russia. 2016;2:45-49. (in Russian)

20. Gultyaeva E.I., Aristova M.K., Shaidayuk E.L., Mironenko N.V., Kazartsev I.A., Akhmetova A., Kosman E. Genetic differentiation of Puccinia triticina Erikss. in Russia. Russ. J. Genet. 2017;53(9):9981005. DOI 10.1134/S1022795417070031.

21. Sochalova L.P., Piskarev V.V. Resistance of spring common wheat varieties to agents of infectious diseases in the changing climate of West Siberia. Dostizheniya Nauki i Tekhniki APK = Achievements of Sci-ence and Techniques of Agroindustrial Complex. 2017;31(2):21-25. (in Russian)

22. Gultyaeva E.I., Orina A.S., Gannibal Ph.B., Mitrofanova O.P., Odintsova I.G., Laikova L.I. The effectiveness of molecular markers for the identification of Lr28, Lr35, and Lr47 genes in common wheat. Russ. J. Genet. 2014;50(2):131-139. DOI 10.1134/S1022795414020069.

23. Tyryshkin L.G., Syukov V.V., Zakharov V.G., Zuev E.V., Gashimov M.E., Kolesova M.A., Chikida N.N., Yershova M.A., Belousova M.H. Sources of effective resistance of common wheat and its relatives to fungal diseases: search, creation, and use in breeding. Trudy po Prikladnoy Botanike, Genetike i Selektzii = Proceedings on Applied Botany, Genetics, and Breeding. 2012; 170:187-201. (in Russian)

24. Holubec V., Hanzalova A., Dumalasova V., Bartos P. Aegilops conser¬vation and collection evaluation in the Czech Republic. J. Syst. Evol. 2014;52(6):783-789. DOI 10.1111/jse.12117.

25. Tyunin V.А., Shreyder Е.R., Gultyaeva E.I., Shaydayuk E.L. Characteristics of virulence of Puccinia triticina populations and the potential of the Lr24, Lr25, LrSp genes for spring common wheat breeding in the Southern Ural. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2017;21(5):523529. DOI 10.18699/VJ17.269. (in Russian)

26. Koval S.F., Koval V.S., Shamanin V.P. Isogenic Lines of Wheat. Omsk: Omskblankizdat Publ., 2001. (in Russian)

27. Volkova G.V., Kremneva O.Yu., Shumilov Yu.V., Gladkova E.V., Vaganova O.F., Mitrofanova O.P., Lysenko N.S., Chikida N.N., Khakimova A.G., Zuev E.V. Immunologic estimation of accessions of wheat, its rare species, and Aegilops from the collection of N.I. Vavilov All-Russian Institute of Plant Genetic Resources and selection of sources with group resistance. Vestnik Zaschity Rastenii = Bulletin of Plant Protection. 2016;89(3):38-39. (in Russian)

28. Lapochkina I.F. Alien genetic variability and its importance in plant breeding. The identified plant gene pool and breeding. St. Petersburg: Vavilov Institute of General Genetics Publ., 2005;684-739. (in Russian)

29. Voronkova A.A., Dubonosov T.S., Panarin I.V. Causes of leaf rust outbreaks in the Krasnodar region. Rust of Cereals. Moscow: Kolos Publ., 1975;80-88. (in Russian)

30. Lapochkina I.F., Solomatin D.A., Grishina E.E., Vishnyakova Kh.S., Pukhalskiy V.A. Common wheat lines with genetic material from Aegilops speltoides Tausch. Russ. J. Genetics. 1996; 32(12):14381442.

31. Mains E.B., Jackson H.S. Physiological specialization in the leaf rust wheat Puccinia triticina Erikss. Phytopathology. 1926;16(1): 89-95.

32. Marais G.F., Bekker T.A., Eksteen B., McCallum T., Marais A.S. Attempts to remove gametocidal genes co-transferred to common wheat with rust resistance from Aegilops speltoides. Euphytica. 2010;171:71-85. DOI 10.1007/s10681-009-9996-2.

33. Markelova T.S., Ivanova O.V., Baukenova E.A., Naryshkina E.A., Salmova M.F. Creation of donors of multiple resistance to fungal diseases of spring common wheat by gene introgression from wild species and wheat relatives. Agrarnyy Vestnik Yugo-Vostoka = Agricultural Bulletin of South-East. 2014;1-2:25-27. (in Russian)

34. Martynov S.P., Dobrotvorskaya T.V., Mitrofanova O.P. Genealogical analysis of the use of aegilops (Aegilops L.) genetic material in wheat (Triticum aestivum L.) Russ. J. Genet. 2015;51(9):855-862. DOI 10.1134/s1022795415090070.

35. McIntosh R.A., Yamazaki Y., Dubcovsky J., Rogers J., Morris C., Appels R., Xia X.C. Catalogue of gene symbols for wheat. 12th Int. Wheat Genet. Symp. 8-13 September 2013, Yokohama, Japan. Available online at http://www.shigen.nig.ac.jp/wheat/

36. Meshkova L.V., Rosseeva L.P., Shreider E.R., Sidorov A.V. Virulence of pathotypes of the wheat leaf rust agent to ThLr9 in regions of Siberia and the Urals. 2nd All-Russian Conf. “Modern Problems of Plant Immunity to Harmful Organisms”. St. Petersburg, 29 September–2 October 2008. St. Petersburg, 2008;70-73. (in Russian)

37. Mikhailova L.A. Genetics of the Relationships between the Leaf Rust Agent and Wheat. St. Petersburg: VIR Publ., 2006. (in Russian)

38. Mikov D.S., Davoyan E.R., Zubanova Yu.S., Davoyan R.O., Boldakov D.M. Molecular marker-assisted identification of leaf rust resistance genes Lr28, Lr35, Lr36, Lr47, Lr51 in accessions of Aegilops speltoides, the synthetic form Avrodes, and its derivatives. Proceedings of the 9th All-Russian Conference of Young Scientists. 2016;8889. (in Russian)

39. Odintsova I.G., Agafonova N.A., Boguslavsky R.L. Introgressive lines of common wheat with resistantance to leaf rust transferred from Aegilops speltoides. Source material and problems of wheat and triticale breeding. Trudy po Prikladnoy Botanike, Genetike i Selektsii = Proceedings on Applied Botany, Genetics, and Breeding. 1991;142:106-110. (in Russian)

40. Parlevliet J.E. Durability of resistance against fungal, bacterial and viral pathogens; present situation. Euphytica. 2002;124:147-156.

41. Peterson R.F., Campbell A.B., Hannah A.E. A diagrammatic scale for estimating rust intensity of leaves and stem of cereals. Can. J. Res. 1948;Sect. C. 26:496-500.

42. Plotnikova L.Ya., Kuzmina S.P. Genetic and physiological bases of resistance to leaf rust in introgressive lines of Novosibirskaya 67 variety with genes from Aegilops speltoides Tausch. Proceedings of the 2nd All-Russian (national) scientific conference “The Role of Agrarian Science in the Sustainable Development of Rural Areas”, Novosibirsk, December 25, 2017. Novosibirsk, 2017;100-102. (in Russian)

43. Plotnikova L.Ya., Pozherukova V.E., Meshkova L.V., Mitrofanova O.P., Degtyarev A.I., Aidosova A.T. The resistance of Timofeevi wheat to Puccinia triticina in West Siberia. Mikologiya i Fitopatologiya = Mycology and Phytopathology. 2015;49(2):116-125. (in Russian)

44. Plotnikova L.Ya., Stubey T.Yu. Appearance of resistance to Puccinia triticina determined by the genes Lr13, Lr22b, and Lr35 in adult plants. Mikologiya i Fitopatologiya = Mycology and Phytopathology. 2009;43(3):258-271. (in Russian)

45. Salina E.A., Adonina I.G., Badaeva E.D., Stasyuk A.I., Leonova I.N., Shishkina A.A., Divashuk M.G., Stankova E.V., Khuat T.M., Syukov V.V., Karlov G.I. A Thinopyrum intermedium chromosome in bread wheat cultivars as a source of genes conferring resistance to fungal diseases. Euphytica. 2015;204:91-101. DOI 10.1007/s10681014-1344-5.

46. Sanin S.S. Phytosanitary monitoring: current state and ways of improvement. Problems of Optimization of the Phytosanitary Condition of Plant Growing. Proceedings of the All-Russian Congress on Plant Protection, St. Petersburg, December 1995. St. Petersburg, 1997;166-176. (in Russian)

47. Shah T.M., Ahmed J., Asghar M., Iqbal N., Farooq S. Evaluation of annual wild grass species for leaf rust resistance. Pak. J. Biol. Sci. 2000;3(3):469-472.

48. Sibikeev S.N., Voronina S.A., Badaeva E.D., Druzhin A.E. Study of resistance to leaf and stem rust in Triticum aestivum – Aegilops speltoides lines. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2015;19(2):165-170. (in Russian)

49. Sochalova L.P. Sources of wheat resistance genes to leaf and stem pathogens in the Novosibirsk region. Zernovoe Khozjaistvo Rossii = Grain Economy of Russia. 2016;2:45-49. (in Russian)

50. Sochalova L.P., Piskarev V.V. Resistance of spring common wheat varieties to agents of infectious diseases in the changing climate of West Siberia. Dostizheniya Nauki i Tekhniki APK = Achievements of Sci-ence and Techniques of Agroindustrial Complex. 2017;31(2):21-25. (in Russian)

51. Tyryshkin L.G., Syukov V.V., Zakharov V.G., Zuev E.V., Gashimov M.E., Kolesova M.A., Chikida N.N., Yershova M.A., Belousova M.H. Sources of effective resistance of common wheat and its relatives to fungal diseases: search, creation, and use in breeding. Trudy po Prikladnoy Botanike, Genetike i Selektzii = Proceedings on Applied Botany, Genetics, and Breeding. 2012; 170:187-201. (in Russian)

52. Tyunin V.А., Shreyder Е.R., Gultyaeva E.I., Shaydayuk E.L. Characteristics of virulence of Puccinia triticina populations and the potential of the Lr24, Lr25, LrSp genes for spring common wheat breeding in the Southern Ural. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2017;21(5):523529. DOI 10.18699/VJ17.269. (in Russian)

53. Volkova G.V., Kremneva O.Yu., Shumilov Yu.V., Gladkova E.V., Vaganova O.F., Mitrofanova O.P., Lysenko N.S., Chikida N.N., Khakimova A.G., Zuev E.V. Immunologic estimation of accessions of wheat, its rare species, and Aegilops from the collection of N.I. Vavilov All-Russian Institute of Plant Genetic Resources and selection of sources with group resistance. Vestnik Zaschity Rastenii = Bulletin of Plant Protection. 2016;89(3):38-39. (in Russian)

54. Voronkova A.A., Dubonosov T.S., Panarin I.V. Causes of leaf rust outbreaks in the Krasnodar region. Rust of Cereals. Moscow: Kolos Publ., 1975;80-88. (in Russian)