ОЦЕНКА ПО ТЕХНОЛОГИИ rSNP_Guide SNPs ПРОМОТОРОВ ГЕНОВ АРС И МLH1 ЧЕЛОВЕКА, СВЯЗАННЫХ С РАКОМ ТОЛСТОГО КИШЕЧНИКА

Каждый из 6 регуляторных SNPs (Single nucleotide polymorphisms) генов APC и MLH1 человека (rs75996864, rs76241113, rs78037487, rs80112297, rs80313086 и rs1800734) был оценен по созданной ранее технологии rSNP_Guide на значимость изменения связывания каждого из 40 факторов транскрипции с соответствующими районами ДНК. В результате для каждого SNP все анализируемые белки были ранжированы по убыванию уровня статистической значимости α (t-тест Стьюдента) изменения их сродства к аллельным вариантам указанной ДНК. Установлено, что самыми вероятными проявлениями SNPs rs75996864, rs76241113, rs78037487, rs80112297 и rs80313086 гена APC, а также SNP rs1800734 гена MLH1 человека являются изменения в связывании именно тех транскрипционных факторов (NF-Y, NFkB, c-Myb, RAR, YY-1, Sp-1), для которых ранее было показано участие в развитии рака толстого кишечника. Полученные результаты служат новым основанием для исследований ассоциации SNPs rs75996864, rs76241113, rs78037487, rs80112297, rs80313086 гена APC с раком толстого кишечника общепринятыми медико-генетическими методами.

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