The development and study of common wheat introgression lines derived from the synthetic form RS7

Synthetic recombination form RS7 (BBAAUS), in which the first two genomes, A and B, originate from common wheat, and the third recombinant genome consists of Aegilops speltoides (S) and Ae. umbellulata (U) chromosomes, was obtained from crossing synthetic forms Avrodes (BBAASS) and Avrolata (BBAAUU). Resistant to leaf rust, yellow rust and powdery mildew, introgression lines have been obtained from backcrosses with the susceptible varieties of common wheat Krasnodarskaya 99, Fisht and Rostislav. PCR analysis showed the presence of amplification fragments with marker SCS421 specific for the Lr28 gene in the line 4991n17. The cytological study (С-banding and FISH) of 14 lines has revealed chromosomal modifications in 12 of them. In most cases, the lines carry translocations from Ae. speltoides, which were identified in chromosomes 1D, 2D, 3D, 2B, 4B, 5B and 7B. Also, lines with the substituted chromosomes 1S (1B), 4D (4S), 5D (5S) and 7D (7S) were identified. Lines that have genetic material from Ae. speltoides and Ae umbellulata at once were revealed. In the line 3379n14, translocations in the short arm of chromosome 7D from Ae. umbellulata and chromosomes 5BL, 1DL, 2DL from Ae. speltoides were revealed. The line 4626p16 presumably has a translocation on the long arm of chromosome 2D from Ae. umbellulata and the T7SS.7SL-7DL translocation from Ae. speltoides. The T1DS.1DL-1SL and T3DS.3DL-3SL translocations from Ae. speltoides, and T2DS.2DL-2UL and T7DL.7DS-7US from Ae. umbellulata have been obtained for the first time. These lines may carry previously unidentified disease resistance genes and, in particular, leaf rust resistance genes from Ae. speltoides and Ae. umbellulata.

1. Адонина И.Г., Леонова И.Н., Бадаева Е.Д., Салина Е.А. Генотипирование сортов мягкой пшеницы разных регионов России. Вавиловский журнал генетики селекции. 2016:20(1):44-50. DOI 10.18699/VJ16.107. [Adonina I.G., Leonova I.N., Badaeva E.D., Salina E.A. Genotyping of hexaploid wheat varieties from different Russian regions. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2016;20(1):44-50. DOI 10.18699/VJ16.107. (in Russian)]

2. Адонина И.Г., Петраш Н.В., Тимонова Е.М., Христов Ю.А., Салина Е.А. Создание и изучение устойчивых к листовой ржавчине линий мягкой пшеницы с транслокациями от Aegilops speltoides Tauch. Генетика. 2012;48(4):488-494. [Adonina I.G., Petrash N.V., Timonova E.M., Khristov Y.A., Salina E.A. Construction and study of leaf rust resistant common wheat lines with translocations of Aegilops speltoides Tausch. Russ. J. Genet. 2012;48(4):404-409.]

3. Давоян Р.О., Бебякина И.В., Давоян Э.Р., Миков Д.С., Бадаева Е.Д., Адонина И.Г., Салина Е.А., Зинченко А.С., Зубанова Ю.С. Использование синтетической формы Авродес для передачи устойчивости к листовой ржавчине от Aegilops speltoides мягкой пшенице. Вавиловский журнал генетики селекции. 2017;21(6): 663-670. DOI 10.18699/VJ17.284. [Davoyan R.O., Bebyakina I.V., Davoyan E.R., Mikov D.S., Badaeva E.D., Adonina I.G., Salina E.A., Zinchenko A.N., Zubanova Y.S. Use of synthetic form Avrodes for transfer of leaf rust resistance from Aegilops speltoides to common wheat. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2017;21(6):663-670. DOI 10.18699/VJ17.284. (in Russian)]

4. Давоян Э.Р., Давоян Р.О., Бебякина И.В., Давоян О.Р., Зубанова Ю.С., Зинченко А.Н., Кравченко А.М. Идентификация генов устойчивости к листовой ржавчине в видах Aegilops L., синтетических формах и интрогрессивных линиях мягкой пшеницы. Вавиловский журнал генетики и селекции. 2012;16(1):116-122. [Davoyan E.R., Davoyan R.O., Bebyakina I.V., Davoyan O.R., Zubanova Yu.S., Kravchenko A.M., Zinchenko A.N. Identification of a leaf rust-resistance gene in species of Aegilops L., synthetic forms, and introgression lines of common wheat. Russ. J. Genet.: Appl. Res. 2012;2(4):325-329.]

5. Жиров Е.Г., Терновская Т.К. Геномная инженерия у пшеницы. Вестник с.-х. науки. 1984;10:58-66. [Zhirov E.G., Ternovskaya T.K. Genome engineering in wheat. Vestnik Selskokhozyaystvennoy Nauki = Herald of Agricultural Science. 1984;10:58-66. (in Russian)]

6. Паушева З.П. Практикум по цитологии растений. М.: Колос, 1974;75. [Pausheva Z.P. Laboratory Manual on Plant Cytology. Moscow: Kolos Publ., 1974;75. (in Russian)]

7. Пересыпкин В.Ф. Болезни зерновых культур. М.: Колос, 1979; 251-260. [Peresipkin V.F. Diseases of Grain Cultures. Moscow: Kolos Publ., 1979;251-260. (in Russian)]

8. Badaeva E.D., Badaev N.S., Gill B.S., Filatenko A.A. Interspecific karyotype divergence in Triticum araraticum. Plant Syst. Evol. 1994;192(1):117-145.

9. Bedbrook J.R., Jones J., O’Dell M., Thompson R.D., Flavell R.B. A molecular description of telomeric heterochromatin in Secale species. Cell. 1980;19:545-560. DOI 10.1016/0092-8674(80)90529-2.

10. Cherukuri D.P., Gupta S.K., Charpe A., Koul S., Prabhu K.V., Singh R.B., Haq Q.M.R. Molecular mapping of Aegilops speltoides derived leaf rust resistance gene Lr28 in wheat. Euphytica. 2005; 143:19-26.

11. Dubcovsky J., Helguera M., Khan I.A. Development of PCR markers for wheat leaf rust resistance gene Lr47. Theor. Appl. Genet. 2000; 101:625-631.

12. Friebe B., Jiang J., Raupp W.J., McIntosh R.A., Gill B.S. Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica. 1996;91:59-87.

13. Gasner G., Straib U.W. Weitere Untersuchungen uber die Spezialisierung sverhaltnissedes Gelbrostes Puccinia glumarum (Schm.) Erikss. u. Henn. Arb. Boil. Reichsanstalt. 1934;21:121-145.

14. Helguera M., Vanzetti L., Soria M., Khan I.A., Kolmer J., Dubcovsky J. PCR markers for Triticum speltoides leaf rust resistance gene Lr51 and their use to develop isogenic hard red spring wheat lines. Crop Sci. 2005;45(2):728-734.

15. Knott D.R. Transferring Alien Genes to Wheat. In: Heyne E.G. (Ed.). Wheat and Wheat Improvement. American Society of Agronomy. Madison, WI, USA, 1987;462-471.

16. Mains E.B., Jakson H.S. Physiologic specialization in leaf rust of wheat, Puccinia triticiana Erikss. Phytopatology. 1926;16:89-120.

17. Marais G.F., Bekker T.A., Eksteen A., Mccallum B., Fetch T., Marais A.S. Attempts to remove gametocidal genes co-transferred to common wheat with rust resistance from Aegilops speltoides. Euphytica. 2010;171(1):71-85.

18. McIntosh R.A., Yamazaki Y., Dubovsky J., Rogers J., Morris C., Appels R., Xia X.C. Catalogue of gene symbols for wheat. 2013. Available at: http://www.shigen.nig.ac.jp/wheat/komugi/genes.

19. Plaschke J., Ganal M.W., Röder M.S. Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor. Appl. Genet. 1995;91:1001-1007.

20. Rayburn A.L., Gill B.S. Isolation of a D-genome specific repeated DNA sequence from Aegilops squarrosa. Plant Mol. Biol. Rep. 1986;4: 102-109. DOI 10.1007/BF02732107.

21. Salina E., Adonina I., Vatolina T., Kurata N. A comparative analysis of the composition and organization of two subtelomeric repeat families in Aegilops speltoides Tausch. and related species. Genetics. 2004;122:227-237.

22. Salina E.A., Lim Y.K., Badaeva E.D., Shcherban A.B., Adonina I.G., Amosova A.V., Samatadze T.E., Vatolina T.Yu., Zoshchuk S.A., Leitch A.A. Phylogenetic reconstruction of Aegilops section Sitopsis and the evolution of tandem repeats in the diploids and derived wheat polyploids. Genome. 2006;49:1023-1035. DOI 10.1139/G06-050.

23. Schachermayer G., Siedler H., Gale M.D. Identification and localization of molecular markers linked to the Lr9 leaf rust resistance gene of wheat. Theor. Appl. Genet. 1994; 88:110-115.

24. Schneider A., Linc G., Molnar-Lang M. Fluorescence in situ hybridization polymorphism using two repetitive DNA clones in different cultivars of wheat. Plant Breed. 2003;122:396-400. DOI 10.1046/j.1439-0523.2003.00891.

25. Sears E.R. The transfer of leaf rust resistance from Aegilops umbellulata to wheat. Brookhaven Symp. Biol. 1956;9:1-22.

26. Seyfarth R., Feuillet C., Schachermayr G., Winzeler M., Keller B. Development of molecular mapping of the adult-plant leaf rust resistance gene Lr35 in wheat. Theor. Appl. Genet. 1999;99:554-560.

27. Tsatsenco L.V., Zhirov E.G., Davoyan R.O. Hybrids between wheat and genome-substituted form Avrodes. Cytogenetics and agronomy investigations. Cereal Res. Commun. 1993;21(1):45-50.