Identification of genome compositions in allopolyploid species of the genus Elymus(Poaceae: Triticeae) in the Asian part of Russia by CAPS analysis
https://doi.org/10.18699/VJ20.606
Abstract
The genus ElymusL., together with wheat, rye, and barley, belongs to the tribe Triticeae. Apart from its economic value, this tribe is characterized by abundance of polyploid taxa formed in the course of remote hybridization. Single-copy nuclear genes are convenient markers for identification of source genomes incorporated into polyploids. In the present work, a CAPS-marker is developed to distinguish basic St, H, and Y genomes comprising polyploid genomes of Asiatic species of the genus Elymus. The test is based on electrophoretic analysis of restriction patterns of a PCR-amplified fragment of the gene coding for beta-amylase. There are about 50 Elymusspecies in Russia, and most of them are supposed to possess one of three haplome combinations, StH, StY and StHY. Boreal StH-genomic species endemic for Russia are the least studied. On the basis of nucleotide sequences from public databases, TaqI restrictase was selected, as it produced patterns of restriction fragments specific for St, H, and Y haplomes easily recognizable in agarose gel. A sample of 68 accessions belonging to 32 species was analyzed. In 15 species, the earlier known genomic constitutions were confirmed, but in E. kamojithis assay failed to reveal the presence of H genome. This unusual H genome was suggested to originate from a different Hordeum species. In 16 species, genomic constitutions were identified for the first time. Fifteen accessions from Asian Russia possessed the genomic constitution StStHH, and E. amurensis, phylogenetically close to the StY-genomic species E. ciliaris, had the genomic constitution StStYY. It is inferred that the center of species diversity of the StH-genomic group is shifted to the north as compared to the center of origin of StY-genomic species, confined to China.
Supporting agencies: This work was supported by the state project “Estimation of the morphogenetic potential of the North Asian plant population by experimental methods” (state registration number: AAAA-A17-117012610051-5) for the Central Siberian Botanical Garden (CSBG) SB RAS and state project 0324-2019-0039-C-01 for the Institute of Cytology and Genetics SB RAS, the Russian Foundation for Basic Research (project No. 18-04-01030). Materials of the bioresource scientific collection of the CSBG SB RAS “Collections of living plants in open and closed ground”, USU No. 440534 were used.
About the Authors
A. V. Agafonov
Central Siberian Botanical Garden of Siberian Branch of the Russian Academy of Sciences
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
E. V. Shabanova (Kobozeva)
Central Siberian Botanical Garden of Siberian Branch of the Russian Academy of Sciences
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
S. V. Asbaganov
Central Siberian Botanical Garden of Siberian Branch of the Russian Academy of Sciences
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
A. V. Mglinets
Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
V. S. Bogdanova
Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
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