Identification of genome compositions in allopolyploid species of the genus Elymus(Poaceae: Triticeae) in the Asian part of Russia by CAPS analysis
https://doi.org/10.18699/VJ20.606
Abstract
The genus ElymusL., together with wheat, rye, and barley, belongs to the tribe Triticeae. Apart from its economic value, this tribe is characterized by abundance of polyploid taxa formed in the course of remote hybridization. Single-copy nuclear genes are convenient markers for identification of source genomes incorporated into polyploids. In the present work, a CAPS-marker is developed to distinguish basic St, H, and Y genomes comprising polyploid genomes of Asiatic species of the genus Elymus. The test is based on electrophoretic analysis of restriction patterns of a PCR-amplified fragment of the gene coding for beta-amylase. There are about 50 Elymusspecies in Russia, and most of them are supposed to possess one of three haplome combinations, StH, StY and StHY. Boreal StH-genomic species endemic for Russia are the least studied. On the basis of nucleotide sequences from public databases, TaqI restrictase was selected, as it produced patterns of restriction fragments specific for St, H, and Y haplomes easily recognizable in agarose gel. A sample of 68 accessions belonging to 32 species was analyzed. In 15 species, the earlier known genomic constitutions were confirmed, but in E. kamojithis assay failed to reveal the presence of H genome. This unusual H genome was suggested to originate from a different Hordeum species. In 16 species, genomic constitutions were identified for the first time. Fifteen accessions from Asian Russia possessed the genomic constitution StStHH, and E. amurensis, phylogenetically close to the StY-genomic species E. ciliaris, had the genomic constitution StStYY. It is inferred that the center of species diversity of the StH-genomic group is shifted to the north as compared to the center of origin of StY-genomic species, confined to China.
Supporting agencies: This work was supported by the state project “Estimation of the morphogenetic potential of the North Asian plant population by experimental methods” (state registration number: AAAA-A17-117012610051-5) for the Central Siberian Botanical Garden (CSBG) SB RAS and state project 0324-2019-0039-C-01 for the Institute of Cytology and Genetics SB RAS, the Russian Foundation for Basic Research (project No. 18-04-01030). Materials of the bioresource scientific collection of the CSBG SB RAS “Collections of living plants in open and closed ground”, USU No. 440534 were used.
About the Authors
A. V. Agafonov
Central Siberian Botanical Garden of Siberian Branch of the Russian Academy of Sciences
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
E. V. Shabanova (Kobozeva)
Central Siberian Botanical Garden of Siberian Branch of the Russian Academy of Sciences
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
S. V. Asbaganov
Central Siberian Botanical Garden of Siberian Branch of the Russian Academy of Sciences
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
A. V. Mglinets
Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
V. S. Bogdanova
Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
References
1. Agafonov A.V., Asbaganov S.V., Shabanova (Kobozeva) E.V., Morozov I.V., Bondar A.A. Genome constitution and differentiation of subgenomes in Siberian and Far Eastern endemic species of the genus Elymus (Poaceae) according to the sequencing of the nuclear gene waxy. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2019;23(7):817-826. DOI 10.18699/VJ19.555. (in Russian)
2. Agafonov A.V., Kobozeva E.V., Asbaganov S.V., ShmakovN.A. Current achievements and prospects of construction of a phylogenetically oriented taxonomy of the genus Elymus(Poaceae: Triticeae). Proceedings of the 14th International Scientific and Practical Conference “Problems of Botany of Southern Siberia and Mongolia”. Barnaul. 2015;314-322. (in Russian)
3. Barkworth M.E. Changing perceptions of the Triticeae. In: Jacobs S.W.L., Everett J. (Eds.). Grasses: Systematics and Evolution. Melbourne: CSIRO, 2000;110-120.
4. Barkworth M.E., Cambell J.J.N., Salomon B. Elymus L. In: Barkworth M.E., Capels K.M., Long S., Anderton L.K., Piep M.B. (Eds.). Flora of North America. New York; Oxford: Oxford University Press, 2007;24:288-343.
5. Baum B.R., Yang J.-L., Yen C., Agafonov A.V. A taxonomic revision of the genus CampeiostachysDrobov. J. Syst. Evol. 2011;49(2):146- 159.
6. Dewey D.R. Synthetic Agropyron-Elymus hybrids. III. Elymus canadensis × Agropyron caninum, A. trachycaulumand A. striatum. Amer. J. Bot. 1968;55:1133-1139.
7. Dewey D.R. Cytogenetics of Elymus sibiricusand its hybrids with Agropyron tauri, Elymus canadensisand Agropyron caninum.Bot. Gaz. 1974;135:80-87.
8. Dewey D.R. Cytogenetics of Agropyron ugamicumand six of its interspecific hybrids. Bot. Gaz. 1980;141:305-312.
9. Dewey D.R. The genomic system of classification as a guide to intergeneric hybridization with the perennial Triticeae. In: Gustafson J.P. (Ed.). Gene Manipulation in Plant Improvement. New York: Plenum Publ. Corp., 1984;209-279.
10. Gostimsky S.A., Kokaeva Z.G., Konovalov F.A. Studying plant genome variation using molecular markers. Russ. J. Genet. 2005;41(4): 378-388. DOI 10.1007/s11177-005-0101-1.
11. Hu C.Y., Tsai Y.Z., Lin S.F. Development of STS and CAPS markers for variety identification and genetic diversity analysis of tea germplasm in Taiwan. Bot. Stud. 2014;55:12. DOI 10.1186/1999-3110-55-12.
12. Jensen K.B. Cytology, fertility, and origin of Elymus abolinii(Drob.) Tzvelev and its F1 hybrids with Pseudoroegneria spicata, E. lan ceolatus, E. dentatusssp. ugamicus, and E. drobovii(Poaceae: Triti ceae). Genome. 1989;32:468-474. DOI 10.1139/g89-470.
13. Jensen K.B. Cytology and morphology of Elymus pendulinus, E. pendulinusssp. multiculmis, and E. parviglume (Poaceae: Triticeae). Bot. Gaz. 1990;151(2):245-251.
14. Jensen K.B., Hatch S. Genome analysis, morphology, and taxonomy of Elymus gmeliniiand E. strictus(Poaceae: Triticeae). Bot. Gaz. 1989;150(1):84-92.
15. Jensen K.B., Wang R.R.C. Cytogenetics of Elymus caucasicusand Elymus longearistatus(Poaceae: Triticeae). Genome. 1991;34:860- 867.
16. Kobozeva E.V., Asbaganov S.V., Agafonov A.V. Genome composition and assessment of the divergence between Russian boreal species in the genus Elymus (Poaceae) detected on the basis of sequen cing of the nuclear gene GBSSI. In: Prospects of Development and Challenges of Modern Botany. BIO Web Conf. 2018;11.00023. DOI 10.1051/bioconf/20181100023.
17. Kobozeva E.V., MglinetsA.V., Agafonov A.V. Identification of the genomic composition in allopolyploid species of the genus Elymus (Poaceae: Triticeae) with CAPS-analysis. In: Proceedings of the 6th International Conference “Issues in the Study of the Vegetation Cover in Siberia”. Tomsk. 2017;155-157. DOI 10.17223/9785946216371/51. (in Russian)
18. Konieczny A., Ausubel F.M. A procedure for mapping Arabidopsis mutations using codominant ecotypespecific PCR-based markers. Plant J. 1993;4(2):403-410.
19. Li X.-M., Lee B.S., Mammadov A.C., Koo B.C., Mott I.W., Wang R.R.C. CAPS markers specific to Eb, Ee, and R genomes in the tribe Triticeae. Genome. 2007;50:400-411.
20. Liu C.W., Dewey D.R. The genome constitution of Elymus fedtschenkoi.Acta Genet. Sinica. 1983;10:20-27.
21. Löve A. Conspectus of the Triticeae. Feddes Repert. 1984;95:425-521.
22. Lu B.-R., Bothmer R. von. Interspecific hybridization with Elymus himalayanus and E. schrenkianus, and other Elymusspecies (Triticeae: Poaceae). Genome. 1992;35:230-237.
23. Lu B.-R., Salomon B. Differentiation of the SY genomes in Asiatic Elymus.Hereditas. 1992;116:121-126.
24. Lu B.-R., Salomon B., Bothmer R. von. Interspecific hybridization with Elymus confususand E. dolichaterus, and their genomic relation ships (Poaceae: Triticeae). Plant Syst. Evol. 1995;197:1-17.
25. Mason-Gamer R.J. Origin of North American Elymus (Poaceae: Triticeae) allotetraploids based on granulebound starch synthase gene sequences. Syst. Bot. 2001;26:757-768.
26. Mason-Gamer R.J. Phylogeny of a genomically diverse group of Elymus(Poaceae) allopolyploids reveals multiple levels of reticulation. PLoS One. 2013;8(11):e78449. DOI 10.1371/journal.pone.0078449.
27. Okito P., Mott I.W., Wu Y., Wang R.R. A Y genome specific STS marker in Pseudoroegneriaand Elymusspecies (Triticeae: Gramineae). Genome. 2009;52(4):391-400.
28. Shavrukov Y.N. CAPS markers in plant biology. Russ. J. Genet.: Appl. Res. 2016;6(3):279-287. DOI 10.1134/S2079059716030114.
29. Tsvelyov N.N. On the genus Elymus(Poaceae) in Russia. Botaniches kiy Zhurnal = Botanical Journal. 2008;93(10):1587-1596. (in Rus sian)
30. Tsvelyov N.N., Probatova N.S. The genera Elymus L., Elytrigia Desv., Agropyron Gaertn., Psathyrostachys Nevski, and Leymus Hochst. (Poaceae: Triticeae) in the flora of Russia. In: V.L. Komarov Memorial Lectures. Vladivostok: Dalnauka Publ., 2010;57:5-102. (in Russian)
31. Zhou Y.-H., Wu B.-H., Fu T.-H., Zheng Y.L. Morphology, fertility and cytogenetics of intergeneric hybrid between Roegneria kamoji Ohwi and Dasypyrum villosum(L.) Candargy (Poaceae: Triticeae). J. Syst. Evol. 1999;37(2):125-130.
Review
Views:
837
Email this article 