Resistance to causal agents of late blight and golden potato nematode of the modern cultivars of seed potatoes and their phytosanitary status in various agroclimatic zones of the European part of Russia

The active expansion of foreign potato cultivars on the territory of the Russian Federation has led to a change in the dominant pathogen species and to the emergence of new pathotypes of causal agents of harmful potato diseases. The aim of the study was to evaluate resistance to Phytophthora infestans and Globodera rostochiensis of modern potato cultivars and determine the distribution of fungal and oomycetic diseases on potato cultivars in various agroclimatic zones of Russia. The resistance of 41 foreign cultivars was evaluated to pathotype Ro1 G. rostochiensis and to isolate VZR17 P. infestans with virulence genes 1.2.3.4.5.6.7.8.9.10.11. Resistant to G. rostochiensis were 38 cultivars. 57R marker of the H1 gene conferring resistance to the Ro1 pathotype of G. rostochiensis was detected in 96.6 % of the nematode resistant cultivars studied; susceptible varieties did not possess this marker. Absolute resistance to the causative agent of late blight was demonstrated by the cultivars Alouette and Sarpo Mira (score 9); high levels of resistance (score 6 and 7) were determined for the cultivars Evolution, Red Fantasy and Ricarda. The cultivars Baltic Rose, Damaris, Desiree, Gala, Labella, Laperla, Mia, Sanibel, Zekura, Queen Anne, Red Lady and ‘7 for 7’ were classified as susceptible, although the characteristics of originators indicated average resistance to late blight. A phytopathological test was conducted on 92 samples of 39 varieties of seed potatoes from four federal districts of the Russian Federation: Volga, NorthWest, Central and North Caucasus. Rhizoctonia solani, Fusarium spp. and Helminthosporium solani are most common on all varieties. 100 % defeat of tubers by H. solani was recorded in various regions on the cultivars Red Scarlett, Evolution, Labella, Colombo, Gala and Nevsky. Widespread Colletotrichum coccodes on tubers of the elite and 2nd reproductions of the potato cultivar Red Scarlett (50.0–71.4 %) was recorded in the Central District.

distribution of potato diseases, Phytophthora infestans, Globodera rostochiensis, potato cultivars, resistance to diseases, Helminthosporium solani, Colletotrichum coccodes, DNA markers

1. Alekhin V.T., Mikhaylikova V.V., Mikhina N.G. Economic Harmfulness Thresholds of Pests, Diseases, and Weeds in Crop Fields. Moscow: Rosinformagrotekh Publ., 2016. (in Russian)

2. Antonova O.Y., Shvachko N.A., Novikova L.Y., Shuvalov O.Y., Kostina L.I., Klimenko N.S., Shuvalova A.R., Gavrilenko T.A. Genetic diversity of potato varieties bred in Russia and its neighboring countries based on the polymorphism of SSR-loci and markers associated with resistance R-genes. Russ. J. Genet.: Appl. Res. 2017; 7(5):489-500. DOI 10.1134/S2079059717050021.

3. Gavrilenko Т.А., Klimenko N.S., Antonova O.Yu., Lebedeva V.A., Evdokimova Z.Z., Gadjiyev N.M., Apalikova O.V., Alpatyeva N.V., Kostina L.I., Zoteyeva N.M., Mamadbokirova F.T., Egorova K.V. Molecular screening of potato varieties bred in the northwestern zone of the Russian Federаtion. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2018;22(1):35-45. DOI 10.18699/VJ18.329. (in Russian)

4. State Standard 33996-2016. Seed Potatoes. Specifications and methods of determining the quality. 2017. (in Russian)

5. The State Register of Selection Achievements Authorized for Use for Production Purposes. Vol. 1. Plant Varieties (official publication). Moscow: Rosinformagrotekh Publ., 2019. (in Russian)

6. Elansky S.N. Features of the development of late blight in Russia. Zashhita Kartofelya = Potato Protection. 2015;1:8-11. (in Russian)

7. Ignatov A.N., Panycheva Yu.S., Voronina M.V., Vasiliev D.M., Dzhalilov F.S.-U. Dynamics of species composition of potato pathogens in the European part of the Russian Federation. Kartofel i Ovoshchi = Potatoes and Vegetables. 2019;9:28-32. DOI 10.25630/PAV.2019.57.62.003. (in Russian)

8. Klimenko N.S., Antonova O.Y., Kostina L.I., Mamadbokirova F.T., Gavrilenko T.A. Marker-associated selection of Russian potato varieties with using markers of resistance genes to the golden potato cyst nematode (pathotype Ro1). Trudy po Prikladnoy Botanike, Genetike i Selektsii = Proceedings on Applied Botany, Genetics, and Breeding. 2017;178(4):66-75. DOI 10.30901/2227-8834-2017-4-66-75. (in Russian)

9. Kuznetsova M.A., Kozlovsky B.E., Beketova M.P., Sokolova E.A., Malyuchenko O.P., Alekseev Y.I., Rogozina E.V., Khavkin E.E. Phytopathological and molecular characterization of Phytophthora infestans isolates collected on resistant and susceptible potato genotypes. Mikologiya i Fitopatologiya = Mycology and Phytopathology. 2016;50(3):175-184. (in Russian)

10. Simakov E.A. (Ed.). Potato Varieties Bred in Russia. Moscow, 2018. (in Russian)

11. Abbas M.F., Naz F., Irshad G. Important fungal diseases of potato and their management – a brief review. Mycopath. 2013;11(1):45-50.

12. AHDB. Agriculture and Horticulture Development Board. 2018. Available at: https://potatoes.ahdb.org.uk/agronomy/plant-health-weedpest-disease-management

13. Armstrong M.R., Vossen J., Lim T.Y., Hutten R.C.B., Xu J., Strachan S.M., Harrower B., Champouret N., Gilroy E.M., Hein I. Tracking disease resistance deployment in potato breeding by enrichment sequencing. Plant Biotechnol. J. 2019;17:540-549. DOI 10.1111/pbi.12997.

14. Bakker E., Achenbach U., Bakker J., van Vliet J., Peleman J., Segers B., van der Heijden S., van der Linde P., Graveland R., Hutten R., van Eck H., Coppoolse E., van der Vossen E., Bakker J., Goverse A. A high-resolution map of the H1 locus harbouring resistance to the potato cyst nematode Globodera rostochiensis. Theor. Appl. Genet. 2004;109(1):146-152. DOI 10.1007/s00122-004-1606-z.

15. Brylińska M., Śliwka J. Laboratory assessment of potato resistance to Phytophthora infestans. Plant Breed. Seed Sci. 2017;76:17-23. DOI 10.1515/plass-2017-00016.

16. Collins W.W. The global initiative on late blight – alliance for the future. In: Khurana S.M.P., Shekhawat G.S., Singh B.P., Pandey S.K. (Eds.). Potato Global Research and Development. Indian Potato Association. CPRI, Shimla, India, 2000;1:513-524.

17. Compendium of Potato Diseases. Hooker W.J. (Ed.). The Disease Compendia Series by the American Phythopathological Society. Publ. by the Am. Phythopathol. Soc., USA, 1981.

18. Compendium of Potato Diseases, Second Edition. Stevenson W.R., Loria R., Franc G.D., Weingartner D.P. (Eds.). St. Paul, USA: Am. Phytopathol. Soc. Press, 2001.

19. Dalamu, Bhardwaj V., Umamaheshwari R., Sharma R.P., Kaushik S.K., Joseph T.A., Singh B.P., Gebhardt C. Potato cyst nematode (PCN) resistance: genes, genotypes and markers – an update. SABRAO J. Breed. Genet. 2012;44(2):202-228.

20. Dillard H.R. Colletotrichum coccodes: the pathogen and its hosts. In: Bailey J.A., Jeger M.J. (Eds.). Colletotrichum: Biology, Pathology and Control. CAB International, Oxford, 1992;225-236.

21. Diseases, Pests and Disorders of Potatoes: A Color Handbook. Wale S.J., Platt H.W., Cattlin N.D. Elsevier, 2008.

22. Finkers-Tomczak A., Bakker E., Boer J., Vossen E., Achenbach U., Golas T., Suryaningrat S., Smant G., Bakker J., Goverse A. Comparative sequence analysis of the potato cyst nematode resistance locus H1 reveals a major lack of co-linearity between three haplotypes in potato (Solanum tuberosum ssp.). Theor. Appl. Genet. 2011;122: 595-608. DOI 10.1007/s00122-010-1472-9.

23. Gavrilenko T., Antonova O., Shuvalova A., Krylova E., Alpatyeva N., Spooner D.M., Novikova L. Genetic diversity and origin of cultivated potatoes based on plastid microsatellite polymorphism. Genet. Resour. Crop Evol. 2013;60(7):1997-2015. DOI 10.1007/s10722013-9968-1.

24. Gudmestad N.C., Taylor R.J., Pasche J.S. Management of soilborne diseases of potato. Australas. Plant Pathol. 2007;36:109-115.

25. Haldar K., Kamoun S., Hiller N.L., Bhattacharje S., van Ooij C. Common infection strategies of pathogenic eukaryotes. Nat. Rev. Microbiol. 2006;4:922-931.

26. Haverkort A.J., Struik P.C., Visser R.G.F., Jacobsen E. Applied biotechnology to combat late blight in potato caused by Phytophthora infestans. Potato Res. 2009;52:249-264. DOI 10.1007/s11540-009-9136-3.

27. Johnson D.A. Effect of foliar infection caused by Colletotrichum coccodes on yield of Russet Burbank potato. Plant Dis. 1994;78(11): 1075-1078.

28. Johnson D.A., Miliczky E.R. Effects of wounding and wetting duration on infection of potato foliage by Colletotrichum coccodes. Plant Dis. 1993;77:13-17.

29. Judelson H.S., Blanco F.A. The spores of Phytophthora: weapons of the plant destroyer. Nat. Rev. Microbiol. 2005;3:47-57.

30. Lees A.K., Hilton A.J. Black dot (Colletotrichum coccodes): an increasingly important disease of potato. Plant Pathol. 2003;52:3-12.

31. Limantseva L., Mironenko N., Shuvalov O., Antonova O., Khiutti A., Novikova L., Afanasenko O., Spooner D., Gavrilenko T. Characterization of resistance to Globodera rostochiensis pathotype Ro1 in cultivated and wild potato species accessions from the Vavilov Institute of Plant Industry. Plant Breed. 2014;133(5):660-665.

32. OEPP/EPPO. Testing of potato varieties to assess resistance to Globodera rostochiensis and Globodera pallida. OEPP/EPPO Bull. 2006; 36:419-420.

33. Oerke E.-C. Crop losses to pests. J. Agric. Sci. 2006;144:31-43. Available at: https://www.cambridge.org/core/journals/journal-ofagricultural-science/article/crop-losses-to-pests/AD61661AD6D503577B3E73F2787FE7B2

34. Rietman H., Bijsterbosch G., Cano L.M., Lee H.-R., Vossen J.H., Jacobsen E., Visser R.G.F., Kamoun S., Vleeshouwers V.G.A.A. Qualitative and quantitative late blight resistance in the potato cultivar Sarpo Mira is determined by the perception of five distinct RXLR effectors. MPMI. 2012;25(970):910-919. DOI 10.1094/MPMI-01-12-0010-R.

35. Schultz L., Cogan N.О.I., McLean K., Dale M.F.B., Bryan G.J., Forster J.N.W., Slater A.T. Evaluation and implementation of a potential diagnostic molecular marker for H1-conferred potato cyst nematode resistance in potato (Solanum tuberosum L.). Plant Breed. 2012; 131:315-321. DOI 10.1111/j.1439-0523.2012.01949.x.

36. Tsror L. Biology, epidemiology and management of Rhizoctonia solani on potato. J. Phytopathol. 2010;158:649-658. DOI 10.1111/j.14390434.2010.01671.x.

37. Tsror L., Erlich O., Hazanovsky M. Effect of Colletotrichum coccodes on potato yield, tuber quality and stem colonization during spring and autumn. Plant Dis. 1999;83(6):561-565.

38. Turner S.J. Sample preparation, soil extraction and laboratory facilities for the detection of potato cyst nematodes. In: Marks R.J., Brodie B.B. (Eds.). Potato Cyst Nematodes: Biology, Distribution and Control. CAB International, Wallingford, 1998;75-90.

39. UNECE Standard S-1 concerning the marketing and commercial quality control of seed potatoes: UNECE Guide to Seed Potato Diseases, Pests and Defects. United Nations, New York; Geneva, 2014.

40. UNECE Standard S-1 concerning the marketing and commercial quality control of seed potatoes. United Nations, New York; Geneva, 2018.

41. Winslow R.D., Willis R.J. Nematode diseases of potatoes. II. Potato cyst nematode, Heterodera rostochiensis. In: Webster J. (Ed.). Economic Nematology. N.Y.: Acad. Press, 1972;18-34.

42. Yanagisawa H., Yosuke M., Khiutti A., Mironenko N., Yasuo O., Afanasenko O. Complete genome sequence of a divergent strain of potato virus P isolated from Solanum tuberosum in Russia. Arch. Virol. 2019;164:2891-2894. DOI 10.1007/s00705-019-04397-5.