MICROSATELLITE GENOTYPING OF DON LOCAL GRAPE (VITIS VINIFERA L.) VARIETIES
Abstract
Local traditional varieties constitute an important portion of grape genetic resources. DNA profiles for microsatellite loci VVMD5, VVMD7, VVMD27, VVS2, VrZAG62, and VrZAG79 were obtained for 11 local grape cultivars grown in the Don region. These SSR-markers are recommended as basic for Vitis vinifera genotyping. Chardonnay and Cabernet Sauvignon were used as reference cultivars. The markers were combined in multiple sets for simultaneous evaluation of two loci. The total number of different alleles per locus varied from 5 (VVS2, VMD7) to 11 (VVMD5) within the Don cultivars analyzed. The cultivars possessed different sets of allele combinations for loci under study. The genotyping results were subject to cluster analysis. Sibir’kovyi and Pukhlyakovskiy belyi showed the greatest proximity to each other. Molecular studies of local southern Russia grape cultivars will be continued.
About the Authors
E. T. Ilnitskaya
North Caucasian Zonal Research Institute for Horticulture and Viticulture, Russian Academy of Agricultural Sciences, Krasnodar, Russia
Russian Federation
Russian Federation
S. V. Tokmakov
North Caucasian Zonal Research Institute for Horticulture and Viticulture, Russian Academy of Agricultural Sciences, Krasnodar, Russia
Russian Federation
Russian Federation
I. I. Suprun
North Caucasian Zonal Research Institute for Horticulture and Viticulture, Russian Academy of Agricultural Sciences, Krasnodar, Russia
Russian Federation
Russian Federation
References
1. Алиев А.М., Кравченко Л.В., Наумова Л.Г. Происхождение донских сортов винограда // Виноделие и виноградарство. 2005. № 3. С. 36–37.
2. Алиев А.М., Кравченко Л.В., Наумова Л.Г., Ганич В.А. Донские аборигенные сорта винограда. Новочеркасск: Изд-во ЮРГТУ, 2006. 84 с.
3. Девятова Н.М., Бельтюкова Н.Н., Назаров А.В. Анализ генетического разнообразия Poa pratensis L. в условиях нефтяного загрязнения почв с использованием IRАР-маркеров // Аграрн. вестн. Урала. 2011. № 1. С. 18–20.
4. Турок Й., Маградзе Д.Н., Трошин Л.П. Сохранение генофонда евразийского винограда – первостепенная проблема европейских ампелографов // Науч. журн. КубГАУ [Электронный ресурс]. Краснодар: КубГАУ, 2006. № 01 (17). Режим доступа: http://www.ej.kubagro.ru/2006/01/19/
5. Шибата Д.К. Полимеразная цепная реакция и молекулярно-генетический анализ биоптатов // Молекуляр. клинич. диагностика. М.: Мир, 1999. С. 395–427.
6. Bowers J.E., Dangl G.S., Vignani R., Meredith C.P. Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L.) // Genome. 1996. V. 39. P. 628–633.
7. Dzhambazova T., Tsvetkov I., Atanassov I. et al. Genetic diversity in native Bulgarian grapevine germplasm (Vitis vinifera L.) based on nuclear and chloroplast microsatellite polymorphisms // Vitis. 2009. V. 48. No. 3. P. 115–121.
8. Ibanez J., Andres M.T., Molino A., Borrego J. Genetic study of key Spanish grapevine varieties using microsatellites analysis // Am. J. Enol. Vitic. 2003. V. 54. P. 22–29.
9. Kimura M., Crow J.F. The number of alleles that can be maintained in a Рnite population // Genetics (US). 1964. V. 49. P. 725–738.
10. Martin J.P., Borrego J., Cabello F., Ortrız J.M. Characterization of Spanish grapevine cultivar diversity using sequencetagged microsatellite site markers // Genome. 2003. V. 46. P. 10–18.
11. Martinez L.E., Cavagnaro P.F., Masuelli R.W., Zuniga M. SSR-based assessment of genetic diversity in South American Vitis vinifera varieties // Plant Sci. 2006. V. 170. P. 1036–1044.
12. Nei M., Li W.-H. Mathematical model for studying genetic variation in terms of restriction endonucleases // Proc. Natl Acad. Sci. USA. 1979. V. 76. P. 5269–5273.
13. Rogers S.O., Bendich A.J. Extraction of DNA from milligram amounts of fresh, herbarium and mummifi ed plant tissues // Plant Mol. Biol. 1985. V. 19. No. 1. P. 69–76.
14. Schlotterer C., Soller M. Polymorphism and locus-specific effects on polymorphism at microsatellite loci in natural Drosophila melanogaster populations // Genetics. 1997. V.146. P. 309-320.
15. Stajneri N., Korosec-Korusa Z., Rusjan D., Javornic B. Microsatellite genotyping of old Slovenian grapevine varieties (Vitis vinifera L.) of the Primorje (coastal) winegrowing region // Vitis. 2008. V. 47. No. 4. P. 201–204.
16. This P., Jung A., Boccacci P. et al. Development of a standard set of microsatellite reference alleles for identifi cation of grape cultivars // Theor. Appl. Genet. 2004. V. 109. P. 1448–1458.
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