A prebreeding study of introgression spring bread wheat lines carrying combinations of stem rust resistance genes, Sr22+Sr25 and Sr35+Sr25
https://doi.org/10.18699/VJ21.081
Abstract
The Sr22, Sr35, and Sr25 genes attract the attention of bread wheat breeders with their effectiveness against Puccinia graminis f. sp. tritici race Ug99 and its biotypes. The effectiveness and impact of Sr22+Sr25 and Sr35+Sr25 gene combinations on agronomic traits have not yet been studied. In the present article, these traits were studied using the spring bread wheat lines L503/W3534//L503, L503/Sr35//L503/3/L503 carrying the Sr22+Sr25 and Sr35+Sr25 genes during 2016–2020. These lines were assessed for resistance to P. graminis f. sp. tritici under natural epiphytotics and to the Saratov, Lysogorsk and Omsk populations of the pathogen and to the PgtZ1 (TKSTF) and PgtF18.6 fungus isolates in laboratory conditions (TKSTF + Sr33). The presence of the studied Sr-genes was confirmed by using molecular markers. Prebreeding studies were conducted during 2018–2020 vegetation periods. Under the natural epiphytotics of the pathogen and in the laboratory conditions, the Sr22+Sr25 combination was highly effective, while Sr35+Sr25 was ineffective. For grain yield, the lines with the Sr22+Sr25 and Sr35+Sr25 genes were superior to the recipient cultivar L503 in one year (Sr22+Sr25 in 2019; Sr35+Sr25 in 2018), with a decrease in 2020, but in general there were no differences. For the period 2018–2020, both combinations showed a decrease in 1000 grains weight and an increase in the germination-earing period. The line with Sr22+Sr25 genes showed insignificant effects on gluten and dough tenacity, but the ratio of dough tenacity to extensibility was higher, and flour strength, porosity and bread volume were lower; in the line with Sr35+Sr25 genes, the gluten content was lower, but the strength, tenacity of the dough and the ratio of dough tenacity to extensibility were higher, flour strength and the porosity of the bread were at the recipient level, but the volume of bread was lower.
Keywords
Supporting agencies: This work was supported by the Russian Foundation for Basic Research, grant No. 18-016-00170 a.
About the Authors
S. N. Sibikeev
Federal Center of Agriculture Research of the South-East Region
Russian Federation
Russian Federation
Saratov
O. A. Baranova
All-Russian Institute of Plant Protection
Russian Federation
Russian Federation
Pushkin, St. Petersburg
A. E. Druzhin
Federal Center of Agriculture Research of the South-East Region
Russian Federation
Russian Federation
Saratov
References
1. Badaeva E.D., Ruban A.S., Shishkina A.A., Sibikeev S.N., Druzhin A.E., Surzhikov S.A., Dragovich A.Yu. Genetic classification of Aegilops columnaris Zhuk. (2n = 4x = 28, UcUcXcXc) chromosomes based on FISH analysis and substitution patterns in common wheat Ae. columnaris introgression lines. Genome. 2018;61(2):131-143.
2. Baranova O.A. Molecular identification of stem rust resistance genes in new regional wheat varieties. Vestnik Zashchity Rasteniy = Plant Protection News. 2020;103(2);113-118. DOI 10.31993/2308-6459-2020-103-2-4936. (in Russian)
3. Baranova O.A., Sibikeev S.N., Druzhin A.E. Molecular identification of the stem rust resistance genes in the introgression lines of spring bread wheat. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2019;23(3):296-303. DOI 10.18699/VJ19.494.
4. Baranova O.A., Sibikeev S.N., Druzhin A.E., Gultyaeva E.I. Resistance of spring bread wheat lines developed by ARISER to abiotic stress and rust pathogens. In: Kochetov A.V., Salina E.A. (Ed.). Plant Genetics, Genomics, Bioinformatics and Biotechnology. The 6th Intern. Scientific Conf. 14-18 June 2021. Novosibirsk. Novosibirsk, 2021. DOI 10.18699/PlantGen2021-016.
5. Gerechter-Amati Z.K., Wahl I., Vardi A., Zohary D. Transfer of stem rust seedling resistance from wild diploid einkorn to tetraploid durum wheat by means of a triploid hybrid bridge. Euphytica. 1971; 20:281-285.
6. Gultyaeva E., Sibikeev S., Druzhin A., Shaydayuk E.L. Leaf rust resistance genes identification in the spring bread wheat breeding material of the Agricultural Research Institute for South-East Regions of Russia. Ratar. Povrt. 2019;56(2):34-40. DOI 10.5937/ratpov56-20733.
7. Gultyaeva E.I., Sibikeev S.N., Druzhin A.E., Shaydayuk E.L. Enlargement of genetic diversity of spring bread wheat resistance to leaf rust (Puccinia triticina Erics.) in Lower Volga region. Selskokhozyaystvennaya Biologiya = Agricultural Biology. 2020;55(1): 27-44. DOI 10.15389/agrobiology.2020.1.27rus. (in Russian)
8. Haile J.K., Hammer K., Badebo A., Singh R.P., Röder M.S. Haplotype analysis of molecular markers linked to stem rust resistance genes in Ethiopian improved durum wheat varieties and tetraploid wheat landraces. Genet. Resour. Crop. Evol. 2013;60:853-864. DOI 10.1007/s10722-012-9880-0.
9. Jin Y., Singh R.P., Ward R.W., Wanyera R., Kinyua M., Njau P., Fetch T., Pretorius Z.A., Yahyaoui A. Characterization of seedling infection types and adult plant infection responses of monogenic Sr gene lines to race TTKS of Puccinia graminis f. sp. tritici. Plant Dis. 2007;91:1096-1099.
10. Kerber E.R., Dyck P.L. Inheritance of stem rust resistance transferred from diploid wheat (Triticum monococcum) to tetraploid and hexaploid wheat and chromosome location of gene involved. Can. J. Genet. Cytol. 1973;15:397-409.
11. Khan R., Bariana H., Dholakia B., Naik S., Lagu M., Rathjen A., Bhavani S., Gupta V. Molecular mapping of stem and leaf rust resistance in wheat. Theor. Appl. Genet. 2005;111:846-850. DOI 10.1007/s00122-005-0005-4.
12. Leonova I.N. Influence of alien genetic material on the manifestation of agronomically important traits of common wheat (T. aestivum L.). Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2018;22(3):321-328. DOI 10.18699/VJ18.367. (in Russian)
13. Luo M.C., Yang Z.L., Kota R.S., Dvorak J. Recombination of chromosomes 3A(m) and 5A(m) of Triticum monococcum with homeologous chromosomes 3A and 5A of wheat: The distribution of recombination across chromosomes. Genetics. 2000;154:1301-1308.
14. McIntosh R.A. Preemptive breeding to control wheat rusts. Euphytica. 1992;63:103-113.
15. McIntosh R.A., Brown G.N. Anticipatory breeding for resistance to rust diseases in wheat. Annu. Rev. Phytopatol. 1997;35:311-326.
16. McIntosh R.A., Dubcovsky J., Rogers W.J., Morris C., Appels R., Xia X.C. Catalogue of Gene Symbols for Wheat: 2015-2016
17. Supplement. Annual Wheat Newsletter. KSU, USA. 2016;62:102- 115.
18. McIntosh R.A., Dubcovsky J., Rogers W.J., Xia X.C., Raupp W.J. Catalogue of Gene Symbols for Wheat: 2018 Supplement. Annual Wheat Newsletter KSU, USA. 2018;64:73-93.
19. McIntosh R.A., Dubcovsky J., Rogers W.J., Xia X.C., Raupp W.J. Catalogue of Gene Symbols for Wheat: 2020 Supplement. Annual Wheat Newsletter KSU, USA. 2020;66:109-128.
20. McIntosh R.A., Wellings C.R., Park R.F. Wheat Rusts: An Atlas of Resistance Genes. Melbourne: CSIRO Publ., 1995. DOI 10.1007/978-94-011-0083-0.
21. McIntosh R.A., Yamazaki Y., Dubcovsky J., Rogers W.J., Morris C., Appels R., Xia X.C. Catalogue of gene symbols for wheat. In: 12th Int. Wheat Genet. Symp., 8-13 September 2013. Yokohama, Japan, 2013;29-30.
22. Miralles D.J., Resnicoff E., Carrtero R. Yield improvement associated with Lr19 translocation in wheat: which plant attributes are modified? In: Spiertz J.H.J., Struik P.C., van Laar H.H. (Eds.). Scale and Complexity in Plant Systems Research: Gene-Plant-Crop Relations. Dordrecht: Springer, 2007;169-176.
23. Olson E.L., Brown-Guedira G., Marshall D., Stack E., Bowden R.L., Jin Y., Rouse M., Pumphrey M.O. Development of wheat lines having a small introgressed segment carrying stem rust resistance gene Sr22. Crop Sci. 2010;50:1823-1830. DOI 10.2135/cropsci2009.11.0652.
24. Paull J.G., Pallotta M.A., Langridge P. The T.T. RFLP markers associated with Sr22 and recombination between chromosome 7A of bread wheat and the diploid species Triticum boeoticum. Theor. Appl. Genet. 1994;89:1039-1045. DOI 10.1007/BF00224536.
25. Peterson R.F., Campbell A.B., Hannah A.E. A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can. J. Res. 1948;26(5):496-500. DOI 10.1139/cjr48c-033.
26. Prins R., Groenewald J.Z., Marais G.F., Snape J.W., Koebner R.M.D. AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat. Theor. Appl. Genet. 2001;103(4):618-624. DOI 10.1007/PL00002918.
27. Prins R., Marais G.F., Janse B.J.H., Pretorius Z.A., Marais A.S. A physical map of the Thinopyrum – derived Lr19 translocation. Genome. 1996;39:1013-1019. DOI 10.1139/g96-126.
28. Roelfs A.P., Singh R.P., Saaru E.E. Rust Diseases of Wheat: Concepts and Methods of Disease Management. Mexico, D.F.: CIMMYT, 1992. Saintenac C., Zhang W., Salcedo A., Rouse M.N., Trick H.N., Akhunov E., Dubcovsky J. Identification of wheat gene Sr35 that confers resistance to Ug99 stem rust race group. Science. 2013;341(6147): 783-786. DOI 10.1126/science.1239022.
29. Sibikeev S.N., Druzhin A.E. Prebreeding research of near-isogenic lines of spring bread wheat with a combination of translocations from Agropyron elongatum (Host.) P.B. and Aegilops ventricosa Tausch. Russ. J. Genet. Appl. Res. 2016;6:338-343. DOI 10.1134/S2079059716030126.
30. Sibikeev S.N., Druzhin A.E., Andreeva L.V. Influence analysis of combination 7DS-7DL-7AE#1L+1BL-1RS translocations on spring wheat yield and grain quality. Uspekhi Sovremennogo Estestvoznaniya = Advances in Current Natural Sciences. 2018;6:49-53. DOI 10.17513/use.36780. (in Russian)
31. Sibikeev S.N., Druzhin A.E., Badaeva E.D., Shishkina A.A., Dragovich A.Y., Gultyaeva E.I., Kroupin P.Y., Karlov G.I., Khuat T.M., Divashuk M.G. Comparative analysis of Agropyron intermedium (Host) Beauv 6Agi and 6Agi2 chromosomes in bread wheat cultivars and lines with wheat-wheatgrass substitutions. Russ. J. Genet. 2017;53(3):314-324. DOI 10.1134/S1022795417030115.
32. Sibikeev S.N., Konkova E.A., Salmova M.F. Characteristic of the bread wheat leaf rust pathogen virulence in the Saratov region conditions. Agrarnyy Nautchnyy Zhurnal = Agrarian Scientific Journal. 2020;9:40-44. DOI 10.28983/asj.y2020i9pp40-44. (in Russian)
33. Singh D., Park R.F., McIntosh R.A., Bariana H.S. Characterisation of stem rust and stripe rust seedling resistance genes in selected wheat cultivars from the United Kingdom. J. Plant Pathol. 2008;90(3): 553-562.
34. Singh R.P., Huerta-Espino J., Rajaram S., Crossa J. Agronomic effects from chromosome translocations 7DL.7AG and 1BL.1RS in spring wheat. Crop Sci. 1998;38:27-33. DOI 10.2135/cropsci1998.0011183X003800010005x.
35. Stakman E.C., Stewart D.M., Loegering W.Q. Identification of physiologic races of Puccinia graminis var. tritici. U. S. Dept. Agric. Res. Serv. 1962;E-617 (rev.):1-53.
36. The T.T., Latter B.D.H., McIntosh R.A., Ellison F.W., Brennan P.S., Fisher J., Hollamby G.J., Rathjen A.J., Wilson R.E. Grain yields of near-isogenic lines with added genes for stem rust resistance. In: Miller T.E., Koebner R.M.D. (Eds.) Proc. 7th Int. Wheat Genet. Symp. Cambridge, UK. 13–19 July. 1988., Cambridge: Inst. Plant Sci. Res., 1988;901-906.
37. Yu L.X., Liu S., Anderson J.A., Singh R.P., Jin Y., Dubcovsky J., Brown-Guidera G., Bhavani S., Morgounov A., He Z., Huerta-Espino J., Sorrells M.E. Haplotype diversity of stem rust resistance loci in uncharacterized wheat lines. Mol. Breed. 2010;26:667-680. DOI 10.1007/s11032-010-9403-7.
38. Zhang W., Olson E., Saintenac C., Rouse M., Abate Z., Jin Y., Akhunov E., Pumphrey M., Dubcovsky J. Genetic maps of stem rust resistance gene Sr35 in diploid and hexaploid wheat. Crop Sci. 2010; 50:2464-2474. DOI 10.2135/cropsci2010.04.0202/.
Review
Views:
731
Email this article 