Kazakhstan-Siberian spring common wheat identification according to glutenin and gliadin composition

Within ten years, the Kazakhstan-Siberian nursery net for spring common wheat improvement
(KASIB) working under the auspices of CIMMYT (Intenational Maize and Wheat Improvement Center) characterized accessions of five cultivar blocks with regard to the compositions of gliadin (1B/1R translocation), glutenin HMW and LMW subunits, and grain hardness. HMW glutenin compositions for 188 homogeneous variety samples are given in accordance with UPOV (Union for the Protection of New Varieties of Plants) regulations and 27, 28 and 29 features in tests for uniformity, distinctness, and stability. Most spring wheat cultivars possess the glutenin spectra 2* 7+9 5+10” (40 %), “2* 7+9 2+12” (up to 30 %), and “17+95+10” (8 %). The Kazakhstan varietal gene pool includes genotypes with a new subunit 4 + 10 on 1D in samples of Erythrospermum 55/94-01-20 and Phyton 41. Cultivar Iridost is noted as carrying the relatively rare allele 5,5 + 10 on chromosome 1D. This allele was previously identified in Tselinogradka, Tselinnaya 24, and Akmola 3 cultivars. The rare subunit 7*+ 8 is typically present in E-607 and E-757. The last three KASIB blocks (8-9; 10-11; 12-13) show higher levels of uniformity: up to 74–84 %. The 1BL.1RS wheat-rye translocation is more frequently identified in accessions by Siberian Scientific Research Institute of Agriculture and Eastern Kazakhstan Research Institute of Agriculture breeding in each KASIB block and also in all the accessions presented by the companies Kurgansemena and Agrosemkonsal and selected by the Fiton company. Varieties Altaiskaya 105, Kurganskaya 5, Lutescens 1300, Phyton 42, Lutescens 53/95-98-1, Lutescens 53/88-94-12, Lutescens 54; Lutescens 30-94, Erythrospermum 607, and Aktobe 1574 showed the whole range of variability in grain hardness (from middle soft to hard) under different growth conditions.