Экспрессия хлоропластного генома: современные представления и экспериментальные пути изучения

Уникальным свойством растений является наличие, кроме генома ядра, двух внеядерных геномов в хлоропластах и митохондриях. Геном хлоропластов относительно невелик – 100–120 генов, которые кодируют менее 5 % всех необходимых для функционирования пластид белков. Экспрессия генома пластид сохраняет черты прокариот: котранскрипцию генов в составе оперона, сходные c бактериями РНК-полимеразы и промоторы, присутствие 70S рибосом, однако появляются и новые свойства: транскрипция, не сопряженная c трансляцией, фагоподобные РНК-полимеразы, РНК эдитинг и сплайсинг транскриптов. Взаимодействие ядра (генома ядра) и цитоплазмы (генома пластид, митохондрий) в процессе развития растительного организма абсолютно необходимо для полноценного развития растения, адаптации (пластичности) к факторам окружающей среды. В обзоре обобщены современные представления об особенностях экспрессии генома пластид в клетке. Последовательно показано, что происходит при реализации генетической информации генома пластид в хлоропластах (транскрипции, эдитинге, сплайсинге, полиаденилировании, трансляции) и как отсутствие каких-либо компонентов отражается на функционировании растительной клетки и растения в целом. Описаны современные подходы к изучению пула транскриптов, выявлены критические точки ядерно-цитоплазматического взаимодействия при реали­зации функции хлоропластов в онтогенезе, воздействии факто­ров окружающей среды и др. Подробно представлена информация о важнейших факторах ядерно-цитоплазматического сигналинга у высших растений – сигма-факторах и PPR-белках, кодируемых ядром. Таким образом, показаны многоуровневость и целесообразность регуляции процессов экспрессии генома пластид в растительной клетке и взаимозависимость происходящих в разных компартментах клетки процессов. Составлена также сводка последних работ по изучению экспрессии генома пластид с помощью генетических чипов (микро- и макроэррей). Приводятся результаты собственных исследований.

хлоропласт, пластиды, экспрессия, транскрипция, РНК-полимеразы, эдитинг, сплайсинг, трансляция, микроэррей, макроэррей

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