Анализ экспрессии микроРНК и днкРНК в висцеральной жировой ткани у лиц с ожирением и без ожирения

Длинные некодирующие РНК (lncRNA) и микроРНК (miRNA) играют важную роль во всех биологических процессах, включая адипогенез, липидный обмен и инсулиновый ответ. Анализ экспрессии lncRNA и miRNA в висцеральной жировой ткани человека может способствовать более глубокому пониманию их роли в развитии метаболических нарушений. Исследование направлено на изучение уровней экспрессии lncRNA (ASMER1, SNHG9, P5549, P19461 и GAS5) и miRNA (miR-26A, miR-222, miR-221 и miR-155) в висцеральной жировой ткани у людей с абдоминальным ожирением (n = 70) по сравнению с уровнями их экспрессии у лиц без абдоминального ожирения (n = 31) методом количественной ПЦР в реальном времени. Среди протестированных miRNA уровень экспрессии miR-26A был снижен в висцеральной жировой ткани у людей с ожирением. Среди изученных lncRNA GAS5 показал значительное повышение уровня экспрессии у пациентов с ожирением и сахарным диабетом 2-го типа (СД2) (n = 10) по сравнению с лицами с ожирением без СД2 (n = 60). Уровни экспрессии GAS5 показали слабую отрицательную корреляцию (p < 0.05, r_s = 0.25) с уровнями miR-155 только у пациентов с ожирением. Положительная корреляция (p < 0.01, r_s = 0.92) между SNHG9 и GAS5 была обнаружена в группе людей без ожирения, с уменьшением коэффициента корреляции у пациентов с ожирением (p < 0.01, r_s = 0.67). Кроме того, уровни miR- 26A и miR-155 умеренно коррелировали в группе без ожирения (p < 0.05, r_s = 0.47) и демонстрировали слабую корреляцию у пациентов с ожирением (p < 0.05, r_s = 0.26). Наши результаты показали, что у пациентов с абдоминальным ожирением наблюдаются повышенные уровни экспрессии miR- 26A в висцеральной жировой ткани и слабая корреляция между экспрессией GAS5 и SNHG9 по сравнению с лицами без абдоминального ожирения.

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