Mitochondrial DNA data allow distinguishing the subpopulations in the widespread Demoiselle crane (Anthropoides virgo)

The polymorphism of the mtDNA cytochrome b (cyt b) gene’s partial sequences has been studied in the Demoiselle crane (Anthropoides virgo Linnaeus, 1778) for the first time. Based on cyt b variability, the population genetic structure of the species was characterized within most of its range in Russia. Among 157 individuals we identified 18 haplotypes, nine of which were unique. In the European samples, we observed greater haplotype and nucleotide diversity and stronger genetic differentiation than in the Asian ones. Gene flow between different parts of the Demoiselle crane range is probably mediated by birds breeding in the Trans-Urals. The overall genetic subdivision of the species as estimated by F_ST was 0.265 (p < 0.001). The structure of the gene pool is formed by three main haplotypes, one of which predominates in the Azov-Black Sea region, the second in the Caspian and Volga-Ural regions, and the third is most common in the Asian samples. Based on the correspondence of intraspecific genetic differentiation of the Demoiselle cranes from different parts of the range to their flyways, we propose to distinguish the following subpopulations: (1) Azov-Black Sea/Chadian; (2) Caspian/Sudanese; (3) Trans-Ural/Indian; (4) South Siberian/Indian; (5) Baikal/Indian and (6) Trans-Baikal/Indian. The obtained data create the basis for monitoring the genetic diversity of the Demoiselle crane and developing a scientific background for measures to protect the gene pool of the species as a whole and its subpopulations.

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