Полные последовательности пластомов видов Lonicera L.: значение для филогении и сравнительный анализ

Род Lonicera L. – один из крупнейших и экономически значимых в семействе Caprifoliaceae Juss., таксономия которого остается спорной. С целью внесения вклада в молекулярную таксономию данного рода мы секвенировали пластомы видов Lonicera – L. caerulea (два подвида), L. tatarica и L. micrantha – с использованием технологии секвенирования нового поколения и провели сравнительный анализ. Размеры пластомов варьировали от 153 985 п. н. у L. micrantha до 164 000 п. н. у L. caerulea subsp. pallasii; каждый пластом содержал 130 генов, включая 85 генов, кодирующих белок, 37 тРНК и 8 рРНК генов. Пять белок-кодирующих (rps7, rps12, ndhB, ycf2 и ycf15), 7 тРНК (trnA-UGC, trnI-CAU, trnI-GAU, trnL-CAA, trnN-GUU, trnR-ACG и trnV-GAC) и 4 рРНК (rrn4.5, rrn5, rrn16 и rrn23) гена были дуплицированы. Сравнительный анализ границ пластомов у видов Lonicera выявил структурные вариации у L. caerulea subsp. altaica и L. caerulea subsp. pallasii, особенно в распределении гена ndhA. Были идентифицированы три высоковариабельные области: две межгенные (ycf1-trnN-GUU и trnN-GUUndhF) и одна генная (accD). В четырех пластомах обнаружено всего 641 простая повторяющаяся последовательность (SSR). Филогенетический анализ сгруппировал образцы Lonicera в два клада, соответствующих подродам Periclymenum и Chamaecerasus. В настоящем исследовании впервые секвенированы пластидные геномы двух подвидов L. caerulea и вида L. micrantha. Древо, построенное методом maximum likelihood на основе полных последовательностей пластомов, оказалось наиболее информативным и демонстрировало топологию, согласующуюся с предыдущими исследованиями. Нуклеотидные последовательности вариабельных регионов (accD-ycf1-ndhF-trnN-GUU) обладают высоким потенциалом для использования в ДНК-баркодировании и могут служить ценными молекулярными маркерами для филогенетических исследований видов внутри рода Lonicera.

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