Генная сеть и база знаний по терморегуляции организма человека

   Реконструкция и анализ генных сетей, регулирующих биологические процессы, – один из эффективных подходов к исследованию сложных систем обеспечения жизнедеятельности организмов. Терморегуляция – важ­ное эволюционное приобретение человека и других теплокровных животных. Терморегуляция осуществляется при участии многих физиологических систем организма (нервной, сердечно­-сосудистой, эндокринной, дыхательной, мышечной и т. д.), что способствует поддержанию относительно постоянной температуры тела в условиях колебания температуры окружающей среды.

   Цель работы – компьютерная реконструкция генной сети терморегуляции че­ловека и представление полученных результатов в соответствующей базе знаний Termo_Reg_Human 1.0.

   Генная сеть реконструирована с использованием программно­-информационной системы ANDSystem, предназначенной для автоматизированного извлечения знаний и фактов из текстов научных публикаций и баз данных биомедицинской направленности, основанной на методах машинного обучения и искусственного интеллекта. База знаний Termo_Reg_Human 1.0 (https://www.sysbio.ru/ThermoReg_Human/) содержит информацию о генной сети терморегуляции человека, включая описание 469 генов, 473 белков и 265 микроРНК, значимых для ее функционирования; взаимодействиях между этими объектами, а также эволюционные характеристики генов. С использованием программного инструмента AND-Visio (модуля системы AND-System) проведена приоритизация каждого гена, белка и микроРНК, участвующих в терморегуляции организма человека по их функциональной нагруженности – количеству связей с другими объектами реконструированной генной сети. Установлено, что к числу ключевых объектов, имеющих наи­большее количество функциональных связей в генной сети терморегуляции человека, относятся гены UCP1, VEGFA, PPARG, DDIT3, белки STAT3, JUN, VEGFA, TLR4, TNFA и микроРНК hsa­mir­335 и hsa­mir­26b. Обнаружено обогащение генной сети терморегуляции генами, предковые варианты которых сформировались на эволюционных этапах по­явления одноклеточных организмов и дивергенции позвоночных.

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