Поиск генов домашнего хозяйства для анализа изменения экспрессии отдельных генов у Macaca mulatta

Макаки резус (Macaca mulatta) являются наиболее распространенными нечеловекообразными приматами, их используют в качестве модельных объектов, в первую очередь, из-за филогенетической и физиологической близости к человеку. В настоящее время модельные организмы широко используются для целого ряда исследований, в том числе на уровне транскриптома. При этом для анализа экспрессии отдельных генов применяется универсальный метод – полимеразная цепная реакция в реальном времени. Проведение такого рода исследований всегда требует предварительного подбора «генов домашнего хозяйства» (ГДХ) – генов, необходимых для реализации основных функций в клетке и стабильно экспрессирующихся в различных типах клеток и при разных условиях. На сегодняшний день для макак резус существуют лишь две систематизированные работы по поиску ГДХ, однако эти исследования проводились лишь для тканей мозга и не учитывают такой важный критерий, как связь ГДХ с заболеваниями. В связи с этим в нашей работе были сформулированы ключевые критерии, учитываемые при подборе ГДХ. Проведены поиск и систематизация кандидатных ГДХ. В результате сформированы две панели перспективных ГДХ для M. mulatta: расширенная панель на 56 генов и малая панель, состоящая из восьми генов: ARHGDIA, CYB5R1, NDUFA7, RRAGA, TTC1, UBA6, VPS72 и YWHAH. Обе панели соответствуют всем критериям подбора ГДХ (не имеют псевдогенов ни у макаки, ни у человека, характеризуются стабильной и достаточной экспрессией в мозге макак резус и могут быть использованы для анализа экспрессии не только в мозге, но и в периферической крови). Однако необходимо отметить, что данные экспериментально не верифицированы и требуют проверки в лабораторных условиях.

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