POLYMORPHISM AND INTERSPECIFIC VARIABILITY OF CYTOCHROME OXYDASE SUBUNIT I (COI) GENE NUCLEOTIDE SEQUENCE IN SIBLING SPECIES of A AND B Anopheles messeae AND An. beklemishevi (DIPTERA: CULICIDAE)
Abstract
Species identification of159 individuals of the Anopheles maculipennis complex from 5 localities ofUral andSiberia was conductedbyPCR-RFLP ITS2. The DNA sequences ofa COI gene fragment were determinedin 124 individuals from the localities. The Anopheles messeae groupCOI sequences were comparedwiththose from a DNA database. VariabilityofCOI nucleotide sequences (measures H andπ) ofthe crypticspecies A andB Anopheles messeae andAn. beklemishevi was investigatedandquantitativelyestimated. It was foundthat the COI variabilitydidnot allow discrimination ofthe crypticspecies A andB Anopheles messeae. Neither didit show anygeographicregularityfor these species. It is concludedthat the COI polymorphism ofthe A andB Anopheles messeae species is common for them, as well as the chromosomal inversion polymorphism. It arose long before their divergence. The diagnosticimportance ofinterspecificITS2 variabilityis confirmed.
Keywords
Anopheles maculipennis,
messeae,
beklemishevi,
crypyptic species,
ITS2,
COI,
variability,
polymorphism
About the Authors
O. V. Vaulin
Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia
Russian Federation
Russian Federation
Yu. M. Novikov
Tomsk State University, Tomsk, Russia
Russian Federation
Russian Federation
References
1. Алтухов Ю.П., Салменкова Е.А. Полиморфизм ДНК в популяционной генетике // Генетика. 2002. Т. 38. № 9. С. 1173–1195.
2. Ваулин О.В., Новиков Ю.М. Географическая изменчивость ITS2 рДНК и COIмтДНК и криптические виды малярийного комара Anopheless messeae Fall. (Diptera: Culicidae) // Информ. вестник ВОГиС. 2010. Т. 14. № 3. С. 546–557.
3. Даниленко Н.Г., Давыденко О.Г. Миры геномов органелл. Минск: Тэхналогия, 2003. 498 с.
4. Лукашов В.В. Молекулярная эволюция и филогенетический анализ. М.: БИНОМ. Лаборатория знаний, 2009. 256 с.
5. Новиков Ю.М. Влияние ассортативного скрещивания на популяционную структуру малярийного комара Anopheles messeae // 14-й Междунар. генет. конгр. Секционные заседания. М., 1978. Ч. 1. С. 471.
6. Новиков Ю.М. Anopheles messeae – два вида in statu nascendi // Макроэволюция. Матер. 1 Всесоюз. конф. по проблемам эволюции. М.: Наука, 1984. С. 67.
7. Новиков Ю.М., Шевченко А.И. Инверсионный полиморфизм и дивергенция двух криптических форм таксона Anopheles messeae (Diptera, Culicidae) на уровне повторяющихся элементов геномной ДНК // Генетика. 2001. Т. 37. № 7. С. 915–925.
8. Шевченко А.И., Новиков Ю.М. Криптические виды Anopheless messeae (Diptera, Culicidae): хиатус и связи на молекулярно-генетическом уровне // Генетика в XXXXI веке: современное состояние и перспективы развития. III Съезд ВОГиС, Москва, 6–12 июня 2004. М.: Изд-во УРСС, 2004. С. 230.
9. Alquezar D.E., Hemmerter S., Cooper R.D., Beebe N.W. Incomplete concerted evolution and reproductive isolation at the rDNDNA locus uncovers nine crypyptic species within Anopheles longirostrtris from Papua New Guinea // BMC Evol. Biol. 2010. 10: 392. doi:10.1186/1471-2148-10-392.
10. Beebe N.W., Ellis J.T., Cooper R.D., Saul A. DNA sequence analysis of the ribosomal DNDNAITS2 region for the Anopheles punctulatus group of mosquitoes // Insect Mol. Biol�. 1999. V. 8. No .....3. P. 381–390.
11. Beebe N.W., Maung J., van den Hurk A.F. et al. Ribosomal DNA spacer genotypes of the Anopheles bancroftii group (Diptera: Culicidae) from Australia and New Guinea // Insect Mol. Biol. 2001. V. 10. No. 5. P. 407–414.
12. Bandelt H.-J., Forster P., Rohl A. Median-joining networks for inferring intraspecific phylogenies // Mol. Biol. Evol. 1999. V. 16. No. 1. P. 37–48.
13. Bender W., Spierer P., Hognes D.S., Chambon P. Chromosomal walking and jumping to isolate DNA from Ace and rosy loci of bithorax loci in Drosophila melanogaster // J. Mol. Biol. 1983. V. 168. No .....1. P. 17–33.
14. Bezzhonova O.V., Gorryacheva I.I. Intragenomic Heterogeneity of rDNDNA Internal Transcriped Spacer 2 in Anopheles messeae (Diptera: Culicidae) // J. Med. Ent. 2008. V. 45. No. 3. P. 337–341.
15. Cohuet A., Dia I., Simard F. et al. Population structure of the malaria vector Anopheles funestus in Senegal based on microsatellite and cycytogenetic data // Insect Mol. Biol. 2004. V. 13. No .....3. P. 251–258.
16. Dover G., Flavell R. Molecular coevolution: DNDNA divergence and the maintenance of function // Cell. 1984. V. 38. Issue. 3. P. 622–623.
17. Fields B.N., Knipe D.M., Howley P.M. et al. Fields Virology. Pliladelphia: Wolters Kluwer Health/Lippincott Williams andWilkins. 2007. 317 p.
18. Harbachch R.E. The classification of genus Anopheles (Diptera: Culicidae): a working hyphyphypothesis of phyphyphylogenetic relationships // Bull. Entomol. Res. 2004. V. 94. No. 6. P. 537–553.
19. Jukes T.H., Cantor T.R. Evolution of protein molecules // Mammalian protein metabolism / Ed. H.N. Munro. N.Y., USA: Acad. Press, 1969. P. 21–123.
20. Kumar A., Rai K.S. Chromosomal localization and copypy number of 18s + 28s ribosomal RNA genes in evolutionarily diverse mosquitoes (Diptera, Culicidae) // Hereditas. 1990. V. 113. No .....3. P. 277–289.
21. Michel A.P., Guelbeogo W.M., Grushko O. et al. Molecular differentiation between chromosomally defined incipient species of Anopheles funestus // Insect Mol. Biol. 2005. V. 14. No 4. P. 375–387.
22. Marinucci M., Romi R., Mancini M. et al. Phylogenetic relationships of seven palearctic members of the maculipennis complex inferred from ITS2 sequence analysis // Insect. Mol. Biol. 1999. V. 8. No 4. P. 469–480.
23. Nei M. Analysis of gene diversity in subdivided populations // Proc. Natl Acad. Sci. USA. 1973. V. 70. No.12. P. 3321–3323.
24. Nicolescu G., Linton Y.-M., Vladimirescu A. et al. Mosquitoes of the Anopheles maculipennis group (Diptera: Culicidae) in Romania, with the discovery and formal recognition of a new species based on molecular and morphphological evidence // Bull. Ent. Res. 2004. V. 94. No. 6. P. 525–535.
25. Tamura K., Dudley J., Nei M., Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0 // Mol. Biol. Evol. 2007. V. 24. No�.8. P. 1596–1599.
26. della Torre A., Fanello C., Akogbeto M. et al. Molecular evidence of incipient speciation within Anopheles gambiae s.s. in West Africa // Insect. Mol. Biol. 2001. V. 10. No. 1. P. 9–18.
27. Wilkerson R.C., Reinert J.F., Li C. Ribosomal DNDNA ITS2 sequences difffferentiation six species in the Anopheles crucians complex (Diptera: Culicidae) // J. Med. Entomol. 2004. V. 41. N.3. P.392-401.
Review
Views:
618
Email this article 