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Study of nodulation and nitrogen fixation in two cowpea [Vigna unguiculata (L.) Walp.] cultivars inoculated with different strains of Bradyrhizobium sp.

https://doi.org/10.18699/VJ16.099

Abstract

Cowpea (Vigna unguiculata L. Walp.) is a vegetable legume with promise for agricultural production in Russia. The impact of inoculation with three strains of Bradyrhizobium sp. from the All Russian Institute of Agricultural Microbiology (St. Petersburg) on nodulation and nitrogen fixation on two cowpea cultivars, Sibirskiy razmer and Yunnanskaya, has been explored All the strains used made both cultivars produce nitrogen fixing nodules. Differences between the varieties in the ability to form nodules and fix nitrogen following exposure to the different strains have been identified. High variation of the nodulation ability of both cultivars has been observed: at the beginning of flowering, the number of nodules per plant was 4–47 in Sibirskiy razmer and 17–117 in Yunnanskaya. Uninoculated vigna roots used as the control did not form nodules. At the beginning of flowering (48 days after inoculation) Sibirskiy razmer plants inoculated with strain 164 0503 (03) had the highest nodule weight per plant (0.79 g) and N2 fixation rates (5155.3 nmol С2H4/plant/h). The corresponding measures in Yunnanskaya were 1.41 g and 5255.5 nmol С2H4/plant/h following exposure to strain 162 0501 (01) and 4673.0 nmol С2H4/plant/h following exposure to strain (03). Analysis showed a correlation between nitrogen fixation rate and nodule weight (pcs./plant), r = 0.78 (p > 0.95). Data obtained suggest that effective symbioses are achieved between Sibirskiy razmer and strain 03 as well as between Yunnanskaya and strain 01.

About the Authors

Yu. V. Fotev
Central Siberian Botanical Garden SB RAS
Russian Federation
Novosibirsk


K. K. Sidorova
Institute of Cytology and Genetics SB RAS
Russian Federation
Novosibirsk


T. I. Novikova
Central Siberian Botanical Garden SB RAS
Russian Federation
Novosibirsk


V. P. Belousova
Central Siberian Botanical Garden SB RAS
Russian Federation
Novosibirsk


References

1. Agbenin J.O., Lombin G., Owonubi J.J. Effect of boron and nitrogen fertilization on cowpea nodulation, mineral nutrition and grain yield. Fertilizer Res. 1990;22(2):71- 78.

2. Awonaike K.O., Kumarasinghe K.S., Danso S.K.A. Nitrogen fixation and yield of cowpea (Vigna unguiculata) as influenced by cultivar and Bradyrhizobium strain. Field Crops Res. 1990;24(3-4):163-171.

3. Dakora F.D., Belane A.K., Mohale K.C., Makhubkdu T.I., Makhura P., Pule-Meulenberg F., Mapope N., Mogkelhe S.N., Gyoglluu C., Phatlane G.P., Muhaba S., Mokobane F., Oteng-Frimpong R. Food Grain Legumes: Their Contribution to Soil Fertility, Food Security and Human Nutrition/Health in Africa. Biological Nitrogen Fixation. Eds F.J. de Bruijn. John Wiley and Sons, Inc., 2015;2(Ch. 105): 1063-1070.

4. Dragovoz I.V., Leonova N.O., Iutinskaya G.A. The synthesis of phytohormones by strains of Bradyrhizobium japonicum with different symbiotic efficiencies. Mіkrobіol. Zhurn. = Microbiology Journal. 2011;73(4):29-35.

5. Fotev Yu.V., Belousova V.P. Cowpea. Introduktsiya netraditsionnykh plodovykh, yagodnykh i ovoshchnykh rasteniy v Zapadnoy Sibiri. Otv. red. I.Yu. Koropachinskiy, A.B. Gorbunov [The Introduction of Nontraditional Fruit, Berry, and Vegetable Plants in West Siberia. Eds I.Yu. Koropachinsky, A.B. Gorbunov]. Novosibirsk, Geo Publ., 2013.

6. Fotev Yu.V., Kudryavtseva G.A., Belousova V.P. Biological features and productivity of cow pea under conditions of Siberia. Sibirskiy Vestnik Selskokhozyaystvennoy Nauki = Siberian Herald of Agricultural Sciences. 2007;4:32-36.

7. Gómez C. Cowpea: Post-Harvest Operations. Ed. D. Mejía. FAO, Rome (Italy). 2004.

8. Gosudarstvennyy reestr selektsionnykh dostizheniy, dopushchennykh k ispolzovaniyu (po sostoyaniyu na 09.04.2015 g.) [State Register of Breeding Achievements Approved for Usage as of April 09, 2015].Available at: http://www.gossort.com/ree_cont.html (accessed August 20, 2015).

9. Kahn B.A., Stoffella P.J. Nodule distribution among root morphological components of field-grown cowpeas. J. Amer. Soc. Hort. Sci. 1991;116(4):655-658.

10. Keyser H.H., Berkum P.V., Weber D.F. A comparative study of the physiology of symbioses formed by Rhizobium japonicum with Glycine max, Vigna unguiculata, and Macroptilium atropurpureum. Plant Physiol. 1982;70:1626-1630.

11. Krutylo D.F. Funktsіonuvannya simbіotichnoї sistemi vigna kitayska – bulbochkovі bakterії [The functioning of symbiotic system: cowpea − nodule bacteria]. Sіlskogospodarska mіkrobіologіya: mіzhvіdomchiy tematichniy naukoviy zbіrnik [Agricultural Microbiology: Interdepartmental Thematic Collection of Scientific Articles]. Chernigov, TsNP Publ. 2010;12:46-58.

12. Marandu A.E.T., Semu E., Mrema J.P., Nyaki A.S. Quantification of atmospheric N2 fixed by cowpea, pigeonpea and greengram grown on Ferralsols in Muheza District, Tanzania. Tanzania J. Agric. Sci. 2010;10(1):28-37.

13. Naumova N.B., Fotev Yu.V., Bugrovskaya G.A., Belousova V.P. Macro-and micronutrient composition of greenhouse-grown cowpea, kiwano, bitter melon and wax gourd. Ovoshchi Rossii = Vegetables of Russia. 2014;3(24):11-17.

14. Neves M.C.P, Ramos M.L.G., Martinazzo A.F., Botelho G.R., Doberiener J. Adaptation of more efficient soybean and cowpea rhizobia to replace established populations. Biological nitrogen fixation and sustainability of tropical agriculture: Proc. of the the 4th Intern. Conf. of the African Association for Biological Nitrogen Fixation (AABNF), Int. Inst. Tropical Agric., Nigeria. Sept. 24–28, 1990:219-233.

15. Omel’yanuk L.V., Sidorova K.K., Shumny V.K. Study of nodulation and nitrogen fixation in introduced cultivars and candidate lines of pea (Pisum sativum L.) grown at two nitrogenous nutrition levels. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2013;17(3):424-429.

16. Omel’yanuk L.V., Sidorova K.K., Shumny V.K. Study of nodulation and nitrogen fixation in introduced cultivars and candidate lines of pea (Pisum sativum L.) grown at two nitrogenous nutrition levels. Russ. J. Genet.: Appl. Res. 2014;4(1):19-22.

17. Pavlova A.M. The value of black-eyed cowpea for breeding. Trudy po prikladnoy botanike, genetike i selektsii = Proceedings on Applied Botany, Genetics and Breeding. 1959;32(3):228-232.

18. Piennar B.J., van Wyk A.E. The Vigna unguiculata complex (Fabaceae) in South Africa. South Afr. J. Bot. 1992;58:414.

19. Pule-Meulenberg F., Alphonsus K., Belane A.K., Krasova-Wade T., Dakora F.D. Symbiotic functioning and bradyrhizobial biodiversity of cowpea (Vigna unguiculata L. Walp.) in Africa. BMC Microbiol. 2010;10:1-12. DOI 10.1186/1471-2180-10-89

20. Pule-Meulenberg, F., Dakora, F.D. Nodule Functioning and Symbiotic Efficiency of Cowpea and Soybean Varieties in Africa. Biological Nitrogen Fixation. Ed. F.J. de Bruijn. John Wiley and Sons, Inc, Hoboken, NJ (USA). 2015;2:1025-1030. DOI 10.1002/9781119053095.ch100

21. Semenova I.V. Study of the capacity of Bradyrhizobium japonicum strains for symbiosis with Vigna unguiculata. Tezisy dokladov VI Molodezhnoy shkoly-konferentsii s mezhdunarodnym uchastiem «Aktualnye aspekty sovremennoy mikrobiologii», 25–27 oktyabrya 2010 g. [Abstracts from the 6th International Youth School-Conference “Current issues of modern microbiology”, October 25–27, 2010]. Moscow, 2010.

22. Senaratne R., Ratnasinghe D.S. Ontogenic variation in nitrogen fixation and accumulation of nitrogen in mungbean, blackgram, cowpea, and groundnut. Biology Fert. Soils. 1993;16(2):125-130.

23. Serova T.A., Tsyganov V.E. Symbiotic nodule senescence in legumes: molecular-genetic and cellular aspects (review). Selskokhozyaystvennaya Biologiya = Agricultural Biology. 2014;5:3-15.

24. Singh B.B. Cowpea: the food legume of the 21th century. Madison, WI., USA. 2014. DOI 10.2135/2014.cowpea URL: http://dx.doi.org/10.2135/2014.cowpea

25. Singh B.B., Chambliss O.L., Sharma B. Recent advances in cowpea breeding. Advances in cowpea research. Eds B.B. Singh, D.R. Mohan, K.E. Dashiell, L.E.N. Jackai. Copublication of International Institute of Tropical Agriculture (IITA) and Japan International Research Center for Agricultural Sciences (JIRCAS). IITA. Ibadan, Nigeria. 1997:30-49.

26. Vishnyakova M.А., Aleksandrova T.G., Buravtseva T.V., Bulyntsev S.V., Burlyaeva M.O., Egorova G.P., Semenova E.V., Seferova I.V., Yankov I.I. Strategy and tactics of the mobilization of grain legume genetic resources in the collection of the All- Russia Plant Breeding Institute at the turn of the century. Trudy po prikladnoy botanike, genetike i selektsii = Proceedings on Applied Botany, Genetics, and Breeding. 2012;169:41-52.

27. Wadisirisuk P., Weaver R.W. Importance of bacteroid number in nodules and effective nodule mass to dinitrogen fixation by cowpeas. Plant Soil. 1985;87(2):223-231.

28. Wang D., Yang S., Tang F., Hongyan Zhu H. Symbiosis specificity in the legume – rhizobial mutualism. Cell. Microbiol. 2012;14(3):334- 342. DOI 10.1111/j.1462-5822.2011.01736.x

29. You Z., Marutani M., Borthakur D. Diversity among Bradyrhizobium isolates nodulating yardlong bean and sunnhemp in Guam. J. Appl. Microbiol. 2002;93(4):577-584. DOI 10.1046/j.1365-2672.2002.01733.x

30. Zaitsev G.N. Metodika biometricheskikh raschetov [Methods of Biometric Calculations]. Moscow, Nauka, 1973.


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