Study of fertility and cytogenetic variability in androgenic plants (R0 and R1) of alloplasmic introgression lines of common wheat

Anther culture is one of the methods to obtain DH lines of wheat. A limitation of this method can be cytogenetic instability in plants R0, leading to a decrease in fertility or sterility. In this study, we have investigated the fertility of R0, the fertility and cytogenetic variability of R1 in alloplasmatic introgression lines of common wheat in order to develop a cytogenetically stable DH lines with introgressions from different species. Lines 311/134, 311/FL, 311/IR with the cytoplasm from H. vulgare were studied. 311/134 carries the wheat-rye 1RS.1BL and wheatwheatgrass 7DL-7Ai translocations; 311/FL has the 1RS.1BL translocation and probably introgressions from A. glaucum; and 311/IR has the wheat-rye 1RS.1BL and wheat-Ae. speltoides T2B/2S#2 translocations. Green seedlings developed in anther culture for all lines. Differences between the lines in the ability for androgenesis and in the level of fertility in R0 and R1 have been revealed. Depressed androgenesis, low fertility and high aneuploidy were observed in 311/IR. It has been proposed that the reason for this is cytogenetic instability in gametes, which is caused by Gc genes located on T2B/2S#2. 63.3 % of 311/134 and 311/FL R1 plants that were grown from low seed-set R0 plants were aneuploids. Fertile R0 regenerant plants were identified that segregated in R1 for fertility and chromosome numbers. It has been demonstrated that DH lines are best developed from highfertility R1 plants with 2n = 42 irrespective of fertility in R0.

alloplasmic introgression lines (H. vulgare)- T. aestivum, anther culture, androgenesis, dihaploid lines, fertility, cytogenetic instability

1. Barnabas B., Szakacs Ĕ., Karsai I., Bedö Z. In vitro androgenesis of wheat: from fundamental to practical application. Euphytica. 2001; 119(1);211-216.

2. Belan I., Rosseeva L., Laikova L., Rosseev V., Pershina L., Trubacheeva N., Morgounov A., Zelenskiy Y. Utilization of new wheat genepool in breeding of spring bread wheat. [Proc. 8th Int. Wheat Conference]. St. Petersburg, 2010;69-70.

3. Belan I.A., Rosseeva L.P., Meshkova L.V., Shepelev S.S., Zelensky Yu.I. Immunological evaluation of “KASIB” material in southern forest–steppe of West Siberia. Vestnik Altayskogo Gosudarstvennogo Agrarnogo Universiteta = Bulletin of the Altai State Agricultural University. 2012b;10(96):39-43.

4. Belan I.A., Rosseeva L.P., Rosseev V.M., Badaeva E.D., Zelenskiy Y.I., Blokhina N.P., Shepelev S.S., Pershina L.A. Examination of adaptive and agronomic characters in lines of common wheat Omskaya 37 bearing translocations 1RS.1BL and 7DL-7Ai. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2012а;16(1):178-186.

5. Belan I.A., Rosseeva L.P., Rosseev V.M., Badayeva E.D., Zelensky Yu.I., Blochina N.P., Shepelev S.S., Pershina L.A. Examination of adaptive and agronomic characters in lines of common wheat Omskaya 37 bearing translocations 1RS.1BL and 7DL-7Ai. Russ. J. Genet.: Appl. Res. 2015;5(1):41-47. DOI 10.1134/S2079059715010037

6. Chuang C.C., Ouyang J.W., Chia H., Chou S.M., Ching C.K. A set of potato media for wheat anther culture. Proc. Symp. Plant Tissue Culture. Peking, 1978:51-56.

7. Forster B.P., Thomas W.T.B. Doubled haploids in genetics and plant breeding. Plant Breed. Rev. 2005;25:57-88. DOI 10.1002/9780470650301.ch3

8. Friebe B., Jiang J., Raupp W.J., McIntosh R.A., Gill B.S. Characterization of wheat- alien translocations conferring resistance to diseases and pests: current status. Euphytica. 1996;91(1):59-87.

9. Gamborg O.L., Eveleigh D.E. Culture methods and detection of glucanases in suspension cultures of wheat and barley. Can. J. Biochem. 1968;46(5):417-421.

10. Germana M.A. Gametic embryogenesis and haploid technology as valuable support to plant breeding. Plant Cell Rep. 2011;30(5):839-857. DOI 10.1007/s00299-011-1061-7

11. Gupta S.K., Charpe A., Prabhu K.V., Haque Q.M. Identification and validation of molecular markers linked to the leaf rust resistance gene Lr19 in wheat. Theor. Appl. Genet. 2006;113(6):1027-1036. DOI 10.1007/s00122-006-0362-7

12. Hu H., Huang B. Application of pollen-derived plants to crop improvement. Int. Rev. Cytol. 1987(107):293-313. DOI 10.1016/S0074-7696(08)61079-7

13. Hussain B., Khan M.A., Ali Q., Shaukat S. Double haploid production is the best method for genetic improvement and genetic studies of wheat? Int. J. Agro Veter. Med. Sci. 2012;6(4):216-228.

14. Jane A., Lorz H. Cereal microspore culture. Plant Sci. 1995;109(1):1-12.

15. Joshi R.K., Nayak S. Gene pyramiding – a broad spectrum technique for developing durable stress resistance in crop. Biotechnol. Mol. Biol. Rev. 2010;5(3):51-60.

16. Konieczny R., Czaplicki A.Z., Golczyk H., Przywara L. Two pathways of plant regeneration in wheat anther culture. Plant Cell, Tissue Organ Culture. 2003;73(2):177-187.

17. Liu S., Yu L.-X., Singh R.P., Jin Y., Sorrels M.E., Anderson J.A. Diagnostic and co- dominant PCR markers for wheat stem rust resistance genes Sr25 and Sr26. Theor. Appl. Genet. 2010;120(4):691-697. DOI 10.1007/s00122-009-1186-z

18. Liu W., Zheng M.Y., Polle E.A., Konzak C.F. Highly efficient doubledhaploid production in wheat (Triticum aestivum L.) via induced microspore embryogenesis. Crop Sci. 2002;42(3):686-692.

19. Mago R., Spielmeyer W., Lawrence J., Lagudah S., Ellis G., Pryor A. Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines. Theor. Appl. Genet. 2002;104(8):1317-1324. DOI 10.1007/s00122-002-0879-3

20. Marais G.F., Bekker T.A., Eksteen A., McCallum B., Fetch T., Marais A.S. Attempts to remove gametocidal genes co-transferred to common wheat with rust resistance from Aegilops speltoides. Euphytica. 2010;171(1):71-85. DOI 10.1007/s10681-009-9996-2

21. Nasuda S., Friebe B., Gill B.S. The morphology of the chromosome fragments suggests that the Gc genes induce chromosome breaks in the G1 phase prior to DNA synthesis of the first postmeiotic mitosis. Genetics. 1998;149(2):1115-1124.

22. Niroula R.K., Bimb H.P. Overview of wheat × maize system of crosses for dihaploid induction in wheat. World Appl. Sci. J. 2009;7(8): 1037-1045.

23. Oleszczuk S., Rabiza-Swider J., Zimny J., Lukaszewski A.J. Aneuploidy among androgenic progeny of hexaploid triticale (×Triticosecale Wittmack). Plant Cell Rep. 2011;30(4):575-586. DOI 10.1007/s00299-010-0971-0

24. Oleszczuk S., Tyrka M., Zimny J. The origin of clones among androgenic regenerants of hexaploid triticale. Euphytica. 2014;198(3): 325-336.

25. Osadchaya T.S., Pershina L.A., Trubacheeva N.V., Belan I.A., Rosseeva L.P., Devyatkina E.P. Androgenesis ability in common wheat euplasmic lines and alloplasmic recombinant lines (H. vulgare)- T. aestivum possessing 1RS.1BL and 7DL-7Ai translocations and production of doubled haploids lines.Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2014;18(4/1): 650-659.

26. Osadchaya T.S., Pershina L.A., Trubacheeva N.V., Belan I.A., Rosseeva L.P., Devyatkina E.P. Androgenesis ability in common wheat euplasmic lines and alloplasmic recombinant lines (H. vulgare)- T. aestivum possessing 1RS.1BL and 7DL-7Ai translocations and production of double haploid lines. Russ. J. Genet.: Appl. Res. 2015; 5(3):174-181. DOI 10.1134/S2079059715030132

27. Pershina L.A., Osadchaya T.S., Badaeva E.D., Belan I.A., Rosseeva L.P. Features of androgenesis in anther cultures of varieties and a promising accession of spring common wheat bred in West Siberia differing in the presence or absence of wheat- alien translocations. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2013;17(1):40-49.

28. Pershina L.A., Osadchaya T.S., Badaeva E.D., Belan I.A., Rosseeva L.P. Features of androgenesis in anther cultures of varieties and a promising accession of spring common wheat bred in West Siberia differing in the presence or absence of wheat- alien translocations. Russ. J. Genet.: Appl. Res. 2013;3(4):246-253. DOI 10.1134/S2079059713040096

29. Sibikeev S.N., Voronina S.A., Badaeva E.D., Druzhin A.E. Study of resistance to leaf and stem rusts in Triticum aestivum – Aegilops speltoides lines. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2015;19(2):165-170.

30. Sibikeev S.N., Voronina S.A., Badaeva E.D., Druzhin A.E. Study of resistance to leaf and stem rust in Triticum aestivum – Aegilops speltoides lines. Russ. J. Genet.: Appl. Res. 2016;6(4):351-356. DOI 10.1134/S2079059716040183

31. Sibikeeva Yu.E., Sibikeev S.N., Krupnov V.A. The effect of Lr19-translocation on in vitro androgenesis and inheritance of leaf-rust resistance in DH3 lines and F2 hybrids of common wheat. Genetika = Genetics (Moscow). 2004;40(9):1224-1228.

32. Sibikeeva Yu.E., Sibikeev S.N., Krupnov V.A. The effect of Lr19-translocation on in vitro androgenesis and inheritance of leaf-rust resistance in DH3 lines and F2 hybrids of common wheat. Russ. J. Genet. 2004;40(9):1003-1006.

33. Singh N.K., Shepherd K.W., McIntosh R.A. Linkage mapping of genes for resistance to leaf, steam and stripe rust and ω-secalins on the short arm of rye chromosome 1R. Theor. Appl. Genet. 1990;80:609-616. DOI 10.1007/BF00224219. pmid:24221066

34. Sinha P., Tomar S.M., Vinod, Singh V.K., Balyan H.S. Genetic analysis and molecular mapping of a new fertility restorer gene Rf8 for Triticum timopheevi cytoplasm in wheat (Triticum aestivum L.) using SSR markers. Genetica. 2013;141(10-12):431-341. DOI 10.1007/s10709-013-9742-5

35. Stepochkin P.I., Ponomarenko V.I., Pershina L.A., Osadchaya T.S., Trubacheeva N.V. Utilization of distant hybridization for development of breeding material of winter wheat. Dostizheniya nauki i tekhniki APK = Achievements of Science and Technology of AIC. 2012;6:37-38.

36. Tersi M., Xynias I.N., Gouli-Vadinoudi E., Roupakias D.G. Anther culture response of F1 durum bread wheat hybrids after colchicines. Plant Breed. 2006;125(5):457-460. DOI 10.1111/j.1439-0523.2006.01285.x

37. Veilleux R.E. Gametoclonal variation in crop plants. Current Plant Sci. Biotechnol. Agricult. 1998;32;123-133.