MODELING OF PLANT EMBRYO MORPHODYNAMICS AT EARLY DEVELOPMENTAL STAGES

Embryo morphodynamics at early developmental stages of Arabidopsis thaliana was studied. First, a pipeline was elaborated from confocal microscopy and tissue 3D reconstruction to cell lineage tree reconstruction and numerical simulation of growing embryo mechanics. Tentative results of its use are presented.

1. Болотин В.В., Новичков Ю.Н. Механика многослойных конструкций. М.: Машиностроение, 1980.

2. Голушко С.К., Немировский Ю.В. Прямые и обратные задачи механики упругих композитных пластин и оболочек вращения. М.: Физматлит, 2008.

3. Гонсалес Р., Вудс Р. Цифровая обработка изображений. М.: Техносфера, 2006.

4. Препарата Ф., Шеймос М. Вычислительная геометрия: Введение. М.: Мир, 1989.

5. Bao Z., Murray J.I., Boyle T. et al. Automated cell lineage tracing in Caenorhabditis elegans // Proc. Natl Acad. Sci. USA. 2006. V. 103. P. 2707–2712.

6. Buckingham M.E., Meilhac S.M. Tracing cells for tracking cell lineage and clonal behavior // Developm. Cell. 2011. V. 21. P. 394–409.

7. Chanliaud E., Burrows K.M., Jeronimidis G., Gidley M.J. Mechanical properties of primary plant cell wall analogues // Planta. 2002 V. 215. P. 989–996.

8. Fayant P., Girlanda O., Chebli Y. et al. Finite element model of polar growth in pollen tubes // Plant Cell. 2010. V. 22. P. 2579–2593.

9. Fernandez R., Das P., Mirabet V. et al. Imaging plant growth in 4D: robust tissue reconstruction and lineaging at cell resolution // Nat. Meth. 2010. 7. P. 547–553.

10. Handbook of Biological Confocal Microscopy / Ed. J.B. Pawley. Springer, N.Y. 2nd ed. 2006. 988 p.

11. Kretzschmar K., Watt F.M. Lineage Tracing // Cell. 2012. 148. P. 33–45.

12. Laux T., Würschum T., Breuninger H. Genetic regulation of embryonic pattern formation // Plant Cell. 2004. V. 16. Suppl. 1. P. S190–S202.

13. Olivier N., Luengo-Oroz M.A., Duloquin L. et al. Cell lineage reconstruction of early zebrafi sh embryos using label-free nonlinear microscopy // Science. 2010. V. 329. P. 967–971.

14. Truernit E., Bauby H., Dubreucq B. et al. High-resolution whole-mount imaging of three-dimensional tissue organization and gene expression enables the study of phloem development and structure in Arabidopsis // Plant Cell. 2008. V. 20. P. 1494–1503.