The creation of new aneuploid lines of the cotton (Gossypium hirsutum L.) with identification of chromosomes by translocation and SSR-markers
https://doi.org/10.18699/VJ16.186
Abstract
The use of monosomic lines significantly increases the effectiveness of molecular-genetic analysis and the development of superior quality breeding lines via substitutions by alien chromosomes. A complete set of aneuploid series for each cotton chromosome was required. Simple sequence repeats (SSR) have been applied as useful markers for understanding cotton genetics. Several DNA markers have already been assigned to the individual chromosomes of Gossypium hirsutum. The primary objective of this paper is to report the creation of new monosomic lines and the use of chromosome specific simple sequence repeat (SSR) markers and translocation lines for chromosome identification to confirm chromosome specificity of monosomic lines in the Uzbek collection. Here, we summarize data on the development of a monosomic stock collection of cotton (G. hirsutum L.) from Uzbekistan, including the origin of 95 primary monosomics, their cytogenetic characteristics, morphological features and their identification by translocation tests and chromosome SSR-markers. Our results indicated several different monosomic lines identified by chromosomes 2, 4, 6 and telosome 11 of the At subgenome and chromosomes 18 and 22 of the Dt subgenome, 22 monosomes were identified as duplicates of three monosomes (chromosomes 2, 4 and 6). Chromosome 4 of the At subgenome was recovered more frequently (18 times) than chromosome 2 of the At subgenome (4 times) and chromosome 6 of the At subgenome (3 times) during pollen irradiation at different doses and in the progeny of the desynaptic plants. These lines will be useful for molecular mapping and enhancement of Upland cotton.
About the Authors
M. F. Sanamyan
National University of Uzbekistan named M. Ulugbek
Uzbekistan
Tashkent, Uzbekistan
Uzbekistan
Tashkent, Uzbekistan
Sh. U. Bobokhujaev
National University of Uzbekistan named M. Ulugbek
Uzbekistan
Tashkent, Uzbekistan
Uzbekistan
Tashkent, Uzbekistan
A. X. Makamov
Center of Genomics and Bioinformatics UAS
Uzbekistan
Tashkent, Uzbekistan
Uzbekistan
Tashkent, Uzbekistan
S. G. Achilov
Center of Genomics and Bioinformatics UAS
Uzbekistan
Tashkent, Uzbekistan
Uzbekistan
Tashkent, Uzbekistan
I. Y. Abdurakhmonov
Center of Genomics and Bioinformatics UAS
Uzbekistan
Tashkent, Uzbekistan
Uzbekistan
Tashkent, Uzbekistan
References
1. Abdurakhmonov I.Y., Buriev Z.T., Saha S., Pepper A.E., Musaev J.A., Almatov A., Shermatov S.E., Kushanov F.N., Mavlonov G., Reddy U.K., Yu J.Z., Jenkins J.N., Kohel R.J., Abdukarimov A. Microsatellite markers associated with lint percentage trait in cotton, Gossypium hirsutum. Euphytica. 2007;156:141-156.
2. Blenda A., Fang D.D., Rami J.F., Garsmeur O., Luo F. A high density consensus genetic map of tetraploid cotton that integrates multiple component maps through molecular marker redundancy check. PLoS ONE. 2012;7(9):e45739. DOI 10.1371/journal.pone.0045739.
3. Brown M.S. The identification of chromosomes in Gossypium hirsutum by means of translocations. Genetics. 1965;52:430-431.
4. Brown M.S. The identification of the individual chromosomes of Gossypium hirsutum L. Proc. Beltw. Cotton Prod. Res. Conf. Dallas, January 9–11 1978. Dallas, 1978;70.
5. Brown M.S. Identification of the chromosomes of Gossypium hirsutumL. by means of translocations. J. Hered. 1980;71(4):266-274.
6. Brown M.S., Endrizzi J.E. The origin, fertility and transmission of monosomic in Gossypium. Amer. J. Bot. 1964;51(1):108-115.
7. Edwards G.A., Brown M.S., Niles G.A., Naqi S.A. Monosomics of cotton. Crop Sci. 1980a;20(4):527-528.
8. Edwards G.A., Brown M.S., Niles G.A., Naqi S.A. The frequency of spontaneous and other monosomes at Beasley laboratory, 1946 to 1979. Proc. Beltw. Cotton Prod. Res. Conf., 1980b;103-106.
9. Endrizzi J.E. Use of haploids in Gossypium barbadense L. as a source of aneuploids. Current Science. 1966;(2):34-35.
10. Endrizzi J.E., Brown M.S. Identification of monosomes for six chromosomes in Gossypium hirsutum. Amer. J. Botany. 1964;51:117-120.
11. Endrizzi J.E., Ramsay G. Monosomes and telosomes for 18 of the 26 chromosomes of Gossypium hirsutum. Can. J. Genet. Cytol. 1979;21(4):531-536.
12. Endrizzi J.E., Ramsay G. Identification of ten chromosome deficiencies of cotton. Cytological identification of eight chromosomes and genetic analysis of chromosome deficiencies and marker genes. J. Hered. 1980;71(1):45-48.
13. Endrizzi J.E., Ray D.T. Monosomic and monotelodisomic analysis of 34 mutant loci in cotton. J. Hered. 1991;82:53-57.
14. Endrizzi J.E., Ray D.T. Mapping of the cl1, R1, yg1 and Dw loci in the long arm of chromosome 16 of cotton. J. Hered. 1992;83:1-5.
15. Endrizzi J.E., Turcotte E.L., Kohel R.J. Genetics, cytology and evolution of Gossypium. Adv. Genet. 1985;23:271-375.
16. Galen D.F., Endrizzi J.E. Induction of monosomes and mutations in cotton by gamma irradiation of pollen. J. Hered. 1968;59(6): 343-346.
17. Hoffman S.M., Yu J.Z., Grum D.S., Xiao J., Kohel R.J., Pepper A.E. Identification of 700 new microsatellite loci from cotton (G. hirsutum L.). J. Cotton Science. 2007;11:208-241.
18. Kakani A., Saha S., Sapra V.T., Zipf A., Stelly D.M. Genetic mechanism and chromosomal location of pollen-specific gene (s) in Gossypium. Crop Sci. 1999;39:668-673.
19. Kohel R.J., Stelly D.M., Yu J. Tests of six cotton (Gossypium hirsutum L.) mutants for association with aneuploids. J. Hered. 2002; 93(2):130-132.
20. Lacape J.M., Jacobs J., Arioli T. A new interspecific, Gossipium hirsutum × G. barbadense, RIL population: Towards a unified consensus linkage map of tetraploid cotton. Theor. Appl. Cenet. 2009;119(2): 281-282. DOI 10.1007/s00122-009-1037-y.
21. Liu S., Saha S., Stelly D., Burr B., Cantrel R.G. The use of cotton aneuploid for the chromosomal assignment of microsatellite loci in cotton. J. Hered. 2000;91:326-332.
22. Musayev D.A. Geneticheskaya kollektsiya khlopchatnika [Genetic Сollection of Сotton]. Tashkent, FAN Publ., 1979. (in Russian)
23. Myles E.L., Endrizzi J.E. Aneuploids induced by deficiencies of chromosome 9 and analysis of the time of nondisjunction in cotton. Genome. 1989;32:12-18.
24. Nguyen T.B., Ciband M., Brottier P., Risterucci A.M., Lacape J.M. Wide coverage of the tetraploid cotton genome using newly developed microsatellite markers. Theor. Appl. Genet. 2004;109:167-176. DOI 10.1007/s00122-004-1612-1.
25. Rakhmatullina E.M., Sanamyan M.F. Estimation of the efficiency of seed irradiation by thermal neutrons for inducing chromosomal aberrations in M1 of cotton Gossypium hirsutum. Genetika = Genetics (Moscow). 2007a;43(4):499-507. (in Russian)
26. Rakhmatullina E.M., Sanamyan M.F. Estimation of the efficiency of seed irradiation by thermal neutrons for inducing chromosomal aberrations in M2 of cotton Gossypium hirsutum. Genetika = Genetics (Moscow). 2007b;43(5):639-646. (in Russian)
27. Reddy A.S., Connell J., Pammi S., Iqbal J. Genetic mapping of cotton: isolation and polymorphism of microsatellites. Proc. Beltw. Cotton Prod. Res. Conf., Memphis, TN, National Cotton Council of America, 1998;485.
28. Saha S., Raska D.A., Stelly D.M. Upland cotton (Gossypium hirsutum L.) × Hawaiian cotton (G. tomentosum Nutt. ex Seem.) F1 hybrid hypoaneuploid chromosome substitution series. J. Cotton Science. 2006;10:263-272.
29. Saha S., Raska D.A., Stelly D.M., Manchali S., Gutierrez O.A. Hypoaneuploid chromosome substitution F1 hybrids of Gossypium hirsutum L. × G. mustelinum Miers ex Watt. J. Cotton Science. 2013;17: 102-114.
30. Saha S., Stelly D.M. Chromosomal location of Phosphoglucomutase 7 locus in Gossypium hirsutum. J. Hered. 1994;85:35-39.
31. Saha S., Stelly D.M., Makamov A.K., Ayubov M.S., Raska D., Guitierrez O.A., Manchali S., Jenkins J.N., Deng D., Abdurakhmonov I.Y. Molecular confirmation of Gossypium hirsutum chromosome substitution lines. Euphytica. 2015;205:459-473. DOI 10.1007/s10681-015- 1407-2.
32. Saha S., Wu J., Jenkins J.N., McCarty J.C., Jr., Gutierrez O.A., Stelly D.M., Percy R.G., Raska D.A. Effect of Chromosome substitutions from Gossypium barbadense L. 3-79 into G. hirsutum L. TM-1 on agronomic and fiber traits. J. Cotton Science. 2004;8:162-169. DOI 10.1007/s00122-012-1965-9.
33. Samora P.J., Stelly D.M., Kohel R.J. Localization and mapping of the Le1 and Gl2 loci of cotton (Gossypium hirsutum L.). J. Hered. 1994; 85(2):152-157.
34. Sanamyan M.F. Cytogenetic effect of treatment of cotton seeds with thermal neutrons. Tsitologiya i genetika = Cytology and Genetics. 2003a;3:49-54. (in Russian)
35. Sanamyan M.F. Evaluation of the effect of pollen irradiation on karyotype variability in M1 cotton plants. Genetika = Genetics (Moscow). 2003b;39(7):947-955. (in Russian)
36. Sanamyan M.F. Evaluation of the effect of pollen irradiation on karyotype variability in M2 cotton plants. Genetika = Genetics (Moscow). 2003c;39(8):1081-1090. (in Russian)
37. Sanamyan M.F. Cytogenetic characterization of cotton haploid. Sbornik statey, posvyashchennyy 80-letiyu akademika A.A. Abdullaeva “Ғўzaning duneviy khilma-khilligi genofondi fundamental va amaliy tadkikotlar asosi” [Coll. The article is devoted to 80th anniversary of Acad. A.A. Abdullayev “Cotton in view of fundamental and applied studies of gene diversity in the world”]. Tashkent, 2010;121-124. (in Uzbek)
38. Sanamyan M.F., Musayev D.A. Detection and cytological study of aneuploid and euploid plants with chromosome translocations in cotton Gossypium hirsutum L. Genetika = Genetics (Moscow). 1990;26(3):506-515. (in Russian)
39. Sanamyan M.F., Petlyakova J.E., Musaev D.A. The development and a cytogenetic study of monosomics of Gossypium hirsutum L. Biologia Plantarum. 2000;43:193-197.
40. Sanamyan М.F., Petlyakova J., Rakhmatullina E.M., Sharipova E. Ch. 10. Cytogenetic collection of Uzbekistan. (Ed. I.Y. Abdurakhmonov) World Cotton Germplasm Resources. Croatia: InTech, 2014;247-287. DOI 10.5772/56978.
41. Sanamyan M.F., Petlyakova J.E., Sharipova E.A., Abdurakhmonov I.Y. Cytogenetic characteristics of new monosomic stocks of cotton (Gossypium hirsutum L.). Genetics Research International. 2011;12. http://dx.doi.org/10.4061/2011/273642.
42. Sears E.R. Misdivision of univalents in common wheat. Chromosoma. 1952;4:535-550.
43. Sears E.R. The aneuploids of common wheat. Research Bul. Univ. Missouri, College Agricul., Agricul. Exper. Station. 1954;(572): 1-58.
44. Stelly D.M. Localization of the Le2 locus of cotton (Gossypium hirsutum L.). J. Hered. 1990;81(3):193-197.
45. White T.G., Endrizzi J.R. Tests for the association of marker loci with chromosomes in Gossypium hirsutum L. by the use of aneuploids. Genetics. 1965;51:605-612.
46. Yu J.Z., Kohel R.J., Fang D.D., Cho J., Van Deynze A., Ulloa M., Hoffman S.M., Pepper A.E., Stelly D.M., Jenkins J.N., Saha S., Kumpatla S.P., Shah M.R., Hugie W.V., Percy R.G. A high- density simple sequence repeat and single nucleotide polymorphism genetic map of the tetraploid cotton genome. G3 (Bethesda). 2012;2:43-58. DOI 10.1534/g3.111.001552.
Review
Views:
694
Email this article 