Стрессовые системы Escherichia coli и их роль в реакциях на воздействие терагерцового излучения


https://doi.org/10.18699/VJ16.206

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Аннотация

Изложены результаты последних лет по изучению реакции стрессовых систем Escherichia coli в ответ на нетермическое воздействие терагерцового (ТГц) излучения. Наиболее простым и удобным объектом для изучения воздействия ТГц излучения на живые объекты является бактерия E. сoli. Это связано с ее высокой изученностью как на молекулярно-генетическом, так и на метаболическом уровне, а также c возможностью создания на основе промоторов ее стресс-активируемых генов и репортерного белка GFP геносенсорных конструкций. Введение этих конструкций в клетки E. coli позволяет исследовать реакцию конкретной стрессовой системы бактерии на ТГц излучение по интенсивности синтеза белка GFP, легко определяемого флуорометрически. В работе представлены данные литературных источников и собственные результаты по нетермическому воздействию ТГц излучения на конкретные стрессовые системы E. coli. Обсуждаются экспериментальные данные, полученные с использованием геносенсоров E. coli/pKatG-GFP, E. coli/pCopA-GFP и E. coli/pEmrR-GFP, которые являются маркерами генных сетей E. coli, активирующихся в условиях окислительного стресса, при нарушении гомеостаза ионов меди и в присутствии антисептиков соответственно. Обзор проведенных исследований показал, что нетермическое воздействие ТГц излучения индуцирует в клетках E. coli генные сети окислительного стресса и поддержания гомеостаза меди, но не влияет на активность стрессовых систем защиты от антибиотиков, протонофоров и супероксид-анионов. Наличие динамических особенностей в развитии стрессорного ответа у геносенсоров E. coli/ pKatG-GFP и E. coli/pCopA-GFP на ТГц излучение в сравнении с естественными индукторами позволяет предположить специфичность ответа систем окислительного стресса и поддержания гомеостаза меди в реакции адаптации клеток E. coli к ТГц излучению.


Об авторах

С. Е. Пельтек
Федеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук»
Россия
Новосибирск, Россия


Е. В. Демидова
Федеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук»
Россия
Новосибирск, Россия


В. М. Попик
Федеральное государственное бюджетное учреждение науки Институт ядерной физики им. Г.И. Будкера Сибирского отделения Российской академии наук
Россия
Новосибирск, Россия


Т. Н. Горячковская
Федеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук»
Россия
Новосибирск, Россия


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