Intraspecific variability of Puccinia triticina on hexaploid Triticum species and Aegilops trivialis
https://doi.org/10.18699/VJ17.28-o
Abstract
The analysis of pathogen intraspecific differentiation is a necessary stage for the development of a strategy of wheat leaf rust resistance breeding. To characterize the structure of the populations, virulence to Thatcher lines with leaf rust resistance genes (TcLr) and microsatellite markers may be used. The purpose of the present research is to study the intraspecific variability of the fungus Puccinia triticina Erikss. on Triticum L. hexaploid species and Aegilops trivialis (Zhuk.) Migusch. et Chak. using SSR-markers. Leaves of Triticum aestivum L., T. compactum Host., T. macha Dekapr. et Menabde, T. petropavlovskyi Udacz. et Migusch., T. spelta L., T. sphaerococcum Perc., T. vavilovii Jakubz. and Ae. trivialis with uredinia were collected on the experimental field of the Dagestan experiment station of VIR (Derbent) in 2014. For SSR-analysis 109, monopustule isolates previously characterized for virulence to 20 TcLr-lines were used. As a result of 18 microsatellite loci polymorphism analysis, 16 genotypes were identified. Genetic similarity between collections of isolates from the hexaploid types of wheat was significantly higher based on microsatellite markers than based on virulence. Microsatellite analysis confirmed a high similarity between Derbent isolates of P. triticina coming from the hexaploid Triticum species (including isolates from common wheat) and Aegilops trivialis.
About the Authors
E. I. Gultyaeva
All-Russian Institute of Plant Protection, St. Petersburg, Pushkin
Russian Federation
Russian Federation
E. L. Shaydayuk
All-Russian Institute of Plant Protection, St. Petersburg, Pushkin
Russian Federation
Russian Federation
I. A. Kazartsev
All-Russian Institute of Plant Protection, St. Petersburg, Pushkin
Russian Federation
Russian Federation
References
1. Balloux F., Lehmann L., de Meeus T. The population genetics of clonal and partially clonal diploids. Genetics. 2003;164:1635-1644.
2. Berlyand-Kozhevnikov V.M., Dmitriev A.P., Budashkina E.B., Shitova I.T., Reiter V.G. Ustoychivost pshenitsy k buroy rzhavchine (geneticheskoe raznoobrazie populyatsiy griba i rasteniya-khozyaina) [Wheat resistance to leaf rust: The genetic diversity in populations of the fungus and host plant]. Novosibirsk: Nauka Publ., 1978. (in Russian)
3. Boguslavskiy R.L., Golik O.V. Rod Aegilops L. kak geneticheskiy resurs selektsii [The genus Aegilops as a genetic resource for breeding]. Kharkov, 2004. (in Russian)
4. Bolton M.D., Kolmer J.A., Garvin D.F. Wheat leaf rust caused by Puccinia triticina. Mol. Рlant Рathol. 2008;9(5):563-575. DOI 10.1007/ s10681-011-0361-x.
5. Dmitriev A.P., Mikhailova L.A., Shelomova K.F., Derevyankin A.I. Investigation of the ranges of races and genotypes in the Derbent population of Puccinia recondita Rob. ex Desm. in 1972–1973. Miko logiya i fitopatologiya = Mycology and Phytopathology. 1976; 10(4):302-306. (in Russian)
6. Dorofeev V.F., Udachin R.A., Semenova L.V., Novikova M.V., Grad-chaninova O.D., Shitova I.P., Merezhko A.F., Filatenko A.A. Pshenitsy mira [Wheat of the world]. Leningrad: Kolos Publ., 1987. (in Russian)
7. Duan X., Enjalbert J., Vautrin D., Solignac C., Giraut T. Isolation of 12 microsatellite loci, using an enrichment protocol, in the phytopathogenic fungus Puccinia triticina. Mol. Ecol. Notes. 2003;3:65-67. DOI 10.1046/j.1471-8286.2003.00350.x.
8. Goncharov N.P. Sravnitelnaya genetika pshenits i ikh sorodichey [Comparative genetics of wheats and related species]. Novosibirsk: Geo Publ., 2012. (in Russian)
9. Gultyaeva E.I., Aristova M.K., Shaydayuk E.L., Mironenko N.V., Kazartsev I.A., Ahmetova A., Kosman E. Genetic differentiation of Рuc cinia triticina Еrikss. in Russia. Genetika = Genetics (Moscow). 2017а;53(9):1053-1060. DOI 10.7868S0016675817070037. (in Russian)
10. Gultyaeva E.I., Baranova O.A., Dmitriev A.P. Virulence and population structure of Puccinia triticina in the Russian Federation in 2007. Vestnik zashchity rasteniy = Plant Protection News. 2009;4:333-338. (in Russian)
11. Gultyaeva E.I., Shaydayuk E.L., Goncharov N.P., Аkhmetova A., Abdullaev K.M., Belousova M.H., Kosman E. Virulence of Puccinia triticina on Triticum and Aegilops species. Australas. Plant Рathol. 2016;45(2):155-163. DOI 10.1007/s13313-016-0395-6.
12. Gultyaeva E.I., Shaydayuk E.L., Kazartsev I.A. Population structure of Рuccinia triticina on tetraploid wheat species. Mikologiya i fitopatologiya = Mycology and Phytopathology. 2017б;51(5):299-304. (in Russian)
13. Gultyaeva E.I., Shaydayuk E.L., Kazartsev I.A., Aristova M.K. Structure of Russian populations of Puccinia triticina Eriks. Vestnik zashchity rasteniy = Plant Protection News. 2015;3(85):5-10. (in Russian)
14. Halkett F., Simon J.C., Balloux F. Tackling the population genetics of clonal and partially clonal organisms. Trends Ecol. Evol. 2005;20: 194-201. DOI 10.1016/j.tree.2005.01.001.
15. Justesen A.F., Ridout C.J., Hovmøller M.S. The recent history of Puccinia striiformis f. sp. tritici in Denmark as revealed by disease incidence and AFLP markers. Plant Pathol. 2002;51:13-23. DOI 10.1046/j.0032-0862.2001.00651.x.
16. Kolmer J.A., Jin Y., Long D.L. Wheat leaf and stem rust in the United States. Aust. J. Agric. Res. 2007;58:631-638.
17. Kolmer J.A., Liu J.Q. Virulence and molecular polymorphism in international collections of the wheat leaf rust fungus Puccinia triticina. Phytopathology. 2000;90(4):427-436. DOI 10.1094/PHYTO. 2000.90.4.427.
18. Kolmer J.A., Liu J.Q., Siem M. Virulence and molecular polymorphism in Puccinia recondita f. sp. tritici in Canada. Phytopathology. 1995; 85:276-285.
19. Kolmer J.A., Ordoñez M.E. Genetic differentiation of Puccinia triticina populations in Central Asia and the Caucasus. Phytopathology. 2007;97:1141-1149. DOI 10.1094/PHYTO-97-9-1141.
20. Liu M., Rodrigue N., Kolmer J. Population divergence in the wheat leaf rust fungus Puccinia triticina is correlated with wheat evolution. Heredity. 2014;112:443-453. DOI 10.1038/hdy.2013.123.
21. Long D.L., Kolmer J.A. A North American system of nomenclature for Puccinia recondita f. sp. tritici. Phytopathology. 1989;79:525-529. Mikhailova L.A. Genetika vzaimootnosheniy vozbuditelya buroy rzhav chiny i pshenitsy. Pod red. akad. RASKhN M.M. Levitina [M.M. Levitin (Ed.). The genetics of the relationships between the brown rust causative agent and wheat]. St.-Petersburg: VIZR Publ., 2006. (in Russian)
22. Mikhailova L.A. Puccinia recondita Rob. ex Desm. f. sp. tritici – Wheat leaf (brown) rust. A.N. Afonin, S.L. Greene, N.I. Dzyubenko, A.N. Frolov (Eds.). Interactive Agricultural Ecological Atlas of Russia and Neighboring Countries: Economic Plants and their Diseases, Pests and Weeds. 2008. Available at http://www.agroatlas.ru/en/ content/diseases/Tritici/index.html. (in Russian)
23. Mikhailova L.A., Gultyaeva E.I., Mironenko N.V. Methods of study of the population structure of the wheat leaf rust causative agent Puccinia recondita Rob. ex Desm. f. sp. tritici. Immuno-geneticheskie metody sozdaniya ustoychivykh k vrednym organizmam sortov [Immunogenetic methods of the development of pest-resistant varieties]. St.-Petersburg, 2000. (in Russian)
24. Mikhaylova L.A., Metreveli T.G. Population structure of Puccinia recondita Rob. ex Desm. f. sp. tritici on different wheat species. Mikologiya i fitopatologiya = Mycology and Phytopathology. 1986; 20:138-142. (in Russian)
25. Mitrofanova O.P., Strelchenko P.P., Konarev A.V., Balfourier F. Genetic differentiation of hexaploid wheat inferred from analysis of micro-satellite loci. Russian Journal of Genetics. 2009;45(11):1351-1559. DOI 10.1134/S102279540911009X. (in Russian)
26. Mikhaylova L.A., Vasil’ev S.V. Geographic ranges of populations of the wheat leaf rust agent. Mikologiya i fitopatologiya = Mycology and Phytopathology. 1985;19(2):158-163. (in Russian)
27. Ordoñez M.E., Kolmer J.A. Simple sequence repeat diversity of a worldwide collection of Puccinia triticina from durum wheat. Phytopathology. 2007;97:574-583. DOI 10.1094/PHYTO-97-5-0574.
28. Park R.F., Jahoor A., Felsenstein F.G. Population structure of Puccinia recondita in Western Europe during 1995 as assessed by variability in pathogenicity and molecular markers. Phytopathology. 2000;148: 169-179. DOI 10.1046/j.1439-0434.2000.00458.x.
29. Szabo L.S., Kolmer J.A. Development of simple sequence repeat markers for the plant pathogenic rust fungus Puccinia triticina. Mol. Ecol. 2007;7:708-710. DOI 10.1111/j.1471-8286.2007.01686.x.
30. Vavilov N.I. Nauchnye osnovy selektsii pshenitsy [Scientific bases of wheat breeding]. Moscow–Leningrad: Selkhozgiz Publ., 1935. (in Russian)
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