РЕДЕРИВАЦИЯ ПУТЕМ ТРАНСПЛАНТАЦИИ ЭМБРИОНОВ ЛИНИЙ ЛАБОРАТОРНЫХ МЫШЕЙ И КРЫС

Редеривация позволяет очистить колонии лабораторных мышей и крыс от специфических патогенов и перевести их в SPF (specified pathogen free) статус. В данной работе приведены результаты редеривации двух уникальных линий крыс, селекционированных в ИЦиГ СО РАН: ручных крыс-пасюков, крыс с наследственной индуцированной стрессом артериальной гипертензией (НИСАГ) и линии мышей ICR. SPF-статус редеривированных крыс был подтвержден при помощи животных-индикаторов, называемых также сентинелами (sentinel animals). В статье предложена оптимизированная модель редеривации лабораторных животных, которая включает в себя набор эмбриотехнологических методов, таких, как замораживание и криоконсервация эмбрионов, их очистка путем отмывки в стерильных средах, культивирование 48 ч и, наконец, трансплантация реципиентам (SPF-статуса). В результате применения этой модели по отношению к мышам линии ICR удалось получить 39 потомков, рожденных в условиях SPF-вивария. Следует отметить, что эффективность процедуры вполне соответствует международным стандартам, причем все три линии демонстрировали характерный фенотип после прохождения всех процедур редеривации.

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