При поддержке: This work was supported by the Russian Federation Ministry of Science and Higher Education, RF President’s grant MK-6575.2018.4. The development of the protocol for obtaining recombinant D3 domains was supported by the Russian Science Foundation, project 17-74-10146. The design of the recomibinant D3_Sof domain of the Sof’in-Ru TBEV strain was supported by the same project. The production and purification of chimeric ch14D5 antibody was supported by the Russian Science Foundation, project 16-14-00083. The production of viral RNA and cDNA was supported by the Russian Foundation for Basic Research, project 18-44-540021.