GENOME-WIDE SNP ALLELOTYPING OF HUMAN COHORTS BY POOLED DNA SAMPLES
Abstract
The research concerns the task of identification of contrasted single nucleotide polymorphisms (SNPs) obtained in genome-wide pooled allelotyping of 16 human cohorts, comprising healthy and ill persons, by the nested case–control approach. The genotyping platform was the Illumina Omni1S chip with 1.2 million markers. The mean pooled sample size was about 200 individuals. The candidate selection was based on statistical comparison of allele frequencies in a “case–control” study. Samples of ill patients show significant deviations from healthy persons in the numbers of significantly differing polymorphisms. The variance of allele frequencies among repeats in a single cohort was less than that in random choice of pairs from different cohorts.
About the Authors
V. N. Babenko
Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia
Novosibirsk National Research State University, Novosibirsk, Russia
Russian Federation
Russian Federation
V. N. Maximov
Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia
Institute of Therapy SB RAMS, Novosibirsk, Russia
Russian Federation
Russian Federation
E. V. Kulakova
Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia
Russian Federation
Russian Federation
E. S. Safronova
Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia
Novosibirsk National Research State University, Novosibirsk, Russia
Russian Federation
Russian Federation
M. I. Voevoda
Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia
Novosibirsk National Research State University, Novosibirsk, Russia
Institute of Therapy SB RAMS, Novosibirsk, Russia
Russian Federation
Russian Federation
E. I. Rogaev
Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia
Institute of Genetics RAS, Moscow, Russia
Russian Federation
Russian Federation
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