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Вавиловский журнал генетики и селекции

Расширенный поиск

Сомаклональная изменчивость у видов рода Saccharum: раскрытие ее потенциала в современных условиях

https://doi.org/10.18699/vjgb-26-42

Аннотация

Гибридизация различных местных сортов или диких видов сельскохозяйственных культур способствует генетической рекомбинации и приводит к созданию улучшенных сортов, в особенности у культур, размножающихся половым путем. В отличие от этого, у культур с бесполым размножением, таких как сахарный тростник (гибрид Saccharum spp.), генетическая рекомбинация посредством гибридизации затруднена из-за самоили перекрестной несовместимости (низкой фертильности). Такие сельскохозяйственные культуры могут быть улучшены за счет сомаклональной изменчивости с помощью методов культуры ткани. Сахарный тростник, являющийся одним из основных источников мирового производства сахара и биоэтанола, из-за недостаточной устойчивости к биотическим и абиотическим стрессовым факторам несет значительные потери урожая. Несмотря на эффективность методов культивирования in vitro, сомаклональная изменчивость остается недостаточно изученной в программах селекции сахарного тростника. Для решения проблем, появляющихся у сахарного тростника в условиях меняющейся динамики окружающей среды, в настоящем обзоре критически оценивается роль сомаклональной изменчивости в создании сортов сахарного тростника, рассмотрены ее основные механизмы, практическое применение и факторы, влияющие на ее возникновение. Кроме того, обсуждаются ограничения и проблемы практического применения этого метода при создании сортов, не позволяющие адекватно использовать его в современных селекционных работах. Использование потенциала сомаклональной изменчивости в сочетании с передовыми технологиями позволит преодолеть данные ограничения и удовлетворить растущие потребности в производстве сахара и биотоплива и в биоэнергетической промышленности.

Об авторах

С. Мунир
Ядерный институт сельского хозяйства (NIA); Ядерный институт сельского хозяйства и биологии
Пакистан

Группа биотехнологий сахарного тростника, Ядерный институт сельского хозяйства (NIA);

Отдел селекции и генетики растений, Ядерный институт сельского хозяйства и биологии

Тандо Джам, Фейсалабад



М. А.Б. Джаффар
Ядерный институт сельского хозяйства (NIA)
Пакистан

Группа биотехнологий сахарного тростника

Тандо Джам



Ш. Ясмин
Ядерный институт сельского хозяйства (NIA)
Пакистан

Группа биотехнологий сахарного тростника

Тандо Джам



М. Т. Хан
Национальный институт биотехнологии и генной инженерии
Пакистан

Отдел сельскохозяйственной биотехнологии

Фейсалабад



И. А. Хан
Ядерный институт сельского хозяйства (NIA)
Пакистан

Группа биотехнологий сахарного тростника

Тандо Джам



Список литературы

1. Abdullah, Smiullah, Khan F.A., Iftikhar R., Raza M.M., Aslam R., Hammad G., Ijaz A., Zafar W., Ijaz U. Detection of somaclonal variation in micropropagated plants of sugarcane and SCMV screening through ELISA. J Agr Sci. 2013;5:199-208. doi 10.5539/jas.v5n4p199

2. Abo-Elwafa A., Bakheit B.R., El-Taib A.B., Noby N.Y. Evaluation of some new somaclones of sugarcane for yield and quality. SVU-IJAS. 2021;3:129-139. doi 10.21608/svuijas.2021.59073.1073

3. Abreu I.S., Carvalho C.R., Clarindo W.R. Massal induction of Carica papaya L. ‘Golden’ somatic embryos and somaclone screening by flow cytometry and cytogenetic analysis. Cytologia. 2014;79:475- 484. doi 10.1508/cytologia.79.475

4. Ahmad K., Ming R. Harnessing genetic tools for sustainable bioenergy: a review of sugarcane biotechnology in biofuel production. Agriculture. 2024;14:1312. doi 10.3390/agriculture14081312

5. Ahuja M.R. Somaclonal genetics of forest trees. In: Jain S.M., Brar D.S., Ahloowalia B.S. (Eds) Somaclonal Variation and Induced Mutations in Crop Improvement. Dordrecht: Kluwer Academic, 1998; 105-121

6. Ali A., Naz S., Alam S., Iqbal J. In vitro induced mutation for screening of red rot (Colletotrichum falcatum) resistance in sugarcane (Saccharum officinarum). Pak J Bot. 2007;39(6):1979-1994

7. Almeida F.A., Santa-Catarina C., Silveira V. Somatic embryogenesis in sugarcane (Saccharum spp.). In: Ramírez-Mosqueda M.A. (Ed.) Somatic Embryogenesis. Methods in Molecular Biology. New York: Humana, 2022;83-95. doi 10.1007/978-1-0716-2485-2_7

8. Azizi P., Hanafi M.M., Sahebi M., Harikrishna J.A., Taheri S., Yassoralipour A., Nasehi A. Epigenetic changes and their relationship to somaclonal variation: a need to monitor the micropropagation of plantation crops. Funct Plant Biol. 2020;47(6):508-523. doi 10.1071/FP19077

9. Azu E., Elegba W., Asare A.T., Asare K., Akama C., Asare P., Annor C., Azure S., Danso K. Efficient callus-mediated system for commercial production of sugarcane (Saccharum spp.) planting material in Ghana. Afr J Biotechnol. 2022;21:208-217. doi 10.5897/AJB2021.17440

10. Bairu M.W., Fennell C.W., van Staden J. The effect of plant growth regulators on somaclonal variation in Cavendish banana (Musa AAA cv. ‘Zelig’). Sci Hortic. 2006;108:347-351. doi 10.1016/j.scienta.2006.01.039

11. Bairu M.W., Aremu A.O., Staden J.V. Somaclonal variation in plants: causes and detection methods. Plant Growth Regul. 2011;63:147173. doi 10.1007/s10725-010-9554-x

12. Barret P., Brinkman M., Beckert M. A sequence related to rice Pong transposable element displays transcriptional activation by in vitro culture and reveals somaclonal variations in maize. Genome. 2006; 49:1399-1407. doi 10.1139/g06-109

13. Bennetzen J.L., Ma J., Devos K.M. Mechanisms of recent genome size variation in flowering plants. Ann Bot. 2005;95(1):127-132. doi 10.1093/aob/mci008

14. Bidabadi S.S., Jain S.M. Cellular, molecular, and physiological aspects of in vitro plant regeneration. Plants. 2020;9(6):702. doi 10.3390/plants9060702

15. Bottcher A., Domingues-Junior A.P., Souza L.P., Tohge T., Araújo W.L., Fernie A.R., Mazzafera P. Sugarcane cell suspension reveals major metabolic changes under different nitrogen starvation regimes. Bragantia. 2021;80:e2921. doi 10.1590/1678-4499.2021-0009

16. Budeguer F., Enrique R., Perera M.F., Racedo J., Castagnaro A.P., Noguera A.S., Welin B. Genetic transformation of sugarcane, current status and future prospects. Front Plant Sci. 2021;12:768609. doi 10.3389/fpls.2021.768609

17. Dalvi S.G., Vasekar V.C., Yadav A., Tawar P.N., Dixit G.B., Prasad D.T., Deshmukh R.B. Screening of promising sugarcane somaclones for agronomic traits, and smut resistance using PCR amplification of inter transcribed region (ITS) of Sporisorium scitamineum. Sugar Tech. 2012;14:68-75. doi 10.1007/s12355-011-0132-y

18. D’Amato F., Buiatti M., Raghavan V., Johri B.M., Bhojwani S.S., Rangaswamy N.S., de Nettancourt D., Devreux M. Cytology, cytogenetics and plant breeding. In: Reinert J., Bajaj Y.P.S. (Eds) Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture. Springer, 1977;343-464. doi 10.1007/978-3-662-02279-5_3

19. Daub M.E. Tissue culture and the selection of resistance to pathogens. Annu Rev Phytopathol. 1986;24:159-186. doi 10.1146/annurev.py.24.090186.001111

20. De Klerk G.J., Arnholdt-Schmitt B., Lieberei R., Neumann K.H. Regeneration of roots, shoots and embryos: physiological, biochemical and molecular aspects. Biol Plant. 1997;39:53-66. doi 10.1023/A:1000304922507

21. Di Pauli V., Fontana P.D., Lewi D.M., Felipe A., Erazzú L.E. Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars. J Genet Eng Biotechnol. 2021;19(1):171. doi 10.1186/s43141-021-00270-8

22. Doležel J., Valárik M., Vrána J., Lysák M.A., Hřibová E., Bartoš J., Gasmanová N., Doleželová M., Šafář J., Šimková H. Molecular cytogenetics and cytometry of bananas (Musa spp). In: Jain S.M., Swennen R. (Eds) Banana Improvement: Cellular, Molecular Biology, and Induced Mutations. Enfield: Science Publishers Inc, 2004;229-244

23. Doule R.B. Cane yield and quality characters of some promising somaclonal variants of sugarcane. Sugar Tech. 2006;8:191-193. doi 10.1007/BF02943660

24. Duncan R.R. Tissue culture-induced variation and crop improvement. Adv Agron. 1997;58:201-240. doi 10.1016/S0065-2113(08)60256-4

25. Duta-Cornescu G., Constantin N., Pojoga D.M., Nicuta D., SimonGruita A. Somaclonal in micropropagation of variation-advantage or disadvantage the medicinal plants. Int J Mol Sci. 2023;24(1):838. doi 10.3390/ijms24010838

26. Etienne H., Bertrand B. Somaclonal variation in Coffea arabica: effects of genotype and embryogenic cell suspension age on frequency and phenotype of variants. Tree Physiol. 2003;23:419-426. doi 10.1093/treephys/23.6.419

27. Farahani F., Yari R., Masoud S. Somaclonal variation in Dezful cultivar of olive (Olea europaea subsp. europaea). Gene Conserve. 2011; 10:216-221

28. Food and Agriculture Organization. Agricultural production statistics 2010–2023. FAOSTAT database. 2024. https://www.fao.org/statistics/highlights-archive/highlights-detail/agricultural-production-statistics2010-2023/en

29. Gandonou C.B., Errabii T., Abrini J., Idaomar M., Senhaji N.S. Selection of callus cultures of sugarcane (Saccharum sp.) tolerant to NaCl and their response to salt stress. Plant Cell Tissue Organ Cult. 2006;87:9-16. doi 10.1007/s11240-006-9113-3

30. Goldner W., Thom M., Maretzki A. Sucrose metabolism in sugarcane cell suspension cultures. Plant Sci. 1991;73(2):143-147. doi 10.1016/ 0168-9452(91)90021-Y

31. Heinz D.J. Isozyme prints for variety identification. ISSCT Breeders’ Newsletter. 1969;24:8

32. Heinz D.J., Mee G.W.P. Morphologic, cytogenetic, and enzymatic variation in Saccharum species hybrid clones derived from callus tissue. Am J Bot. 1971;58:257-262. doi 10.1002/j.1537-2197.1971.tb09971.x

33. Hoy J.W., Bischoff K.P., Milligan S.B., Gravois K.A. Effect of tissue culture explant source on sugarcane yield components. Euphytica. 2003;129:237-240. doi 10.1023/A:1021928823445

34. Hung Y.-H., Liu F., Zhang X.-Q., Xiao W., Hsieh T.-F. Sexual and nonsexual reproduction: inheritance and stability of epigenetic variations and consequences for breeding application. In: Gallusci P., Bucher E., Mirouze M. (Eds) Plant Epigenetics Coming of Age for Breeding Applications. Advances in Botanical Research. Academic Press, 2018;88:117-163. doi 10.1016/bs.abr.2018.09.002

35. Israeli Y., Reuveni O., Lahav E. Qualitative aspects of somaclonal variations in banana propagated by in vitro techniques. Sci Hort. 1991;48(1-2):71-88. doi 10.1016/0304-4238(91)90154-Q

36. Israeli Y., Lahav E., Reuveni O. In vitro culture of bananas. In: Gowen S. (Ed.) Bananas and Plantains. World Crop Series. Springer, 1995;147-178. doi 10.1007/978-94-011-0737-2_6

37. Jackson J.A., Lyndon R.F. Habituation: cultural curiosity or developmental determinant? Physiol Planta. 1990;79:579-583. doi 10.1111/j.1399-3054.1990.tb02120.x

38. Jalaja N.C., Sreenivasan T.V., Pawar S.M., Bhoi P.G., Garker R.M. Co 94012 – a new sugarcane variety through somaclonal variation. Sugar Tech. 2006;8:132-136. doi 10.1007/BF02943647

39. Karp A. The role of growth regulators in somaclonal variation. Br Soc Plant Growth Regul Ann Bull. 1992;2:1-9

40. Karp A. Origins, causes and uses of variation in plant tissue cultures. In: Vasil I.K., Thorpe T.A. (Eds) Plant Cell and Tissue Culture. Springer, 1994;139-151. doi 10.1007/978-94-017-2681-8_6

41. Khan I.A., Khatri A. Plant regeneration via organogenesis or somatic embryogenesis in sugarcane: histological studies. Pak J Bot. 2006; 38(3):631-636

42. Khan I.A., Dahot M.U., Seema N., Bibi S., Khatri A. Genetic variability in plantlets derived from callus culture in sugarcane. Pak J Bot. 2008;40(2):547-564

43. Khan I.A., Dahot M.U., Seema N., Yasmeen S., Bibi S., Raza G., Khatri A., Naqvi M.H. Direct regeneration of sugarcane plantlets: a tool to unravel genetic heterogeneity. Pak J Bot. 2009;41(2): 797-814

44. Khan I.A., Seema N., Raza S., Yasmeen S. Comparative performance of sugarcane somaclones and exotic germplasm under agro-climatic conditions of Tando Jam. Pak J Bot. 2015;47(3):1161-1166

45. Khan M.T., Khan I.A., Yasmeen S., Seema N., Nizamani G.S. Field evaluation of diverse sugarcane germplasm in agroclimatic conditions of Tandojam, Sindh. Pak J Bot. 2018;50:1441-1450

46. Khan S.J., Khan M.A., Ahmed H.K., Khan R.D., Zafar Y. Somaclonal variation in sugarcane through tissue culture and subsequent screening for salt tolerance. Asian J Plant Sci. 2004;3:330-334. doi 10.3923/ajps.2004.330.334

47. Khan S., Saeed B., Kauser N. Establishment of genetic fidelity of in ¬vitro raised banana plantlets. Pak J Bot. 2011;43:233-242

48. Krikorian A.D., Irizarry H., Cronauer-Mitra S.S., Rivera E. Clonal fidelity and variation in plantain (Musa AAB) regenerated from vegetative stem and floral axis tips in vitro. Ann Bot. 1993;71:519-535. doi 10.1006/anbo.1993.1068

49. Krishna H., Alizadeh M., Singh D., Singh U., Chauhan N., Eftekhari M., Sadh R.K. Somaclonal variations and their applications in horticul tural crops improvement. 3 Biotech. 2016;6:54. doi 10.1007/s13205-016-0389-7

50. Krishnamurthi M., Tlaskal J. Fiji disease resistant Saccharum officinarum var. Pindar sub-clones from tissue cultures. In: Proceedings XV Congress, International Society of Sugar Cane Technologists. 1974;15(1):130-137

51. Kumar P., Agarwal A., Tiwari A., Lal M., Jabri M. Possibilities of development of red rot resistance in sugarcane through somaclonal variation. Sugar Tech. 2012;14:192-194. doi 10.1007/s12355-012-0138-0

52. Kumari K., Lal M., Saxena S. Enhanced micropropagation and tiller formation in sugarcane through pretreatment of explants with thidiazuron (TDZ). 3 Biotech. 2017;7:282. doi 10.1007/s13205-017-0910-7

53. Larkin P.J., Scowcroft W.R. Somaclonal variation – a novel source of variability from cell culture for plant improvement. Theor Appl Genet. 1981;60:197-214. doi 10.1007/BF02342540

54. Letham D., Gollnow B. Regulators of cell division in plant tissues. XXX. Cytokinin metabolism in relation to radish cotyledon expansion and senescence. J Plant Growth Regul. 1985;4:129-145. doi 10.1007/BF02266951

55. Leva A., Rinald L.M.R. Somaclonal variation. In: Thomas B., Murray B.G., Murphy D.J. (Eds) Encyclopedia of Applied Plant Sciences. Cambridge: Academic Press, 2017;468-473. doi 10.1016/B978-0-12-394807-6.00150-7

56. Leva A., Petruccelli R., Rinaldi L. Somaclonal variation in tissue culture: a case study with olive. In: Leva A., Rinaldi L.M.R. (Eds) Recent Advances in Plant in vitro Culture. London: Intech Open, 2012. doi 10.5772/50367

57. LoSchiavo F., Pitto L., Giuliano G., Torti G., Nuti-Ronchi V., Marazziti D., Vergara R., Orselli S., Terzi M. DNA methylation of embryogenic carrot cell cultures and its variations as caused by mutation, differentiation, hormones and hypomethylating drugs. Theor Appl Genet. 1989;77:325-331. doi 10.1007/BF00305823

58. Mahmud K., Nasiruddin K.M., Hossain M.A., Hassan L. Screening sugarcane somaclones and their parent varieties against red rot (Colletotrichum falcatum) and assessment of variability by RAPD and SSR markers. SAARC J Agric. 2015;13(2):173-182. doi 10.3329/sja.v13i2.26578

59. Manchanda P., Kaur A., Gosal S.S. Somaclonal variation for sugarcane improvement. In: Gosal S.S., Wani S.H. (Eds) Biotechnologies of Crop Improvement. Vol. 1. Springer, 2018;299-326. doi 10.1007/978-3-319-78283-6_9

60. Marques J.C., Gasi F., Lourenço S.R. Biofuel in the automotive sector: viability of sugarcane ethanol. Sustainability. 2024;16:2674. doi 10.3390/su16072674

61. Mathur S. Conservation of biodiversity through tissue culture. Res Rev J Microbiol Biotechnol. 2013;2(3):1-6

62. Metcalfe C.J., Li J., Giorgi D., Piperidis N., Aitken K.S. Flow cytometric characterisation of the complex polyploid genome of Saccharum officinarum and modern sugarcane cultivars. Sci Rep. 2019;9: 19362. doi 10.1038/s41598-019-55652-3

63. Mujib A., Banerjee S., Dev Ghosh P. Callus induction, somatic embryogenesis and chromosomal instability in tissue culture raised hippeastrum (Hippeastrum hybridum cv. United Nations). Propag Ornam Plants. 2007;7(4):169-174

64. Naheed R., Arfan M., Farhat F., Ijaz S., Khalid H. Acclimatization of drought tolerance with somaclonal variants of sugarcane (Saccharum officinarum L.). Adv Life Sci. 2020;8(1):57-62

65. Nair N.V., Selvi A., Sreenivasan T.V., Pushpalatha K.N. Molecular diversity in Indian sugarcane cultivars as revealed by randomly amplified DNA polymorphisms. Euphytica. 2002;127:219-225. doi 10.1023/A:1020234428681

66. Naz S., Ali A., Siddique A. Somatic embryogenesis and plantlet formation in different varieties of sugarcane (Sacchrum officinarum L.) HSF-243 and HSF-245. Sarhad J Agric. 2008;24(4):593-598

67. Nickell L.G., Maretzki A. Growth of suspension cultures of sugarcane cells in chemically defined media. Physiol Plant. 1969;22:117-125. doi 10.1111/j.1399-3054.1969.tb07847.x

68. Nikam A.A., Devarumath R.M., Ahuja A., Babu H., Shitole M.G., Suprasanna P. Radiation-induced in vitro mutagenesis system for salt tolerance and other agronomic characters in sugarcane (Saccharum officinarum L.). Crop J. 2015;3:46-56. doi 10.1016/j.cj.2014.09.002

69. Nogueira G.F., Luis Z.G., Salles L.A., Pasqual M., ScherwinskiPereira J.E. High-efficiency organogenesis and evaluation of the regenerated plants by flow cytometry of a broad range of Saccharum spp. hybrids. Biologia. 2022;77:3265-3278. doi 10.1007/s11756-022-01176-7

70. Nwauzoma A.B., Jaja E.T. A review of somaclonal variation in plantain (Musa spp): mechanisms and applications. J Appl Biosci. 2013;67: 5252-5260. doi 10.4314/jab.v67i0.95046

71. Oliveira A.C., Pasqual M., Bruzi A.T., Pio L.A., Mendonça P.M., Soares J.D. Flow cytometry reliability analysis and variations in sugarcane DNA content. Genet Mol Res. 2015;14(2):7172-7183. doi 10.4238/2015.June.29.11

72. Oloriz M.I., Gil V., Rojas L., Veitia N., Hofte M., Jimenez E. Selection and characterisation of sugarcane mutants with improved resistance to brown rust obtained by induced mutation. Crop Pasture Sci. 2011;62(12):1037-1044

73. Patade V.Y., Suprasanna P., Bapat V.A. Gamma irradiation of embryogenic callus cultures and in vitro selection for salt tolerance in sugarcane (Saccharum officinarum L.). Agric Sci China. 2008;7(9):1147- 1152. doi 10.1016/S1671-2927(08)60158-3

74. Petolino J.F., Roberts J.L., Jayakumar P. Plant cell culture: a critical tool for agricultural biotechnology. In: Vinci V.A., Parekh S.R. (Eds) Handbook of Industrial Cell Culture. Humana Press, 2003;243-258. doi 10.1007/978-1-59259-346-0_10

75. Petrov D.A. Evolution of genome size: new approaches to an old problem. Trends Genet. 2001;17:23-28. doi 10.1016/s0168-9525(00)02157-0

76. Phillips R.L., Kappler S.M., Olhof R. Genetic instability of plant tissue cultures: breakdown of normal controls. Proc Natl Acad Sci USA. 1994;91(12):5222-5226. doi 10.1073/pnas.91.12.5222

77. Rajeswari S., Thirugnanakumar S., Anandan A., Krishnamurthi M. Somaclonal variation in sugarcane through tissue culture and evaluation for quantitative and quality traits. Euphytica. 2009;168: 71-80. doi 10.1007/s10681-009-9889-4

78. Seema N., Khan I.A., Raza S., Yasmeen S., Bibi S., Nizamani G.S. Assessment of genetic variability in somaclonal variation in sugarcane. Pak J Bot. 2014;46:2107-2111

79. Sengar A., Thind K., Kumar B., Pallavi M., Gosal S.S. In vitro selection at cellular level for red rot resistance in sugarcane (Saccharum sp.). Plant Growth Regul. 2009;58:201-209. doi 10.1007/s10725-009-9368-x

80. Shahid M.T., Khan F.A., Saeed A., Fareed I. Variability of red rot-resistant somaclones of sugarcane genotype S97US297 assessed by RAPD and SSR. Genet Mol Res. 2011;10:1831-1849. doi 10.4238/vol10-3gmr1122

81. Shahid M.T.H., Khan F.A., Saeed A. Development of somaclones in sugarcane genotype BF-162 and assessment of variability by random amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) markers in selected red rot resistant somaclones. Afr J Biotechnol. 2012;11(15):3502-3513. doi 10.5897/AJB11.2729

82. Sharma S., Bryan G., Winfield M., Millam S. Stability of potato (Solanum tuberosum L.) plants regenerated via somatic embryos, axillary bud proliferated shoots, microtubers and true potato seeds: a comparative phenotypic, cytogenetic and molecular assessment. Planta. 2007;226:1449-1458. doi 10.1007/s00425-007-0583-2

83. Shen X., Chen J., Kane M., Henny R. Assessment of somaclonal variation in Dieffenbachia plants regenerated through indirect shoot organogenesis. Plant Cell Tissue Organ Cult. 2007;91:21-27. doi 10.1007/s11240-007-9273-9

84. Shomeili M., Nabipour M., Meskarbashee M., Memari H.R. Evaluation of sugarcane (Saccharum officinarum L.) somaclonals tolerance to salinity via in vitro and in vivo. HAYATI J Biosci. 2011;18(2):91-96. doi 10.4308/hjb.18.2.91

85. Singh G., Sandhu S., Meeta M., Singh K., Gill R., Gosal S.S. In vitro induction and characterization of somaclonal variation for red rot and other agronomic traits in sugarcane. Euphytica. 2008;160:35-47. doi 10.1007/s10681-007-9531-2

86. Smiullah, Khan F.A., Abdullah, Afzal A., Javed M.A., Iqbal Z., Iftikhar R., Wattoo J.I. In vitro regeneration, detection of somaclonal variation and screening for mosaic virus in sugarcane (Saccharum spp.) somaclones. Afr J Biotechnol. 2012;11:10841-10850. doi 10.5897/AJB11.4073

87. Sobhakumari V.P. Assessment of somaclonal variation in sugarcane. Afr J Biotechnol. 2012;11:15303-15309. doi 10.5897/AJB12.1627

88. Sun S., Zhong J., Li S., Wang X. Tissue culture-induced somaclonal variation of decreased pollen viability in torenia (Torenia fournieri Lind.). Bot Stud. 2013;54:36. doi 10.1186/1999-3110-54-36

89. Tawar P.N., Sushir K.V., Meti N.T. Somaclonal variation an aid for sugarcane improvement. Int J Curr Res Biosci Plant Biol. 2016;3: 47-55.

90. Thorat A.S., Sonone N.A., Choudhari V.V., Devarumath R.M., Babu K.H. Plant regeneration from cell suspension culture in Saccharum officinarum L. and ascertaining of genetic fidelity through RAPD and ISSR markers. 3 Biotech. 2017;7:16. doi 10.1007/s13205-016-0579-3

91. Thorat A.S., Sonone N.A., Choudhari V.V., Devarumath R.M., Babu K.H. Plant regeneration from direct and indirect organogenesis and assessment of genetic fidelity in Saccharum officinarum using DNA-based markers. Biosci Biotechnol Res Commun. 2018;11: 60-69. doi 10.21786/bbrc/11.1/9

92. Thumjamras S., Iamtham S., Lersrutaiyotin R., Prammanee S. Identification of sugarcane somaclones derived from callus culture by SSR and RAPD marker analysis. Thai J Agric Sci. 2011;44(5): 71-76

93. Tican A., Câmpeanu G., Chiru N., Ivanovici D. Using of unconventional methods for obtaining somaclonal variations, having as goal making of new potato varieties with resistance at diseases and pests. Rom Biotechnol Lett. 2008;13(4):3791-3798

94. Wagih M.E., Ala A., Musa Y. Regeneration and evaluation of sugarcane somaclonal variants for drought tolerance. Sugar Tech. 2004;6: 35-40. doi 10.1007/BF02942615

95. Warren G. The regeneration of plants from cultured cells and tissues. In: Stafford A., Warren G. (Eds) Plant Cell and Tissue Culture. Milton Keynes: Open University Press, 1991;82-100

96. Weising K., Nybom H., Wolff K., Kahl G. DNA Fingerprinting in Plants: Principles, Methods, and Applications. New York: CRC Press, 2005

97. Yadav P.V., Suprasanna P., Gopalrao K.U., Anant B.V. Molecular profiling using RAPD technique of salt and drought tolerant regenerants of sugarcane. Sugar Tech. 2006;8:63-68. doi 10.1007/BF02943744

98. Yasmeen S., Rajput M.A., Khan I.A., Hasseny S.S. Induced mutations and somaclonal variations in three sugarcane (Saccharum officinarum L.) varieties. Pak J Bot. 2017;49:955-964

99. Zayova E., Vassilevska I.R., Kraptchev B., Stoeva D. Somaclonal variations through indirect organogenesis in eggplant (Solanum melongena L.). Biol Divers Conserv. 2010;3:1-5


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